Registration Dossier

Ecotoxicological information

Toxicity to terrestrial arthropods

Currently viewing:

Administrative data

Endpoint:
toxicity to terrestrial arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
29 Jun - 19 Oct 1998
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1998
Report Date:
1998

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: EPPO Guideline No. 170 (1992)
GLP compliance:
yes
Application method:
contact

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): AEF122006 00 1C990006

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Method of test material application: single topical dose
- Body part: ventral thorax
- Volume of test solution applied: 1.0 μL
- Controls: 1.0 μL acetone
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): acetone

Test organisms

Test organisms (species):
Apis mellifera
Animal group:
Hymenoptera (honeybees)
Details on test organisms:
TEST ORGANISM
- Common name: Honey bee
- Source: The bees used for testing were taken from colonies on the land of Hoechst Schering AgrEvo GmbH, Frankfurt am Main.
- Disease free: yes

Study design

Study type:
laboratory study
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
25 - 26 °C
Humidity:
68- 80 %
Photoperiod and lighting:
constant darkness during the test period (Observations were made under light.)
Details on test conditions:
TEST SYSTEM
- Test container / cage (material, size): wire mesh cages, which were closed at both ends with a cork
Number of test series: 3 ( Control, test - and reference substance)
Number of test concentrations: Test compound: 2; Reference substance: 5
Number of replicates: 5
Number of bees per replicate: 10

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Dead bees were recorded 24, 48 and 72 hours after application of the test or reference substance.

Nominal and measured concentrations:
50.4 and 100.7 μg technical substance/bee
Reference substance (positive control):
yes
Remarks:
Triazophos: 40.9 %w/w

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
LD50
Effect conc.:
> 100.7 other: μg technical substance per bee
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: highest test concentration
Details on results:
- No mortalities were observed during the test
- No unforeseen circumstances were observed, which may have affected the quality or integrity of this study.
- The study was performed as a limit test, but two concentrations were tested
Results with reference substance (positive control):
- Results: See "Any other information on results incl. tables", LD50 not reported
- Although the values for the reference substance were slightly above normal they remain within the range of biological variation

Any other information on results incl. tables

Number of dead bees for reference substance

μg product / bee

Total number of dead bees after:

 

24 h

48 h

72 h

0.1

0

0

0

0.2

2

5

5

0.3

39

39

39

0.4

48

48

48

0.5

47

47

47

Applicant's summary and conclusion