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Short-term toxicity to fish

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Description of key information

With high probability acutely not harmful to fish

Key value for chemical safety assessment

Additional information

Two acute fish studies were performed according to German Industrial Standards DIN 38412, part 15 using Leuciscus idus as test species (BASF AG, 1990, report no. 10F0990/885214; BASF AG, 1987, report no. 10F0333/875143). The results indicate that the test substance is probably acutely not harmful to fish. The 96-hour LC50 was determined to be >1000 and <2200 mg/L for not neutralized test solutions. In pelagic toxicity testing a pH of 6.5 to 9.0 is generally accepted (REACH Guidance, R.7B). This value was exceeded in the initial phase of all test item solutions. The pH remained above the critical value of pH 9.0 in the test item solutions of >= 2150 mg/L, which could have caused the high mortality in these test solutions. It should be noted that the mortality was not reduced after pH-adjustment of the highest concentration level (10000 mg/l). However, mortality was delayed. The observed mortalities were at least partly due to the pH-shift in the higher test item concentrations.

An additional acute toxicity study using juvenile sheepshead minnows in a semi-static approach resulted in a 96-h LC50 of >1000 mg/L, therefore demonstrating comparable endpoints between freshwater and marine fish species. The test solutions were not neutralised prior to use (BASF AG, 2002; report no. V2489/03).