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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Activated sludge from a laboratory wastewater treatment plants fed with municipal and synthetic sewage
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
28 d
Initial conc.:
20 mg/L
Based on:
DOC
Initial conc.:
109 mg/L
Based on:
test mat.
Details on study design:
TEST CONDITIONS
- Composition of medium: 500 mL destilled water, 13 mL nutrient solution, 5 mL inoculum, 51 mL stock solution test substance, filled up with distilled water to a total volume of 1000 mL.

TEST SYSTEM
- Culturing apparatus: Shimadzu TOC 500
- Number of culture flasks/concentration: 2

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: yes
- Toxicity control: yes
Reference substance:
benzoic acid, sodium salt
Key result
Parameter:
% degradation (DOC removal)
Value:
>= 70 - <= 80
Sampling time:
28 d
Interpretation of results:
readily biodegradable, but failing 10-day window

Description of key information

In a study according to OECD guideline 301, the test substance was readily biodegradable, but failed the 10-d window.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable but failing 10-day window
Type of water:
freshwater

Additional information