Hydrazine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: description sufferes from deficiencies because no individual animal data were given.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

The acute toxicity of the vapours of some methylated hydrazine derivatives

Author:

Jacobson KH, Clem JH, Wheelwright HJ, Rinehart WE, Mayes N

Year:

1955

Bibliographic source:

AMA Arch. Ind. Health 12, 609-616

Reference 2

Reference Type:

publication

Title:

The Toxicology of an Aniline-Furfuryl Alcohol-Hydrazine Vapor Mixture.

Author:

Jacobson KH, Rinehart WE, Wheelwright jr HJ, Ross MA, Papin JL, Daly R, Greene EA, Groff WA

Year:

1958

Bibliographic source:

J. Am. Ind. Hyg. Assoc. 19, 91-100.

Materials and methods

Principles of method if other than guideline:

Method: 5 concentrations tested; 10 animals per group; 14 days post exposure observation period.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Hydrazine

EC Number:

206-114-9

EC Name:

Hydrazine

Cas Number:

302-01-2

Molecular formula:

H4N2

IUPAC Name:

hydrazine

Details on test material:

IUCLID4 Test substance: other TS: hydrazine, no data on purity; M.P. 1.4 °C

Test animals

Species:

rat

Strain:

not specified

Sex:

male

Details on test animals or test system and environmental conditions:

no further data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

other: other

Details on inhalation exposure:

Chamber concentration were established by passing dry nitrogen through a bubbbler containing the liquid agent.The vapour was mixed with air in a glass premixing bowl, and this mixture was then drawn into a constant flow gassing chamber. A 0.4m³ chamber operated at an air flow of 0.10 to 0.115 m³ per minute. Samples of chamber air were drawn into Vigreaux-type bubblers containing water acidified with a drop of concentrated hydrochloric acid. Analyses of hydrazine was performed by potentiometric bromate titration.

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

400 - 800 ppm

No. of animals per sex per dose:

10

Control animals:

not specified

Details on study design:

5 concentrations tested;
10 animals per group;
14 days post exposure observation period;
one group of rats at each concentration was killed periodically
after exposure for a study of pathological changes. The other
group was observed for development of toxic signs during
exposure and for 14 days thereafter.

Statistics:

method of Bliss as described by Finney

Results and discussion

Effect levels

Key result

Sex:

male

Dose descriptor:

LC50

Effect level:

570 ppm

95% CL:

504 - 649

Exp. duration:

4 h

Remarks on result:

other: corresponding to 759 mg/m³

Mortality:

Individual data are not given, only a graphic: range between 20 and 90 %.

Clinical signs:

other: Restlessness, dyspnea, clonic convulsions with exophthalmos.

Body weight:

no data

Gross pathology:

no data

Other findings:

no data

Any other information on results incl. tables

LC50: 0.759 mg/l; 95% confidence limits 504-649 ppm.
Clinical signs: restlessness, dyspnea, convulsions.

Applicant's summary and conclusion

Interpretation of results:

Category 2 based on GHS criteria

Executive summary:

10 male rats/group were exposed to 400 -800 ppm for 4 hours and displayed restlessness, dyspnea and convulsions, and resulted in a LC 50 (male rat) of 570 ppm (759 mg/m³, Jacobson 1955).