Registration Dossier
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EC number: 201-052-9 | CAS number: 77-73-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Repeated dose toxicity: inhalation
Administrative data
- Endpoint:
- sub-chronic toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: animal experimental study, also published in peer-reviewed literature, minor restrictions in design and reporting but otherwise acceptable for assessment.
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 982
- Reference Type:
- publication
- Title:
- Results of a 90-day inhalation study of dicyclopentadiene in B6C3F1 mice.
- Author:
- Kransler, K. M.
- Year:
- 2 014
- Bibliographic source:
- Toxicology and Industrial Health, 30:459–466
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 413 (Subchronic Inhalation Toxicity: 90-Day Study)
- Version / remarks:
- not specified
- GLP compliance:
- no
- Remarks:
- Klimisch Cat 2
- Limit test:
- no
Test material
- Reference substance name:
- 3a,4,7,7a-tetrahydro-4,7-methanoindene
- EC Number:
- 201-052-9
- EC Name:
- 3a,4,7,7a-tetrahydro-4,7-methanoindene
- Cas Number:
- 77-73-6
- Molecular formula:
- C10H12
- IUPAC Name:
- tricyclo[5.2.1.0^{2,6}]deca-3,8-diene
- Reference substance name:
- dicyclopentadiene
- IUPAC Name:
- dicyclopentadiene
- Details on test material:
- - Name of test material (as cited in study report): dicyclopentadiene (DCPD)
- Source: Exxon Chemical Company, Baton Rouge, LA, USA
- Sample reference: BRRC 43-156
- Physical state: clear, colourless liquid
- Analytical purity: =95% endo-DCPD, 0.5% exo-DCPD
- Impurities (identity and concentrations): several impurities of which only cyclopentadiene. and isoprene were present at =0.5%
- Stability under test conditions: The composition remained stable throughout the study
Constituent 1
Constituent 2
Test animals
- Species:
- mouse
- Strain:
- other: B6C3F1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratory, Portage, MI, USA
- Age on receipt: 30-34 days
- Health assessment: confirmed following arrival
- Weight at study initiation: no data
- Housing: individually in stainless steel wire mesh suspended cages
- Diet: powdered NIH-07 diet ad libitum except during exposure
- Water: ad libitum except during exposure
- Acclimation period: no data
ENVIRONMENTAL CONDITIONS
- Temperature: 68-72°F non-exposure period
- Humidity: 40-60% non-exposure period
- Air changes (per hr): no data
- Photoperiod: 12 hrs dark / 12 hrs light
IN-LIFE DATES: July 1981 - January 1981
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: air
- Remarks on MMAD:
- MMAD / GSD: Not applicable
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Stainless steel rectangular (2mx2mx1m) exposure chamber with glass windows and door in front wall (total volume 4350 L).
- Method of holding animals in test chamber: individually in suspended stainless steel wire mesh cage with stainless steel pans between each layer of cages to prevent contamination. Cage positions were rotated routinely.
- System of generating particulates/aerosols: DCPD vapour was generated by heating the liquid in a Pyrex tube using a minimum amount of heat to prevent decomposition and formation of CPD. Filtered air was used to dilute the vapour prior to introduction into the chamber.
- Temperature and humidity in air chamber: 70-79°F, 39-68%
- Air flow rate: 2000 L/min
TEST ATMOSPHERE
- Brief description of analytical method used: Chamber concentrations were analysed at hourly intervals by gas chromatography/flame ionization detection. - Analytical verification of doses or concentrations:
- yes
- Duration of treatment / exposure:
- 13 wks
- Frequency of treatment:
- 6 hr/day, 5 days/wk
Doses / concentrationsopen allclose all
- Remarks:
- Doses / Concentrations:
0, 1, 5, 50 ppm
Basis:
nominal conc.
- Remarks:
- Doses / Concentrations:
1, 5.1, 51 ppm
Basis:
analytical conc.
- Remarks:
- Doses / Concentrations:
0, 5.4, 27.6, 276 mg/m3
Basis:
analytical conc.
- No. of animals per sex per dose:
- 45
- Control animals:
- yes
- Details on study design:
- Post-exposure observation periods of 4 and 13 weeks.
9 mice/sex/dose were scheduled for sacrifice after 2, 6 and 13 weeks of exposure and 4 and 13 weeks post-exposure.
Examinations
- Observations and examinations performed and frequency:
- CAGE SIDE OBSERVATIONS: Yes
- During exposure mice were observed several times through the chamber window.
DETAILED CLINICAL OBSERVATIONS: Yes
- Mice were observed for clinical signs before and after each exposure and daily during the recovery period.
BODY WEIGHT: Yes
- Recorded at study initiation, weekly during both the exposure period and the first 5 weeks of the recovery period, and then every 2 weeks. Animals were also weighed before termination.
