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Physical & Chemical properties

Water solubility

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Reference
Endpoint:
water solubility
Type of information:
experimental study
Adequacy of study:
key study
Study period:
experimental phase from 2014-10-06 to 2014-10-11
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
The study was conducted according to an internationally recognised method, and under GLP. No deviation was reported. The test substance is adequately characterised. Therefore full validation applies.
Qualifier:
according to
Guideline:
OECD Guideline 105 (Water Solubility)
Version / remarks:
1995
Deviations:
no
Qualifier:
according to
Guideline:
EU Method A.6 (Water Solubility)
Version / remarks:
Council Regulation (EC) No. 440/2008, Method A.6 (last modified by Reg.(EC) No.260/2014)
Deviations:
no
GLP compliance:
yes (incl. certificate)
Remarks:
inspected on June 3 to 5, 2013 / signed on November 5, 2013
Type of method:
flask method
Key result
Water solubility:
140 mg/L
Conc. based on:
test mat.
Loading of aqueous phase:
2 000 mg/L
Incubation duration:
72 h
Temp.:
20 °C
pH:
6.5
Details on results:
The analytical method was validated and tested with satisfactory results in regard to linearity, accuracy, precision and specificity:
Linearity
System quantification limit = 0.05 mg/L
Range: 0.1 – 5 mg/L with r2 = 0.9995 to 0.9999 (n=7)
Limit of Quantification
Nominal target concentration = 0.5 mg/L.
Accuracy and Precision
Recovery rates were 96 % for the 1 x LOQ (RSD=2.6%) and 103 % for the 10 x LOQ (RSD=2.6%).
Specificity
Four specific ions were detected for each signal above LOQ level, matching the spectra obtained from standards, confirming the identity for all analysed samples. Two of these ions had an m/z ratio ≥ 100 (the reference ions). The blank values of control samples showed no significant signal of the test item.

Water solubility results

No Tyndall‑effects were observed for any sample during this study.

Preincubation time

Replicate No.

Measured
pH

Water solubility
[mg/L]

Concentration (mean of two injections)

Mean

24 h

1

6.235

116

114

2

6.600

111

48 h

1

6.434

131

138

2

6.444

146

72 h

1

6.556

140

142

2

6.467

143

Mean (48 - 72 h)

 

6.5

(SD+/-0.1)

 

140

Maximum deviation [%]

 

2.4

 

Equilibrium was not considered after a pre-incubation phase of 24h, but the obtained results are homogeneous over the preincubation times of 48 and 72 h.

 

Conclusions:
Interpretation of results (migrated information): moderately soluble (100-1000 mg/L)
Executive summary:

The water solubility of the test item was determined according to OECD 105 (1995) and EC A6 (2014) guidelines, under GLP, using the flask method.

The test was conducted in double distilled water, at 20 ± 0.5 °C with preincubation at 30 ± 0.5 °C.

Equilibrium was considered achieved after a pre-incubation time of 48h.

Analytical evaluation was performed using validated GC-MS analytical method.

The water solubility was determined to be 140 mg/L (20 °C, pH 6.5)

Description of key information

Moderately soluble. Equilibrium after 48h.

pH of saturated solution: 6.5

Key value for chemical safety assessment

Water solubility:
140 mg/L
at the temperature of:
20 °C

Additional information

A fully reliable experimental study, conducted according to OECD/EC guidelines (flask method) and under GLP, is available. Therefore, it is considered as a key study, and the result is retained as key data for purpose of CSA.