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Diss Factsheets
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EC number: 678-448-7 | CAS number: 3084-53-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Eye irritation
Administrative data
- Endpoint:
- eye irritation: in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 021
- Report date:
- 2021
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 437 (Bovine Corneal Opacity and Permeability Test Method for Identifying i) Chemicals Inducing Serious Eye Damage and ii) Chemicals Not Requiring Classification for Eye Irritation or Serious Eye Damage)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- trimethylsulfanium bromide
- EC Number:
- 678-448-7
- Cas Number:
- 3084-53-5
- Molecular formula:
- C3H9BrS
- IUPAC Name:
- trimethylsulfanium bromide
Constituent 1
- Specific details on test material used for the study:
- Lot No.: 21MR1-2
Purity: 99%
Test animals / tissue source
- Species:
- other: Bovine cornea
- Details on test animals or tissues and environmental conditions:
- SOURCE OF COLLECTED EYES
- Source: Spear Products Inc., 520 Springfield street, coopersburg, PA 18036.
- Number of animals: 9 corneas
- Selection and preparation of corneas: Eyes were carefully examined for defects (opacity, scratches, pigmentation, etc) shortly after arrival to the facility. Any eyes with defects were omitted from the study and discarded. The tissue surrounding the eye ball was carefully pulled away, and the cornea was excised, leaving a rim of sclera approximately 2 to 3 mm wide. The isolated corneas were maintained in HBSS until all were dissected.
Test system
- Vehicle:
- water
- Controls:
- yes, concurrent positive control
- yes, concurrent negative control
- Amount / concentration applied:
- TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 750 uL
- Concentration (if solution): 20% w/v - Duration of treatment / exposure:
- 4 hours
- Duration of post- treatment incubation (in vitro):
- 90 mins
- Number of animals or in vitro replicates:
- 3
- Details on study design:
- REMOVAL OF TEST SUBSTANCE
- Number of washing steps after exposure period: washed at least 3 times with MEM containing phenol red until no color change occurred.
METHODS FOR MEASURED ENDPOINTS:
- Corneal opacity:
- Corneal permeability: passage of sodium fluorescein dye measured with the aid of [UV/VIS spectrophotometry / microtiter plate reader] (OD490)
SCORING SYSTEM: In Vitro Irritancy Score (IVIS)
DECISION CRITERIA: the decision criteria as indicated in the TG was used.
Results and discussion
In vitro
Results
- Irritation parameter:
- in vitro irritation score
- Value:
- -1
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Other effects / acceptance of results:
- TMSBr meets the requirements of EPA category III and UN GHS no category for the BCOP test.
Proper conduct of the BCOP was confirmed via a positive response with imidazole and a negative response with 0.9% sterile NaCl.
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- TMSBr is not irritating to eyes in the BCOP test.
- Executive summary:
The Bovine corneal opacity & permeability assay was performed using isolated bovine cornea, according to OECD Guideline 437 under GLP.
TMSBr is not irritating to eyes in the BCOP test.
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