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EC number: 234-165-7 | CAS number: 10576-12-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- data from handbook or collection of data
Data source
Referenceopen allclose all
- Reference Type:
- review article or handbook
- Title:
- determination of Skin sensitization for CAS no: 3710-84-7
- Author:
- HSDB
- Year:
- 2 017
- Bibliographic source:
- U.S. National Library of Medicine National Institutes of Health, Health & Human Services; 2017.
- Reference Type:
- secondary source
- Title:
- Robust Summary & Test Plans: Diethylhydroxylamine: Robust Summary
- Author:
- IUCLID Dataset-OECD SIDS
- Year:
- 2 006
- Bibliographic source:
- Robust Summary & Test Plans: Diethylhydroxylamine: Robust Summary; IUCLID Dataset-OECD SIDS; 2006 JANURY 13.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as mentioned below
- Principles of method if other than guideline:
- A Buehler test was conducted on male guinea pigs to assess the skin sensitization potential of chemical N,N-Diethylhydroxylamine (CAS no: 3710-84-7) .
- GLP compliance:
- not specified
- Type of study:
- Buehler test
- Justification for non-LLNA method:
- not specified
Test material
- Reference substance name:
- N,N-Diethylhydroxylamine
- Cas Number:
- 3710-84-7
- Molecular formula:
- C4H11NO
- IUPAC Name:
- N,N-Diethylhydroxylamine
- Test material form:
- liquid
- Details on test material:
- - Name of test material (as cited in study report): - N,N-Diethylhydroxylamine
Molecular formula: C4H11NO
- Molecular: 89.1369 g/mol
- Substance type: Organic
- Physical state: Liquid
- InChI: 1S/C4H11NO/c1-3-5(6)4-2/h6H,3-4H2,1-2H3
- Smiles: N(CC)(CC)O
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): - N,N-Diethylhydroxylamine
Molecular formula: C4H11NO
- Molecular: 89.1369 g/mol
- Substance type: Organic
- Physical state: Liquid
- InChI: 1S/C4H11NO/c1-3-5(6)4-2/h6H,3-4H2,1-2H3
- Smiles: N(CC)(CC)O
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Sex:
- male
- Details on test animals and environmental conditions:
- - Age at study initiation: young adult
Study design: in vivo (non-LLNA)
Induction
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: deionized water
- Concentration / amount:
- 30%
- Day(s)/duration:
- 3 weeks
- Adequacy of induction:
- other: the highest non-irritating dose concentration
Challenge
- No.:
- #1
- Route:
- epicutaneous, occlusive
- Vehicle:
- other: deionized water
- Concentration / amount:
- 30%
- Day(s)/duration:
- 48 hours
- Adequacy of challenge:
- highest non-irritant concentration
- No. of animals per dose:
- 15 guinea pigs
- Details on study design:
- MAIN STUDY
A. INDUCTION EXPOSURE
- No. of exposures: 9
- Exposure period: 48 hours
- Test groups: 10
- Control group: no data available
- Site: no data
- Frequency of applications: on alternate days until a total of nine dose applications was achieved
- Duration: 3 weeks
- Concentrations: 30%
B. CHALLENGE EXPOSURE
- No. of exposures: 1
- Day(s) of challenge: after 17 days
- Exposure period: 48 hours
- Test groups: 10
- Control group: 5
- Site: naive site
- Concentrations: 30%
- Evaluation (hr after challenge): 24 and 48 hours - Challenge controls:
- A naive control group of five animals was maintained under the same environmental conditions and was treated with the test material at challenge only.
- Positive control substance(s):
- yes
- Remarks:
- 0.08% Dinitrochlorobenzene (DNCB) in 95% ethyl alcohol
Results and discussion
In vivo (non-LLNA)
Results
- Reading:
- 1st reading
- Hours after challenge:
- 48
- Group:
- test chemical
- Dose level:
- 30%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Clinical observations:
- All guinea pigs appeared active and healthy throughout the test period. There were no signs of gross toxicity, adverse pharmacologic effects or abnormal behavior. All animals gained weight.
- Remarks on result:
- no indication of skin sensitisation
In vivo (LLNA)
- Cellular proliferation data / Observations:
- INDUCTION PHASE :
. Test Animals: By the 4th induction many animals exhibited very mild erythema at both 24 and 48 hours post-dose. A slight increase in the severity of irritation was noted at several sites after inductions 8 and 9.
. Positive Control Animals (0.08% DNCB): Varying degrees of erythema were observed throughout induction, increasing in severity toward the end of this period.
CHALLENGE PHASE:
. Test Animals: No irritation was noted after challenge.
. Naive Control Animals: No irritation was noted after challenge.
. Positive Control Animals (0.08% DNCB): Twenty-four and 48 hours after challenge all sites were erythemic, showing a faint to moderate response. The incidence and severity of irritation.
Any other information on results incl. tables
Hours |
Sensitization Response |
|||
Incidence |
Severity |
|||
24 hours |
48 hours |
24 hours |
48 hours |
|
Test animal |
0/10 |
0/10 |
0 |
0 |
Positive control animals |
0/10 |
0/10 |
1.5 |
0.85 |
Naive Animals |
0/5 |
0/5 |
0 |
0 |
Applicant's summary and conclusion
- Interpretation of results:
- other: not sensitizing
- Conclusions:
- The chemical N,N-Diethylhydroxylamine (CAS no: 3710-84-7) was considered to be not-sensitizing to the skin of guinea pigs in a Buehler test.
- Executive summary:
A Buehler test was conducted on male guinea pigs to assess the skin sensitization potential of chemical N,N-Diethylhydroxylamine (CAS no: 3710-84-7) .
After establishing the highest non-irritating dose concentration, a 3 week induction period was initiated during which 10 young adult, male, guinea pigs were treated with the test material applied as a 30% w/w solution in deionized water and 10 were treated with 0.08% Dinitrochlorobenzene (DNCB) in 95% ethyl alcohol (positive controls). During the induction period the animals were dosed on alternate days until a total of nine dose applications were achieved. Seventeen days after the nine application a challenge dose was applied to a naive site on each guinea pig and approximately 24 and 48 hours later the animals were scored for a sensitization response (erythema and edema). A naive control group of five animals was maintained under the same environmental conditions and was treated with thewith the test material at challenge only.
All guinea pigs appeared active and healthy throughout the test period. There were no signs of gross toxicity, adverse pharmacologic effects or abnormal behavior. All animals gained weight.
After 24 and 48 hours, all sites were erythemic, showing a faint to moderate response but no irritation was observed in test and naive groups after challenge. Hence thechemicalN,N-Diethylhydroxylamine (CAS no: 3710-84-7) was considered to be not-sensitizing to the skin of guinea pigs in a Buehler test.
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