2-imidazolidone

Specific investigations: other studies

Administrative data

Endpoint:

cytotoxicity

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given, acceptable for assessment.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1988

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Cell cultures of foetal rat-brain tissue were incubated with the chemical at 0.5, 1 and 2 mM (1 mM = 86 µg/mL) for 3 days. Cytotoxicity to neuronal and non-neuronal cells was assayed by qualitively evaluating the morphological integrity of the monolayer (e.g. cell death, depressed sprouting of neurites).

GLP compliance:

not specified

Type of method:

in vitro

Endpoint addressed:

developmental toxicity / teratogenicity

Test material

Test material information

Constituent 1

Reference substance name:

2-imidazolidone

EC Number:

204-436-4

EC Name:

2-imidazolidone

Cas Number:

120-93-4

Molecular formula:

C3H6N2O

IUPAC Name:

2-imidazolidone

Test material form:

solid

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Charles River, St. Constant, Quebec

Administration / exposure

Route of administration:

other: in vitro exposure of primary monolayers cultured from dissociated foetal rat brains

Vehicle:

other: nutrient medium

Duration of treatment / exposure:

72 h

Frequency of treatment:

continuous

Doses / concentrations

Doses / concentrations 1

Dose / conc.:

0.5 other: mM (nominal)

Doses / concentrations 2

Dose / conc.:

1 other: mM (nominal)

Doses / concentrations 3

Dose / conc.:

2 other: mM (nominal)

Examinations

Examinations:

Exposed cultures were examined daily for degenerative changes progressively disrupting the monolayer.

Results and discussion

Details on results:

No degenerative effect on both, neuronal and non-neuronal foetal rat-brain cells in vitro.
The idea behind this assay was testing for "neuroteratogenicpotential", primary cell cultures from foetal brain serving as surrogate of the initial targetorgans, peripheral and central nervous system.