N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study without detailed documentation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2004

Report date:

2004

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide

EC Number:

200-644-4

EC Name:

N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide

Cas Number:

67-16-3

Molecular formula:

C24H34N8O4S2

IUPAC Name:

N,N'-[dithiobis[2-(2-hydroxyethyl)-1-methylvinylene]]bis[N-[(4-amino-2-methyl-5-pyrimidinyl)methyl]formamide

Test material form:

solid: particulate/powder

Sampling and analysis

Analytical monitoring:

not specified

Details on sampling:

Not specified

Test solutions

Vehicle:

not specified

Details on test solutions:

A solution containing a maximum of 100 mg/L of the test item plus two other range-finding concentrations was prepared. Test vessels (aquariums) was filled directly from the test solution containers immediately after preparation. The same vessels was used throughout the test. The pH range considered as appropriate for a normal life of test organisms is circa 6 to 9. Consequently, the tesst solution « with a pH < 5 R or > 0.2 was tested with pH adjustment.

Test organisms

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

Justification of this choice: the rainbow trout is a fish species generally accepted by regulatory authorities for this type of study and is recommended in OECD and EEC guidelines.
Origin: CIT, BP 563, 27005 Evreux, France; the eggs are obtained from Viviers de France, Pisciculture des Fontaines, 64490 Sarrance, France.
Allocation to study: animals was selected using a hand-net, without preference, and randomly assigned to test vessels.
Number of animals: four groups of seven fish.

Study design

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Test conditions

Hardness:

151 to 157 mg/L of CaCO3 in the control

Test temperature:

14.1 to 14.6°C

pH:

7.51 (mini) to 8.04 (maxi)

Dissolved oxygen:

6.9 mg/L (min) to 8.5 (max)

Salinity:

Not specified

Conductivity:

Not specified

Nominal and measured concentrations:

Nominal concentrations : 1, 10 & 100 mg/L

Details on test conditions:

Test solutions was not changed over the test period.
Light/dark cycle: 16/8 hours.
Duration of test: 96 hours.
Loading: up to 1g of fish per liter of test solution. The animals were between 4 and 6 cm in length.
Oxygen concentration: all attempts was made to maintain dissolved oxygen at or above 60% of the air saturation value at that temperature.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

behaviour

Effect concentrations 2

Key result

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 3

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 4

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Effect concentrations 5

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Results with reference substance (positive control):

Not specified

Any other information on results incl. tables

Sublethal observations / clinical signs:

 Table 1: Physicochemical paramters

Time (hours)		Temperature (°C)	pH	Oxygen content (mg/L)	Water hardness (mg/L of CaCO3)
Control
0		14.2	7.51	8.5	151
24		14.3	7.71	8.4
48		14.2	7.62	7.4
72		14.4	8.03	7.8
96		14.6	7.88	6.9	157
Highest nominal rate charge : 100mg/L
0		15.6	8.01	8.3
24		14.1	7.98	8.4
48		14.4	8.04	7.7
72		14.4	8.04	7.7
96		14.5	7.91	7.2

Table 2: Mortality summary

Observation time (hours)	Mortality at each nominal concentrations (mg/L) n fishes on 7 fishes
	control	1	10	100
0	0	0	0	0
24	0	0	0	0
48	0	0	0	0
72	0	0	0	0
96	0	0	0	0

Applicant's summary and conclusion

Conclusions:

The highest loading rate without clinical effect and mortality at T96h was 100 mg/L. The lowest loading rate with 100% mortality at T96h was> 100 mg / L.
LC50s at 24h, 48h, 72h and 96h were> 100 mg / L (loading rate).

Executive summary:

Acute short-term toxicity test on fish (rainbow trout) under static conditions was conducted accorting to guidline OCDE.

The highest loading rate without clinical effect and mortality at T96h was 100 mg/L. The lowest loading rate with 100% mortality at T96h was> 100 mg / L.

LC50s at 24h, 48h, 72h and 96h were> 100 mg / L (loading rate).