1,1'-(benzene-1,3-diyldimethanediyl)bis(1H-pyrrole-2,5-dione)

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2018-01-29 to 2018-04-18

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Version / remarks:

2008

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Key result

Water solubility:

37.6 mg/L

Conc. based on:

test mat.

Incubation duration:

>= 48 - <= 72 h

Temp.:

20 °C

pH:

5.3

Table 2: Results of the preliminary test

phase separation procedure		Meass. Conc. [mg/L]
		Replicate 1	Replicate 2
centrifugation	10000 x g for 10 min	72.2	76.9
	20000 x g for 20 min	69.3	74.0
filtration	0.2 µm pore-size	69.1	73.5

Table 3: Procedural Control for the Filtration Procedure (preliminary test)

Procedural control	Meas. Conc. [mg/L]	Mean	RR [%]
from calibration	10.24872	10.22	-
	10.19639
filtrated	10.1841	-	99.6

Table 4: Recovery rates of the fortified samples (HPLC analysis):

Replicate No	1 x LOQ		10 x LOQ
	Calc. conc. [mg/L]	RR [%]	Calc. conc. [mg/L]	RR [%]
1	5.07	101	49.3	99
2	5.07	101	49.7	99
3	5.08	102	50.2	100
4	5.11	102	50.3	101
5	5.09	102	49.9	100
Mean	5.08	102	49.9	100
SD	0.02		0.4
CV [%]	0.37		0.85

Calc. conc. = Calculated concentration, exact weight and dilution factor of 10 taken into account

RR = Recovery rate, related to the fortified concentration

SD = Standard deviation

CV = Coefficient of variation

Conclusions:

In the present study conducted according to OECD guideline 105 (1995) the water solubility of m-Xylylenebismaleimide was determined by the flask method. The water solubility of the test item is 37.6 mg/L at 20°C and a pH of 5.3.

Description of key information

-study according to OECD guideline 105, GLP, water solubility of m-Xylylenebismaleimide is 37.6 mg/L at 20°C and a pH of 5.3.

Key value for chemical safety assessment

Water solubility:

37.6 mg/L

at the temperature of:

20 °C

Additional information

In the present study conducted according to OECD guideline 105 (1995) the water solubility of m-Xylylenebismaleimide was determined by the flask method. A preliminary test was performed in order to verify the planned study design. For this purpose 50 mg of the test item were mixed with 50 mL double distilled water in duplicate and stirred for 2 weeks. after this incubation aliquots of each mixture were centrifuged at 20 °C and 10000 x g for 10 min as well as at 20000 x g for 20 min and filtrated via syringe filters with a pore-size of 0.2 um, respectively. For each sample, 0.5 mL of the aqueous phase was transferred into a clear glass vial and checked for Tyndall-effects. For each sample, and thus for each used phase separation procedure, a Tyndall effect was observed. The least visible Tyndall effect was observed for the filtrated samples. The solubility was determined to be in the range of 70 mg/L for the filtrated samples, no significant loss of the test item was observed for the procedural control. For the definitive testing, filtration was used for the phase separation and slight Tyndall effects are deemed to be acceptable.

In the definitive test HPLC analysis after a preincubation time of 48 and 72 h revealed a water solubility of 37.6 mg/L at 20°C and a pH of 5.3.