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EC number: 252-744-2
CAS number: 35836-73-8
a reverse gene mutation assay in bacteria, performed according to
Guideline OECD 471 and in compliance with GLP, strains of Salmonella
typhimurium (TA1535, TA1537, TA98, TA100 and TA102) were exposed to the
test item, Nopol, at the concentrations below.
1 (plate-incorporation method):
TA1535, TA1537, TA98, TA100 and TA102: 5, 16, 50, 160, 500, 1600 and
5000 μg/plate, with and without S9-mix
2 (plate-incorporation method without S9 mix; preincubation method with
TA1535, TA1537, TA98, TA100 and TA102: 25, 50, 100, 200, 400, 800 and
1600 μg/plate, with and without S9-mix
3 (preincubation method):
TA1535, TA1537, TA98, TA100 and TA102: 6.25, 12.5, 25, 50, 100, 200 and
400 μg/plate, with S9-mix
activation system was S9 fraction prepared from liver homogenates of
male Sprague Dawley rats induced with Aroclor 1254. Vehicle and positive
controls were also included.
Experiment 1, evidence
of toxicity was observed at 160 and/or 500 μg/plate
and above in all strains in the absence and presence of S-9. In
Experiment 2, evidence
of toxicity was observed at 800 μg/plate
and above in all strains in the absence of S-9 and at 400 μg/plate
and above in the presence of S-9 in all strains and in strain TA1537 in
the absence of S-9 only. Since mutation data were only available for
four concentrations in the presence of S-9 for all tester strains due to
toxicity, a further Experiment (Experiment 3) was performed. In
Experiment 3, evidence of toxicity was
observed at 200 μg/plate
and above in all strains.
mean numbers of revertant colonies fell within acceptable ranges for
vehicle control treatments, and were elevated by positive control
significant increases in the frequency of revertant colonies were
recorded for any of the bacterial strains, at any dose level either with
or without metabolic activation.
the test item is not considered as mutagenic in this bacterial system.
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