N,N-dimethylformamide

Bioaccumulation: aquatic / sediment

Link to relevant study record(s)

Reference

Endpoint:

bioaccumulation in aquatic species: fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Guideline study with acceptable restrictions.

Reason / purpose for cross-reference:

reference to same study

Qualifier:

according to guideline

Guideline:

OECD Guideline 305 C (Bioaccumulation: Test for the Degree of Bioconcentration in Fish)

GLP compliance:

not specified

Radiolabelling:

no

Details on sampling:

Test fish sampling and analyses were conducted every 2 weeks, and control fish analysis before the initiation and the termination of exposure.

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

Dilution water used for the test originated from underground water pumped up from the ground of Kurume Research Laboratories.
Water quality and conditions were monitored continuously to meet the ministerial ordinance of the Ministry of Health and Welfare (1978) and the water quality criteria for fisheries (1983).

Test organisms (species):

Cyprinus carpio

Details on test organisms:

Weight, length and lipid content of the test fish at the initiation of exposure:
weight: about 30 g
length: about 10 cm
lipid content: 2-6 %

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

56 d

Total depuration duration:

28 d

Test temperature:

The temperature was 25 °C +/- 2 °C

Dissolved oxygen:

The concentration of dissolved oxygen was 6 - 8 mg/L.

Nominal and measured concentrations:

2 µg/L
20 µg/L

Reference substance (positive control):

no

Lipid content:

2 - 6 %

Time point:

start of exposure

Type:

BCF

Value:

0.3 - 1.2 L/kg

Basis:

whole body w.w.

Remarks on result:

other: Conc.in environment / dose:20 µg/L

Validity criteria fulfilled:

yes

Conclusions:

The revealed BCF range of 0.3 -1.2 L/kg indicates a very low bioaccumulation potential.

Executive summary:

An aquatic bioaccumulation test according to OECD Guideline 305C was performed with two test substance concentration levels.

The revealed BCF range of 0.3 -1.2 L/kg indicates a very low bioaccumulation potential.

Description of key information

Bioaccumulation in aquatic species: fish (OECD Guideline 305 C, GLP compliance not specified): BCF range of 0.3 -1.2 L/kg indicates a very low bioaccumulation potential.

Key value for chemical safety assessment

BCF (aquatic species):

1.2 L/kg ww

Additional information

Bioaccumulation studies on fish (Cyprinus carpio) showed no significant accumulation potential (BCF = 0.3 - 1.2 L/kg after 56 days). Additionally, the experimentally determined log Kow = -0.85 (BASF, 1987) also demonstrates a low potential for bioaccumulation.