Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 807-889-1
CAS number: 1255203-42-9
The study describes the ability of D-C16 to induce skin irritation on a
human three dimensional epidermal model (EPISKIN Small Model (EPISKIN-SM
TM )). The possible skin irritation potential of D-C16 was tested
through topical application for 15 minutes. The study procedures
described in this report were based on the most recent OECD and EC
Skin tissue was moistened with 5 µL of Milli-Q water and an excessive
amount of D-C16 was applied directly on top of the skin tissue for 15 ±
0.5 minutes. After a 42 hour post-incubation period, determination of
the cytotoxic (irritancy) effect was performed. Cytotoxicity is
expressed as the reduction of mitochondrial dehydrogenase activity
measured by formazan production from MTT at the end of the treatment.
Skin irritation is expressed as the remaining cell viability after
exposure to the test substance. The relative mean tissue viability
obtained after 15 ± 0.5 minutes treatment with D-C16 compared to the
negative control tissues was 106%. Since the mean relative tissue
viability for D-C16 was above 50% after 15 ± 0.5 minutes treatment D-C16
is considered to be non-irritant.
The positive control had a mean cell viability of 44% after 15 ± 0.5
minutes exposure. The absolute mean OD 570 (optical density
at 570 nm) of the negative control tissues was within the laboratory
historical control data range. The standard deviation value of the
percentage viability of three tissues treated identically with positive
control was 27%, which was due to one outlier. However, since the other
2 viabilities were clearly positive and within the historical data range
and furthermore the test substance showed a clearly negative result this
did not affect study integrity. The standard deviation value of the
percentage viability of three tissues treated identically with negative
control or test substance was less than 8%.
Finally, it is concluded that this test is valid and that D-C16 is
non-irritant in the in vitro skin irritation test under the experimental
conditions described in this report.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again