Sulfuric acid, mono-C16-20-alkyl esters, sodium salts

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11-13 Feb 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Remarks:

10/2013/DPL, Inspector for Chemical Substances, Lodz, Poland, 30.07.2013

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all test concentrations at exposure initiation, at renewal, and at exposure termination

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION:
Appropriate amounts of the test item were weighed in glass beakers, the content of each beaker was transferred into glass flask by multiple
washing with Elendt M7 medium. Each flask was filled up into total volume with Elendt M7 medium, and was sonicated for 30 minutes. All test item mixtures were nontransparent with particles on the surface. The content of flasks was left for 30 minutes in order to obtain the visually homogeneous fraction. This fraction was then collected using a glass tube from the middle part of each flask and split up into test vessels and for chemical analyses. The collected fractions were the Water Accommodated Fraction (WAF) and were used for exposure. After 24 h of exposure the test item concentrations were renewed to freshly prepared.

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: water flee
- Strain: Daphnia magna Straus
- Source: laboratory culture at the Institute of Industrial Organic Chemistry, Branch Pszczyna, Department of Ecotoxicology, Laboratory of Aquatic Toxicology
- Age at study initiation: < 24h, neonates
- Method of breeding: cultured in glass beakers with a capacity of 150 mL (one parent per vessel) containing 100 mL of Elend M7 medium, at room temperature. Daily cycle 16 h light : 8 h dark. The daphnids were fed with a suspension of algae; mixture of two species Pseudokirchneriella subcapitata and Desmodesmus subspicatus (in 2:1 ratio). Group B vitamins and micronutrients necessary for proper growth were supplied with the lyophilized suspension of Spirulina sp.
- Feeding during test: none

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

none

Test temperature:

18.4 - 19.7 ºC

pH:

7.41 – 7.59

Dissolved oxygen:

8.6 – 9.7 mg/L

Nominal and measured concentrations:

WAF of a loading of 0.0, 2.0, 4.3, 9.4, 20.7, 45.5, 100 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers of 150 mL
- Type (delete if not applicable): closed
- Aeration: no
- Renewal rate of test solution (frequency/flow rate): the test item concentrations were renewed to freshly prepared after 24 exposure
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
Elendt M7 medium

OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: daily cycle 16 h light : 8 h dark
- Light intensity: fluorescent light source

EFFECT PARAMETERS MEASURED: mobility after 24 and 48 hours

TEST CONCENTRATIONS
- Range finding study
- Test concentrations:
First preliminary test: 100, 10, 1.0, 0.1, 0 mg/L (filtrate of a loading concentrations)
Second preliminary test: 10, 50, 10 5, 0 mg/L (nominal, WAF)
Third preliminart test: 10, 5, 2.5, 1.0, 0 mg/L (nomina, WAF)

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

37.91 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF

Basis for effect:

mobility

Remarks on result:

other: 12.75 - 25.72 mg/L

Duration:

48 h

Dose descriptor:

other: EC20

Effect conc.:

18.58 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF

Basis for effect:

mobility

Remarks on result:

other: 0.25 - 38.16 mg/L

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

45.5 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

20.7 mg/L

Nominal / measured:

nominal

Conc. based on:

other: WAF

Basis for effect:

mobility

Results with reference substance (positive control):

EC50 (48 h) = 0.49 mg/L

Reported statistics and error estimates:

Probit method calculations and analysis by Fisher’s Exact Binominal Test with Bonferroni Correction.

Description of key information

48h EC50 (Daphnia magna, mobility) = 37.91 mg/L (nominal concentration, WAF)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

37.91 mg/L

Additional information

One short-term toxicity study with aquatic invertebrates is available for C16-20AS Na (CAS 91648-55-4). The semi-static test was performed with Daphnia magna according to OECD 202 (BASF, 2015). Daphnids were exposed to six test concentrations and control treatment prepared as WAF (nominal concentrations). Total organic carbon content was measured.

The number of immobile organisms was evaluated after 24 and 48 hours. The 48-hour EC50 value was 37.91 mg/L (nominal concentration, WAF).