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Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

AC-6000 is characterised as “not readily biodegradable”.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The biodegradation of AC-6000 by microorganisms was studied in accordance with OECD guideline 301C (Modified MITI test (I)) (Kurume Laboratory, 2006a).

In this GLP-compliant study 30 mg/L activated sludge (prepared from samples taken from industrial and city sewage treatment plants, surface water and surface soil) was exposed to 100 mg/L AC-6000 for 28 days in triplicate. The controls included an inoculum blank (3 replicates), an abiotic sterile control (1 replicate) and a positive control (aniline, 3 replicates). After 7, 14, 21 and 28 days the removal of oxygen expressed as biochemical oxygen demand (BOD) were calculated. In addition, the concentrations of test substance were analysed with high performance liquid chromatography (HPLC).

After 28 days the BOD of AC-6000 ranged from 10.4 to 12.2 mg, the degradation ranged from -12 to -5%, the average degradation was -9% (measured as O2-consumption), therefore the degradation was assumed to be 0%.

The degradation of the reference substance was 69% (measured as O2-consumption) after 28 days.

AC-6000 was not biodegraded by microorganisms under the test conditions of this study.