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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1972
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions
Remarks:
Only mortality figures are given. No additional adverse effects are reported; therefore this study can be considered as reliable with restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1972
Report Date:
1972

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
Deviations:
not specified
GLP compliance:
not specified
Test type:
acute toxic class method

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
rat
Strain:
Sprague-Dawley
Remarks:
200-300 gram
Sex:
male
Details on test animals and environmental conditions:
Also used 20-30 gram male CF1 (ICR derived) mice

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
corn oil
Details on oral exposure:
Glass syringes with special needles were used to intubate all doses. The experimental animals were fasted for at least 16 hours prior to administration of the oral dose and were not given food for at least 2 hours after injection. This was to allow for more uniform absorption in all animals of the same species, since the amount of food in the stomach varies from animal to animal in the unfasted condition.
The intubation volume for both rats and mice was approximately 0.01 ml/gm body weight which meant that the average mouse received a volume of 0.25 ml, and the rat 2. 5 ml. The concentrations of the solutions or suspensions were adjusted to give the proper dose in the desired volume
Doses:
Initial rangefinding tests were conducted on each compound at the following dose levels: 5 mg/kg, 50 mg/kg, and 500 mg/kg. After the proper range had been found, subsequent doses were administered according to either a multiple of 2 or 1. 26 of the starting dose.
No. of animals per sex per dose:
5 rats and 5 mice were tested at each level chosen
Control animals:
not specified

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
LD50
Effect level:
ca. 2 462 mg/kg bw
Based on:
test mat.
95% CL:
> 1 788 - < 3 389
Mortality:
Data used to calculate LD50 in mg/kg bw (Mortality Response, N=5)
1000 (0) ; 2000 (1) ; 4000 (5)

Applicant's summary and conclusion

Interpretation of results:
Category 5 based on GHS criteria
Conclusions:
The authors estimated the single oral dose toxicity of benzal chloride (CAS n° 98 -87-3) in male Sprague-dawley rats and in male CF-1 mice.
In the test conditions, the authors found a LD50 of 3250 and 2462 mg/kg bw respectively in male rats and mice. Therefore the substance is put in category 5 regarding the GHS criteria
Only mortality figures are given. No additional adverse effects are reported; therefore this study can be considered as reliable with restrictions.
Executive summary:

The authors estimated the single oral dose toxicity of benzal chloride (CAS n° 98 -87-3) in male Sprague-dawley rats and in male CF-1 mice.

In the test conditions, the authors found a LD50 of 3250 and 2462 mg/kg bw respectively in male rats and mice. Therefore the substance is put in category 5 regarding the GHS criteria

Only mortality figures are given. No additional adverse effects are reported; therefore this study can be considered as reliable with restrictions.