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Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Endpoint:
bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2009
Report date:
2009

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 305 (Bioaccumulation in Fish: Aqueous and Dietary Exposure) -II: Minimised Aqueous Exposure Fish Test
Deviations:
no
Qualifier:
according to guideline
Guideline:
EPA OPPTS 850.1730 (Fish Bioconcentration Test)
Deviations:
no
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
Potassium 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctanesulphonate
EC Number:
261-818-3
EC Name:
Potassium 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctanesulphonate
Cas Number:
59587-38-1
Molecular formula:
C8H5F13O3S.K
IUPAC Name:
potassium 3,3,4,4,5,5,6,6,7,7,8,8,8-tridecafluorooctane-1-sulfonate
Test material form:
solid: particulate/powder
Details on test material:
- Purity: 97.9%

Test solutions

Vehicle:
yes
Remarks:
Dilution water

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)

Study design

Route of exposure:
other: Aqueous and feed
Test type:
flow-through
Water / sediment media type:
natural water
Total exposure / uptake duration:
56 d
Total depuration duration:
28 d

Test conditions

Hardness:
124 to 134 mg/L as CaCO3
Test temperature:
11.9 to 12.1°C
pH:
7.0 to 7.5
Dissolved oxygen:
7.8 to 10.3 mg/L (air saturation at 12°C = 8.9 mg/L)
Conductivity:
228 to 280 mhos/cm
Nominal and measured concentrations:
Aqueous
Nominal: 1 and 10 μg/L
Measured: 1.08 and 9.32 μg/L

Dietary
Nominal: 10 μg/kg
Measured: 13.1 μg/kg
Reference substance (positive control):
not specified

Results and discussion

Lipid content
Remarks on result:
other: The lipid content of test fish was not measured because lipids are not a relevant partitioning phase for the class of chemistry represented by the test substance.
Bioaccumulation factoropen allclose all
Key result
Conc. / dose:
1 µg/L
Type:
BCF
Value:
22 dimensionless
Basis:
other: whole fish
Calculation basis:
steady state
Remarks on result:
other: aqueous test
Key result
Conc. / dose:
10 µg/L
Type:
BCF
Value:
3 dimensionless
Basis:
other: Whole fish
Calculation basis:
steady state
Remarks on result:
other: aqueous test
Key result
Conc. / dose:
1 µg/L
Type:
BCF
Value:
36 dimensionless
Basis:
other: whole fish
Calculation basis:
kinetic
Remarks on result:
other: aqueous test
Key result
Conc. / dose:
10 µg/L
Type:
BCF
Value:
3 dimensionless
Basis:
other: Whole fish
Calculation basis:
kinetic
Remarks on result:
other: aqueous test
Key result
Conc. / dose:
10 other: μg/kg
Type:
BMF
Value:
0.295 dimensionless
Basis:
other: Whole fish
Calculation basis:
steady state
Remarks on result:
other: dietary test
Depurationopen allclose all
Key result
Parameter:
other: Time to 95% Depuration
Depuration time (DT):
23.5 d
Remarks on result:
other: Aqueous (1 μg/L)
Key result
Parameter:
other: Time to 95% Depuration
Depuration time (DT):
4.1 d
Remarks on result:
other: Aqueous (10 μg/L)
Key result
Parameter:
other: Time to 95% Depuration
Depuration time (DT):
17 d
Remarks on result:
other: Aqueous (10 μg/L)
Remarks:
Excluding day 63 depuration phase data
Rate constantsopen allclose all
Key result
Rate constant:
growth rate constant (d-1)
Remarks:
Aqueous (1 μg/L)
Value:
4.462
Key result
Rate constant:
growth rate constant (d-1)
Remarks:
Aqueous (10 μg/L)
Value:
0.452
Key result
Rate constant:
overall depuration rate constant (d-1)
Remarks:
Aqueous (1 μg/L)
Value:
0.125
Key result
Rate constant:
overall depuration rate constant (d-1)
Remarks:
Aqueous (10 μg/L)
Value:
0.727
Key result
Rate constant:
growth-corrected depuration rate constant (d-1)
Remarks:
Dietary (10 μg/kg)
Value:
0.029
Key result
Rate constant:
growth-corrected half-life (d)
Remarks:
Dietary (10 µg/kg)
Value:
23.5
Key result
Rate constant:
growth rate constant (d-1)
Remarks:
Dietary (10 µg)
Value:
0.057
Key result
Rate constant:
other:
Remarks:
Overall elimination rate (d-1)
Value:
0.057
Reported statistics:
Time to equilibrium was determined by ANOVA for each aqueous test substance concentration and the dietary test substance concentration

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
Bioconcentration and dietary biomagnification potential are low for test substance. Hence, it would not be considered a bioaccumulative compound.
Executive summary:

The study was conducted to evaluate bioconcentration and bioaccumulation of test substance in Rainbow Trout, Oncorhynchus mykiss according to guidelines, OECD 305 and US EPA.OPPTS 850.1730. Rainbow trout, Oncorhynchus mykiss, were exposed to test substance under flow-through conditions for 56 days during the uptake (exposure) phase of the study followed by a 28-day depuration phase in clean dilution water. Two nominal aqueous test substance concentrations (1 and 10 µg/L), a single nominal spiked diet concentration (10 µg/kg), and a dilution water control were used for testing with 60 fish per exposure treatment and control. An error in preparation of the aqueous test concentrations and delay in analytical confirmation of the test substance concentrations resulted in the day 1-13 uptake phase aqueous test substance concentrations being below the analytical method limit of detection of 0.06 µg/L. This error was corrected upon discovery as reflected in the aqueous test substance concentrations for study days 14 through 56. The mean, measured concentrations of test substance in the aqueous test solutions from days 14-56 were 1.08 µg/L and 9.32 µg/L and the measured test substance concentration in the spiked trout chow diet used for the duration of the dietary uptake phase of the study was 13.1 µg/Kg. One-day after initiation of the depuration phase there were no detectable levels of test substance present in the aqueous test solution test chambers. The dilution water control and the control trout chow diet contained no detectable concentrations of test substance during the study.

Fish tissue residue concentrations of test substance reached steady-state equilibrium within 42 days or earlier in both aqueous and dietary exposures. The k1 values (uptake rate constant) for days 14-56 of the aqueous exposures in whole fish based on measured tissue residues were 4.462 and 0.452 day-1 for the nominal 1 and 10 µg/L nominal aqueous test concentrations while the day 14-56 depuration rate constants, k2, in whole fish based on measured tissue residues were 0.125 and 0.177 day-1, respectively. After a maximum of 24 days of depuration, the measured concentrations in the whole fish were approximately 95% or less of the whole fish tissue concentrations at the end of the uptake phase. However, measured concentrations in whole fish at the end of the uptake phase on day 56 were already less than 50% of the maximum uptake phase concentration in whole fish for both aqueous test concentrations and the dietary exposure concentration of test substance. The test substance steady-state bioconcentration factors (BCF) for the nominal 1 and 10 µg/L aqueous test substance concentrations were 22 and 3, respectively. The respective test substance kinetic bioconcentration factors were 36 and 3, respectively, for the nominal 1 and 10 µg/L aqueous test substance concentrations. The dietary assimilation efficiency for test substance, the growth corrected half-life, and the dietary biomagnification factor (BMF) for the dietary exposure were 0.435, 23.5 days, and 0.295, respectively.

Based on existing regulatory schemes in the United States and Europe, these results demonstrate that the bioconcentration and dietary biomagnification potential are low and test substance would not be considered a bioaccumulative compound