FOOD CONSUMPTION: No
OPHTHALMOSCOPIC EXAMINATION: Yes
- High dose mice received ophthalmoscopic examination before sacrifice
HAEMATOLOGY: Yes
- Haematology analyses were performed on all mice prior to sacrifice after 2, 6 and 13 week exposure and 4 and 13 week post-exposure with blood from the orbital sinus. Erythrocyte count, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin and concentration, and total/differential white blood cell counts were determined.
CLINICAL CHEMISTRY: Yes
- Serum chemistry analyses were performed on all mice prior to sacrifice after 2, 6 and 13 week exposure and 4 and 13 week post-exposure with blood from the orbital sinus. Serum was analyzed for creatinine, urea nitrogen, calcium, phosphrous, chloride, alanine aminotransferase, aspartate aminotransferase, total protein, albumin, total bilirubin, alkaline phosphatase, glucose and osmolality. - Sacrifice and pathology:
- GROSS PATHOLOGY: Yes
- Necropsies were conducted on all mice.
- Kidneys, lungs, liver and testes were weighed.
- Adrenals, bone and bone marrow (sternum), brain, epididymides, eyes, heart, kidneys, larynx, liver, lungs, lymph nodes (mediastinal), muscle (gastrocnemius), nasal turbinates, parathyroids, pituitary, sciatic nerve, spleen, testes, thymus, thyroids, trachea, urinary bladder and gross lesions were preserved for microscopic evaluation.
HISTOPATHOLOGY: Yes
- Organs were examined microscopically in control and high dose mice sacrificed after 13 weeks of exposure. - Statistics:
- Analysis of variance, Bartlett’s test, Duncan’s multiple range test, F-test, Student’s t-test, Cochran t-test (applied when appropriate).
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Body weight and weight changes:
- effects observed, treatment-related
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- no effects observed
- Haematological findings:
- no effects observed
- Clinical biochemistry findings:
- no effects observed
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- no effects observed
- Gross pathological findings:
- no effects observed
- Histopathological findings: non-neoplastic:
- no effects observed
- Histopathological findings: neoplastic:
- no effects observed
- Details on results:
- CLINICAL SIGNS AND MORTALITY
- Ten males and 9 female mice exposed to 51 ppm DCPD died during the study; no more than 2 mice died at any other level.
- No significant clinical signs or body weight changes were noted prior to death. The likely cause of death appeared to be pulmonary congestion and possibly renal failure. These effects were not seen in mice sacrificed at the end of the study.
- During exposure, a few of the mice at 51 and 5.1 ppm showed coordination loss and/or decreased activity.
BODY WEIGHT AND WEIGHT GAIN
- Males and females in the 51 ppm group showed significant elevation in body wt gain that returned to parity with control values during recovery.
Effect levels
- Dose descriptor:
- NOAEC
- Effect level:
- 5 ppm (nominal)
- Sex:
- male/female
- Basis for effect level:
- other: 27.6 mg/m3. Mortality (20%) occurred in the high-dose mice during the study
Target system / organ toxicity
- Critical effects observed:
- not specified
Any other information on results incl. tables
A number of statistically significant alterations were noted in this study but the aetiology and association with dicyclopentadiene exposure are unclear. Approximately 20 percent of mice exposed to 51 ppm died during the exposure regimen. The cause of death was pulmonary congestion yet similar lung lesions were not found in animals terminated during the study. Also, female mice exposed to 51 ppm showed an increase in body weight during the last few weeks. A potential effect of dicyclopentadiene was seen in the female mice given 64 exposures to 51 or 5.1 ppm was a decrease in serum albumin indicative of slight liver dysfunction (7% difference from control); absolute and relative liver weights were also increased. No morphological changes were found to indicate any effect of dicyclopentadiene exposure. Thus any effect of dicyclopentadiene on the livers of female mice was considered to be minimal in severity.
Applicant's summary and conclusion
- Conclusions:
- Although there were no overt signs of toxicity due to dicylopentadiene, approximately 20% of mice died primarily as a result of pulmonary congestion. The aetiology and association with dicyclopentadiene exposure are unclear. The NOAEC is concluded to be 5.1 ppm (27.6 mg/m3).
- Executive summary:
Groups of 45 male and 45 female B6C3F1 mice were exposed by inhalation, 6 hr/day, 5 days/week, for 13 weeks (64 exposures) to dicyclopentadiene vapour at concentrations of 0 (air control), 1, 5.1 or 51 ppm (analysed concentrations). Animals were sacrificed after 10, 30 and 64 inhalation exposures and post-exposure sacrifices were made at 29 and 92 days following the last exposure. Clinical observations, body weights, blood clinical chemistry and haematology, ophthalmology, organ weights and histopathology evaluations were made during the study. A number of statistically significant alterations were noted in this study but the aetiology and association with dicyclopentadiene exposure are unclear. There were no overt signs of toxicity although approximately 20% of the mice of the 51 ppm exposure group died during the exposure period, primarily due to pulmonary congestion. The NOAEC is concluded to be 5.1 ppm (27.6 mg/m3).
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