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EC number: 215-410-7 | CAS number: 1325-87-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- The range-finding study was conducted between 2 March 1999 and 6 March 1999 and the definitive study between 10 May 1999 and 14 May 1999
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: LF 961482
- Expiration date of the lot/batch: Not stated
- Purity test date: 05 February 1999
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: Room temperature in the dark
- Stability under test conditions: stable
- Solubility and stability of the test substance in the solvent/vehicle: Poorly soluble so WAFs (water accommodated fractions) prepared
- Reactivity of the test substance with the solvent/vehicle of the cell culture medium: not stated
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: None
Prior to the addition of the test material the dechlorinated tap water was stirred by magnetic stirrer using a stirring rate such that a vortex depth of approximately 25% of the overall height of the water column was achieved. An amount of test material (2.0 g) was added to the vortex of 20 litres of dechlorinated tap water to give the 100 mg/L loading rate. This loading rate was stirred for 23 hours.
The stirring was stopped after 23 hours and the mixture allowed to stand for 1 hour. The WSF was then removed by filtration through 0.2 µm cartridge filters.
The concentration and stability of the test material in the test solutions were verified by chemical analysis at 0, 24 and 96 hours - Analytical monitoring:
- yes
- Details on sampling:
- Water samples were taken from the control and each replicate test vessel at 0, 24 and 96 hours for quantitative analysis. Duplicate samples were taken and stored frozen (approximately -20· C) for further analysis , if necessary. The method of analysis, stability, recovery and test solution analyses are described in the report
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
-Prior to the addition of the test material the dechlorinated tap water was stirred by magnetic stirrer using a stirring rate such that a vortex depth of approximately 25% of the overall height of the water column was achieved. An amount of test material (2.0 g) was added to the vortex of 20 litres of dechlorinated tap water to give the 100 mg/L loading rate. This loading rate was stirred for 23 hours.
The stirring was stopped after 23 hours and the mixture allowed to stand for 1 hour. The WSF was then removed by filtration through 0.2 µm cartridge filters. - Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Strain: Oncorhynchus mykiss
- Source: Reputable commercial supplier
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): 4.5 cm (sd = 0.2)
- Weight at study initiation (mean and range, SD): 1.14 g (sd = 0.12
- Method of breeding: N/a
- Maintenance of the brood fish: N/a
ACCLIMATION
- Acclimation period: 30 days
- Acclimation conditions (same as test or not): yes
- Type and amount of food during acclimation: commercial trout pellets discontinued 24h prior to start of definitive study
- Feeding frequency during acclimation: daily
- Health during acclimation (any mortality observed): Health good, ca. 3% mortality prior to start of study
QUARANTINE (wild caught)
- Duration:
- Health/mortality:
FEEDING DURING TEST
- Food type:
- Amount:
- Frequency: - Test type:
- semi-static
- Water media type:
- other: dechlorinated tapwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 207 mg/L total hardness
- Test temperature:
- 14 ± 0.5· C throughout the study
- pH:
- 7.7 - 7.9
- Dissolved oxygen:
- 9.3 - 9.8 mg/L
- Nominal and measured concentrations:
- WAF's prepared at a loading rate of 100 mg/L.
- Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- NOELR
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Validity criteria fulfilled:
- yes
- Conclusions:
- The acute toxicity of the test material to the freshwater fish rainbow trout (Oncorhynchus mykiss) has been investigated and gave a 96-Hour LLR50* of greater than 100 mg/L loading rate WSF. Correspondingly the No Observed Effect Loading Rate was 100 mg/L loading rate WSF.
Reference
Description of key information
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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