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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The test item showed 43 % and 44.4 % respiration inhibition of activated sludge at the highest test item concentration of 10000 mg/L. 
An EC50 of >10000 mg/L were obtained in this test. The effect value relates to nominal concentration, since no analytical monitoring was performed.

Key value for chemical safety assessment

EC50 for microorganisms:
10 000 mg/L

Additional information

In order to test the toxicity to microorganism of the substance, activated sludge from predominantly domestic sewage plant was exposed to the test solution of five nominal concentrations of the substance (1000, 1800, 3200, 5600 and 10000 mg/L), blank control solution and solution with positive reference substance for a period of 3 hours. The respiration rates were calculated. No physico-chemical oxygen consumption was observed at the highest concentration of test substance. The test item showed 43 % respiration inhibition of activated sludge at the highest test item concentration of 10000 mg/L,and an EC50 of >10000 mg/L were obtained.. This toxicity study is classified as acceptable and satisfies the guideline requirements for the toxicity study to microoragnisms.