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EC number: 814-072-3 | CAS number: 77614-79-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March 2019 - July 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 019
- Report date:
- 2019
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
- Version / remarks:
- 2000
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 3,5-bis(difluoromethyl)-1H-pyrazole
- EC Number:
- 814-072-3
- Cas Number:
- 77614-79-0
- Molecular formula:
- C5H4F4N2
- IUPAC Name:
- 3,5-bis(difluoromethyl)-1H-pyrazole
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
- Sampling method: 10 g soil d.w. per replicate at 3 h, 7 d, 14 d and 28 d after application
- Sample storage conditions before analysis: Soil extracts stored deepfrozen prior to analysis at -20 5 °C
Test substrate
- Vehicle:
- yes
- Remarks:
- Quartz sand
- Details on preparation and application of test substrate:
- AMENDMENT OF SOIL
- Type of organic substrate: lucerne meal (C/N ration = 13.2 /1)
APPLICATION OF TEST SUBSTANCE TO SOIL
- Method: Mixture of test substance with quartz sand. Mixture of the spiked sand and soil using a hand stirrer.
VEHICLE:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Quartz sand
- Concentration of vehicle in test medium (stock solution and final test solution): 10 g / kg soil d.w.
Test organisms
- Test organisms (inoculum):
- soil
Study design
- Total exposure duration:
- 28 d
Test conditions
- Test temperature:
- 19.5 - 20.7 °C
- Moisture:
- 15.94 - 16.78 g /100g soil d.w. equivalent to 45.77 - 48.17 % of WHC
- Organic carbon content (% dry weight):
- 1.47
- Nitrogen content (% dry weight):
- 0.14
- Details on test conditions:
- TEST SYSTEM
- Amount of soil: 200 soil d.w.
- No. of replicates per concentration: 3
- No. of replicates per control: 3
SOIL INCUBATION
- Method: series of individual subsamples
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographical reference of sampling site (latitude, longitude): Saxony, Germany ,12.694435960 degrees East, 51.403774567 degrees North
- Vegetation cover: fallow ground
- Treatments with fertilizers: not sinze 203
- Treatments with pesticides: not since 1990
- Depth of sampling: 20 cm
- Soil texture according to USDA
- % sand: 21.4
- % silt: 38.3
- % clay: 10.3
- Soil taxonomic classification: loam
- Soil classification system: USDA
- pH (in water): 6.2
- Maximum water holding capacity (in % dry weigth):34.83
- Cation exchange capacity (mmol/kg): 9.9
- Pretreatment of soil: drying and sieving (2mm) at room temperature
- Storage (condition, duration): 1.5 month at 4 °C
- Initial microbial biomass as % of total organic C: 2.44 %
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
Soil samples (10 g soil d.w. per replicate) were taken at intervals of 3 hours, 7, 14 and 28 days after application and the NH4-N-, NO3-N- and NO2-N-contents were determined - Nominal and measured concentrations:
- 0.01 and 0.1 mg test item / kg soil dry weight corresponding to 0.005 and 0.05 kg test item/ha
- Reference substance (positive control):
- yes
- Remarks:
- Dinoterb as internal standart for validation of test system (not inculded in guideline requirements)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 28 d
- Dose descriptor:
- other: > 25 % Difference to control
- Effect conc.:
- > 0.1 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Duration:
- 28 d
- Dose descriptor:
- other: > 25 % Difference to control
- Effect conc.:
- > 0.01 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- nitrate formation rate
- Details on results:
- Differences from the control of +23.3 % (test concentration 0.01 mg test substance/kg soil dry weight) and +1.4 % (test concentration 0.1 mg test substance /kg soil dry weight) were measured at the end of the 28-day incubation period (time interval 14-28).
Any other information on results incl. tables
Table 1: Effects on nitrogen transformation in soil after treatment
Time |
Control |
0.01 mg pure metabolite/kg |
0.1 mg pure metabolite/kg |
||
|
Nitrate-N1) |
Nitrate-N1) |
% difference |
Nitrate-N1) |
% difference |
0-7 |
3.66 ± 0.2 |
3.64 ± 0.1 |
-0.5n.s. |
3.92 ± 0.52 |
+7.2n.s. |
7-14 |
1.16 ± 0.16 |
0.6 ± 0.15 |
-47.7*s. |
0.81 ± 0.15 |
-29.6n.s. |
14-28 |
1.05 ± 0.21 |
1.3 ± 0.04 |
+23.3n.s. |
1.07 ± 0.24 |
+1.4n.s. |
1) Rate: Nitrate-N in mg/kg soil dry weight/time interval/day, mean of 3 replicates and standard deviation
n.s. = No statistically significant difference to the control (Student-t-test for homogeneous variances, 2-sided, p > 0.05)
*s. = statistically significantly different to control (Student-t-test for homogeneous variances, 2-sided, p ≤ 0.05)
Table 2: Validity criteria for OECD 216.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
The variation between replicate control samples should be less than ± 15%. |
6.1 % |
Yes |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
- Conclusions:
- The test item caused no adverse effects (difference to control < 25 %, OECD 216) on the soil nitrogen transformation (expressed as NO3-N-production) at the end of the 28-day incubation period.
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