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EC number: 231-150-7
CAS number: 7440-41-7
Preparing extracts was the only relevant method to test beryllium metal
in vitro, because the test item is barely soluble in (aqueous) cell
culture media. Extraction was conducted at 37 °C in the dark under
non-abrasive shaking, simulating human lung conditions. Three days were
choosen as extraction period, to have the best compromise (highest
possible beryllium-ion-concentration while keeping essential cell
culture ingredients like vitamins etc. intact).
The Ames-test, the chromosome aberration test in human lymphocytes and
the mammalian cell gene mutation assy (HPRT) were negative both in
presence and absence of metabolic activation system (rat liver S9). The
unscheduled DNA repair assay did not reveal a general DNA damaging
potential of the beryllium metal extract. There was a reduction in the
mean net grain count (reflecting active DNA repair) when beryllium
extract was incubated in parallel with the mutagen 2
-acetylaminofluorene. However, there was no clear dose-response for this
effect. The Syrian hamster cell transformation test indicated a cell
transforming potential. The relevance of this finding in absence of any
effects on DNA damage, cytogenicity and gene mutation remains unclear.
No genotoxicity was observed in the classical investigations in vitro.
The observed effect on cell transformation remains unclear, but in
absence of a DNA damaging potential/ absence of a potential to inhibit
DNA repair, this is not considered to reflect a classical genotoxic
action. Consequently, no classification is applied.
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