Registration Dossier

Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
1964-06-16 to 1964-06-29
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Non-standard study. Non-GLP study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1964
Report Date:
1964

Materials and methods

Principles of method if other than guideline:
BASF-test
GLP compliance:
no
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
other: colorless liquid

Test animals

Species:
mouse
Strain:
other: Hannover
Sex:
male/female
Details on test animals and environmental conditions:
TEST ANIMALS
- Age: young adult

Administration / exposure

Route of administration:
intraperitoneal
Vehicle:
other: aqueous emulsion with tragacanth
Details on exposure:
Form of administration: 1 - 2% aqueous emulsion with tragacanth
Route of aciministration: Intraperitoneal
Doses:
1600, 200, 160, 125, 100, 50 mg/kg bw
No. of animals per sex per dose:
10 (5 males and 5 females) per dose
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations: daily
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, histopathology

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 0.11 mL/kg bw
Based on:
test mat.
Remarks on result:
other: corresponding to a LD50 of 98 mg/kg bw assuming a density of 0.89 g/mL of the test item
Mortality:
After 14 days observation period, the dead rate was as follows:
1600 mg/kg bw: 10/10
200 mg/kg bw: 7/10
160 mg/kg bw: 8/10
125 mg/kg bw: 7/10
100 mg/kg bw: 1/10
50 mg/kg bw: 2/10
Clinical signs:
Staggering, dyspnea and narcosis; convulsions and late deaths
Body weight:
No data
Gross pathology:
No abnormalities detected
Other findings:
No data

Applicant's summary and conclusion