1,1,2,3,3-pentafluoro-3-(heptafluoropropoxy)prop-1-ene

Skin sensitisation

Administrative data

Endpoint:

skin sensitisation: in chemico

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 442C (In Chemico Skin Sensitisation: Direct Peptide Reactivity Assay (DPRA))

Deviations:

no

Remarks:

No deviations occurred that impacted the integrity of the study.

GLP compliance:

yes

Type of study:

direct peptide reactivity assay (DPRA)

Test material

Test material information

Constituent 1

Reference substance name:

1,1,2,3,3-pentafluoro-3-(heptafluoropropoxy)prop-1-ene

EC Number:

817-766-4

Cas Number:

91891-42-8

Molecular formula:

C6F12O

IUPAC Name:

1,1,2,3,3-pentafluoro-3-(heptafluoropropoxy)prop-1-ene

Test material form:

liquid

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 3M Company, Lot MA-3-HO-ANL1600356
- Expiration date of the lot/batch: No data
- Purity test date: No data

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: At room temperature, protected from light.
- Stability under test conditions: Stable
- Solubility and stability of the test substance in the solvent/vehicle: Soluble in vehicle (acetonitrile)

TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing: The test article was vortexed in acetonitrole for approximately 1 minute.

In chemico test system

Details on the study design:

Skin sensitisation (In chemico test system) - Details on study design:
The test article was diluted in acetonitrile to yield a 100 mM solution. The cysteine peptide was prepared by weighing an appropriate amount of the peptide to achieve a 0.667 mM concentration. The lysine peptide was prepared by weighing an appropriate amount of the peptide to achieve a 0.667 mM concentration. The reaction mix for cysteine peptide had a 1:10 test peptide to reference article ratio (0.5 mM cysteine to 5 mM reference article). The reaction mix for lysine peptide had a 1:50 peptide to reference article ratio (0.5 mM lysine to 25 mM reference article). Reactions for test and reference articles were run in triplicate. The positive control used in this assay was cinnamic aldehyde prepared at a concentration of 100 mM. The positive control was reacted with the peptides in the same fashion as the test article. There were three sets of reference controls of acetonitrile run at different points throughout the assay. The test artice and controls were incubated with each peptide solution (cysteine or lysine) for 24 hours. Following incubation, the extent of peptide depletion was analyzed using High Performance Liquid Chromatography (HPLC) coupled with ultra-violet (UV) spectrometric detection. Test article peptide depletion was compared with solvent controls.

Results and discussion

Positive control results:

71.56% Cysteine depletion, 58.36% Lysine depletion.

In vitro / in chemico

Results

Results 1

Key result

Run / experiment:

mean

Parameter:

other: % Cysteine Depletion

Remarks:

Compared to solvent controls.

Value:

94.21

Vehicle controls validity:

valid

Negative controls validity:

not examined

Positive controls validity:

valid

Results 2

Key result

Run / experiment:

mean

Parameter:

other: % Lysine Depletion

Remarks:

Compared to solvent controls

Value:

0.86

Vehicle controls validity:

valid

Negative controls validity:

not examined

Positive controls validity:

valid

Other effects / acceptance of results:

- Acceptance criteria met for solvent control: Yes
- Acceptance criteria met for positive control: Yes

Applicant's summary and conclusion

Interpretation of results:

Category 1B (indication of skin sensitising potential) based on GHS criteria

Conclusions:

Based on the results of the study, the test article is predicted to have skin sensitization potential within this stage of the skin sensitization adverse outcome pathway (AOP).

Executive summary:

The sensitization potential of the test article was examined using the Direct Peptide Reactivity Assay (DPRA). The test was performed under GLP conditions and followed the guidance in OECD 442C (2015). The test article was solubilized in acetonitrile to yield a final concentration of 100 mM. The cysteine peptide was prepared at 0.667 mM in cysteine Reaction buffer and the lysine peptide was prepared at 0.667 mM in Lysine Reaction buffer as outlined in OECD 442C. The reaction mix for cysteine peptide had a 1:10 test peptide to reference article ratio (0.5 mM cysteine to 5 mM reference article). The reaction mix for lysine peptide had a 1:50 peptide to reference article ratio (0.5 mM lysine to 25 mM reference article). Reactions for test and reference articles were run in triplicate. Vehicle control reactions were also made with acetonitrile containing no reference or test articles. After 24 ± 2 hours incubation DPRA samples were assayed for peptide depletion

via HPLC/UV. The separations module used in this assay was a Waters 2695 HPLC system. This system consisted of a solvent management system for the mobile phases and a sample management system for the test article and controls. The HPLC system was coupled to a photodiode array detector set at 220 nm. The dimensions of the column used were 2.1 mm x 100 mm x 3.5 micron. A percent depletion DPRA of 47.54% (94.21% Cysteine, 0.86% Lysine) was observed for the test article. Based on the results of the study, the test article is predicted to have skin sensitization potential within this stage of the skin sensitization adverse outcome pathway (AOP).