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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Description of key information

No toxic effects up to the limit of water solubility (< 0.05 mg/L) for Pseudomonas putida based on read-across 

Key value for chemical safety assessment

Additional information

There are no adequate study data on toxicity to microorganisms of the registration substance available. The assessment was therefore based on studies conducted with the analogue substance 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters (CAS No. 90218-76-1) as part of a read across approach, which is in accordance with Regulation (EC) No. 1907/2006. Annex XI, 1.5. For each specific endpoint the source substance(s) structurally closest to the target substance is/are chosen for read-across, with due regard to the requirements of adequacy of the available data. Structural similarities and similarities in properties and/or activities of the source and target substance are the basis of read-across. A detailed justification is provided in the technical dossier (see IUCLID Section 13). Please consider that the originally proposed category approach addressing all Polyfunctional acid esters (PFAE) aromatic is currently under revision and will finally result in an analogue approach for the registration. Therefore, two documents, the category justification and explanatory notes for the analogue approach are provided in Section 13.


The study with 1,2,4-Benzenetricarboxylic acid, mixed decyl and octyl triesters (CAS No. 90218-76-1) was performed under GLP but did not follow a currently standardized guideline. However, the general test performance is suitable to determine effect concentrations as required for this endpoint. The effect of the test substance on the oxygen consumption of Pseudomonas putida was tested under static conditions using nominal concentrations in the range of 1 to 1000 mg/L, prepared using an emulsifier. A EC10 (4.75 h) > 1000 mg/L was determined on the basis of the nominal test concentration.


Thus, based on the above mentioned results, and due to the structural similarities and a consistent ecotoxicological/environmental fate profile of the substances, it can be concluded that no toxicity of 1,2,4-Benzenetricarboxylic acid, tri-C9-11-alkyl esters (CAS No CAS No. 94279-36-4) to microorganisms is expected.