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Toxicity to microorganisms

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Description of key information

The 3-h EC50 value of AC-6000 in domestic activated sludge is >100 mg/L; this value will be used in the assessment.

Key value for chemical safety assessment

Additional information

A GLP-compliant OECD 209 guideline study with predominantly domestic sludge is available for AC-6000 (NOTOX B.V., 2007). Activated sludge (0.7 g/L suspended solids, 2 replicates) was exposed to a nominal concentration of 100 mg/L for 3 hours.

The test concentration was prepared by stirring the test solution with a target dose level of 200 mg/L for 24 hours before exposure. The growth inhibition rate of the test substance was 9%. The 3-h EC50 value is >100 mg/L, this effect concentration is larger than the water solubility.