Butanenitrile, 2-amino-2,3-dimethyl-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1984

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: USEPA 660/3-75009

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-amino-2,3-dimethylbutanenitrile

Cas Number:

13893-53-3

Molecular formula:

C6H12N2

IUPAC Name:

2-amino-2,3-dimethylbutanenitrile

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

no

Details on test solutions:

- Test concentrations were prepared by preparing a stock solution in deionized water and serially diluting to obtain desired concentrations.

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 +/- 2°C

Nominal and measured concentrations:

- The preliminary test concentrations were set at 0.1, 1.0, and 10 mg/L.
- Based on the results of the preliminary testing, five test concentrations (nominal) were selected and tested in duplicate, 0 (control), 0.56, 1.0, 1.8, 3.2, 5.6, and 10 mg/L.

Details on test conditions:

- The static Daphnia magna bioassay was conducted in 250 ml glass beakers, 10 daphnids/ beaker, containing 200 ml of ABC well water.
- These vessels were kept at 20 (± 2) °C.
- The lighting was maintained at 50-70 foot-candles on a 16 hour daylight photoperiod.
- An initial range-finding test was conducted to determine the concentration range for the definitive study.
- All results were based on the nominal concentrations.
- Water quality parameters of temperature, dissolved oxygen and pH were measured at the termination of the test and were within acceptable limits.
- The dissolved oxygen concentrations, which ranged between 8.4 and 8.8 mg/l, were considered adequate for testing.
- The pH values of the treated chambers were consistent with the control and ranged form 8.2 to 8.7.
- The no-effect concentration based on the lack of mortality and abnormal effects was 3.2 mg/l.
- The abnormal effects of mortality and/or daphnids lying on the bottom were observed after 24 and 48 hours of exposure in the 5.6 mg/l (24 hr-2/20 dead and 48-hr-3/20 dead) and 10 mg/l (24 hr-15/20 and 48-hr-20/20 dead) test concentrations.
- Statistical analysis of the concentration vs. effect data was obtained by employing a computerized LC50 program.
- This program calculated the LC50 statistic and its 95% C.L. using the binomial and the moving average tests.
- The method of calculation selected for use was that which gave the narrowest confidence limits for the LC50.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

3.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

6.9 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility