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EC number: 290-754-9
CAS number: 90218-76-1
All oxygen concentrations are given in mg O2 /
The study of the bacteriotoxicity of 1,2,4-Benzenetricarboxylic
acid, mixed decyl and octyl triesters was carried out by a method
developed by the testing laboratory. Principle of the method: 100 mL BOD
bottles closable by means of ground stoppers are to be charged with the
culture solution, the suspension of bacteria (Pseudomonas putida Migula)
and the test substance in stepped concentrations, closed so as to leave
no air bubbles and incubated for 5 -6 hours at 25 ± 2 °C in a shaking
incubator. Batches free of test substance serve as control. The
difference between the oxygen content of the solutions in the individual
containers at time zero and after the incubation time gives the
bacterial oxygen consumption. Comparison of the amounts of oxygen
consumed in the control and test batches gives information about the
concentration-dependent influence of the test substance on the oxygen
For the test 2.0 g of the test substance was weighed in
20 mL neutralised emulsifier (nonylphenol 10 EO, 5 PO) and emulsified in
a shaking incubator at 25 °C for 19 h at 200 rpm. 1 mL of this test
solution was given into the test vessel (100 mL) for the highest test
concentration (1000 mg/L, corresponding to 980 mg a.i./L). For further
test concentrations the test solution was adequately diluted with
emulsifying solution (dilution 1:10 each).
The test substance did not affect the bacterial
respiration in the tested concentration range (1, 10, 100 and 1000 mg/L;
corresponding to 1, 9.8, 98 and 980 mg a.i./L),:
EC10 > 0.98 g a.i./L, EC50 > 0.98 g a.i./L.
The test substance was not toxic under test conditions in the range of water solubility.EC10 > 0.98 g a.i./L, EC50 > 0.98 g a.i./L.
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