N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

9 September 2004

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide

EC Number:

200-644-4

EC Name:

N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide

Cas Number:

67-16-3

Molecular formula:

C24H34N8O4S2

IUPAC Name:

N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

Not specified

Test solutions

Vehicle:

no

Details on test solutions:

To prepare the stock solution, the test item was dissolved or dispersed directly in M4 reconstituted water. The pH of this solution (M4) is 8.0 ± 0.5 and the sum of Ca and Mg ions in the solution is 2.5 mmol/L with a molar ratio of Ca to Mg ions of approximately 4:1, and of Na to K of 10:1. The obtained preparation was agitated mechanically for up to 24 hours in an attempt to completely dissolve or disperse the test item.

Test solutions was prepared by further dilution of the stock solution with M4 or directly from the test item. A solution containing a maximum of 100 mg/L of the test item plus two other range-finding concentrations was prepared. Test vessels was filled directly from the test solution containers immediately after preparation. The pH range considered as appropriate for a normal life of test organisms is circa 6 to 9.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

Daphnia magna STRAUS, clone 5.
Reason for this choice: most sensitive clone of the species recommended in OECD and EEC guidelines.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Post exposure observation period:

Animals were inspected for immobilization at TO, T24 and T48 hours.

Test conditions

Hardness:

272 mg/L at T0 and 289 mg/L at T48 on the control flask

Test temperature:

19,7°C mini
20.2°C maxi

pH:

pH = between 8.13 & 8.20 at T0
ph = between 8.35 & 8.37 at T48

Dissolved oxygen:

between 8.6 & 8.7 mg/L at T0
between 8.7 & 8.8 mg/L at T48

Salinity:

Not specified

Conductivity:

Not specified

Nominal and measured concentrations:

Nominal concentration of 1, 10 and 100 mg/L

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

No immobilisation was observed at tested concentrations.

Any other information on results incl. tables

Table I :physicochemical parameters

Control
Time	Hardness (mg/L)	Oxygen levels %	Oxygen levels (mg/l)          pH
T 0 hours T 48 hours	272 289	98 98	8,7 8,8	8,20 8,37
Highest tested concentration
Time                   Oxygen level %		Oxygen level (mg/L)	pH	Nominal charge rate (mg (L)
T 0 hours T 48 hours                   97		8,6 8,7	8,13 8,35	100 100

Temperature measured in water between T2 hours and TO time: 19.8°C.

Temperature recorded in a beaker of water dilution placed in the conditions of the study: minimum 19.7°C / maximum 20.2°C.

Table 2:Summary table of mortality

Immobilization (n on 5 daphnia) at each nominal rate (mg/L)

 

	0	1	10	100
	Replicat 1 2 3 4	Replicat 1 2 3 4	Replicat 1 2 3 4	Replicat 1 2 3 4
T 0 hour	0 0 0 0	0 0 0 0	0 0 0 0	0 0 0 0
T 24 hours	0 0 0 0	0 0 0 0	0 0 0 0	0 0 0 0
T 48 hours	0 0 0 0	0 0 0 0	0 0 0 0	0 0 0 0

 

Applicant's summary and conclusion

Conclusions:

The highest rate of charge without immobilisation at T48h was > 100 mg/L.
The lowest charge rate with 100% of immobilisation at T48h was > 100 mg / L.

Executive summary:

The highest rate of charge without immobilisation at T48h was > 100 mg/L.

The lowest charge rate with 100% of immobilisation at T48h was > 100 mg / L.

EC50s at 24 h and 48 h were > 100 mg/L (charge rate).