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EC number: 253-668-2
CAS number: 37788-55-9
In the blank control, one of the Daphnia was immobilised (see
Nominal Concentration in mg/L
Immobility 24 hours
Immobility 48 hours
In the blank control, none of the Daphnia was immobilised (see
* The two lowest concentrations (4.6 mg/L and 10 mg/L) were
excluded from the evaluation of the biological results, because they
showed no significant toxicity compared to the control.
Two valid experiments were performed.
The 1stexperiment was performed using one concentration
of 100 mg/L (limit test). For the test concentration and the blank
control, 20 Daphnia were exposed to the test item for 48 hours in a
static test system. After 24 and 48 hours, the immobilised Daphnia were
In this experiment more than 10 % of the Daphnia in the test
concentration were immobilised and therefore a 2ndexperiment
was performed using 5 concentrations ranging from 4.6 to 100 mg/L (full
test). The test was carried out under the same conditions like the 1stexperiment.
The biological results base on the results of the second
The two lowest concentrations (4.6 mg/L and 10 mg/L) were excluded
from the evaluation of the biological results, because they showed no
significant toxicity compared to the control.
The two highest concentrations (46 mg/L and 100 mg/L) showed
toxicity between 5 and 15 % immobilisation. None of the animals was
immobilised in the blank control.
At the beginning and at the end of the test, the content of the
test item in the test solutions was determined
usingHPLC-UV-determination. The measured concentrations in the 3 highest
concentrations were between 98 % and 104 % of the nominal concentration
at the beginning and between 108 % and 115 % of the nominal
concentrations at the end of the test. Therefore, the determination of
the biological results was based on the nominal concentrations of the
Potassium dichromate K2Cr2O7(CAS
No. 7778-50-9) was used as positive controlin a current reference study
to assure that the test conditions are reliable.
The following results were determined for the test
itemN-(2-hydroxypropyl)imidazole, purified by distillation(species:Daphnia
48h-EC50> 100 mg/L
The immobilisation of 15 % at the concentration of 100 mg/L lay in
the same range as in the limit test (20 %), but the evaluation program
does not recognize this as a significant difference in comparison to the
control. Therefore, the NOEC was determined as≥the highest tested
A study according to OECD 202 was conducted.
Based on the test results the EC50, 48h for Daphnie magna can be stated
to be > 100 mg/L.
Thus the test material is not toxic towards aquatic invertebrates
according to the criteria of CPL.
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