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Toxicity to soil microorganisms

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The adverse effects of Bronopol on aerobic soil microorganisms were investigated by means of the nitrate production test according to OECD guideline 216 with Lucerne meal as nitrogen source. The content of nitrate in aqueous soil extracts was determined by means of ion chromatography using an IC system Dionex DX 120 apparatus. The percentage of inhibition of the nitrate production in the treated soil samples versus untreated controls was calculated.The EC10 on nitrogen transformation after 28 days exposure to Bronopol was 25 mg/kg dry matter soil; the corresponding EC50 after 28 days = 679 mg/kg dry matter soil.

A second study for the same purpose has been conducted by means of a carbon transformation test according to OECD guideline 217, which measures the carbon transformation by the glucose induced soil respiration. The respiration rates were measured for 12 consecutive hours in respiration measurement units (OxiTop) by measuring the negative pressure resulting from absorbed CO2 produced by glucose. The EC10 on carbon transformation after 28 days exposure to Bronopol was determined to be 70 mg/kg dry matter soil; the corresponding EC50 after 28 days was > 1186 mg/kg dry matter soil.