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EC number: 228-589-1 | CAS number: 6300-50-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 1984
- Qualifier:
- according to guideline
- Guideline:
- other: Real Decreto 363/1995 (BOE núm. 133 de Junio de 1995)
- GLP compliance:
- no
- Analytical monitoring:
- no
- Vehicle:
- yes
- Details on test solutions:
- Taking into account the value of 20% of active ingredient, the study will be carried out with the 100% active ingredient of the presented material.
Preparation of the 1 g / L of the test material : 1 g of the test material has been dissolved in 100 mL of culture medium in a volumetric flask, Class A of 100 mL by means of agitation and a stock solution of 1000 mg / L has been obtained. The test material has a content of 20% active material, thus the stock solution contains 200 mg / L of active material. The different solutions used in the test have been prepared from this stock solution of 1000 mg / L.
Actual concentration of the stock solution: 1007 mg/L of active ingredient. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Age: ≤ 24-hours life, in good physiological conditions
Origin: Stirling University, United Kingdom
Culture conditions: The cultivation of daphnia from which the Individuals to carry out the test has been done with the following conditions:
- Temperature: between 18 and 22 ºC
- Photoperiod of 16 hours of light / 8 hours of darkness
- No aeration
- Feeding: Type of food: Chlorella (freshwater microalgae) and marine algae extract
·-Frequency of feeding: 3 times / week - Test type:
- not specified
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 ± 2 ºC
- pH:
- average value pH at the beginning of the test: 7,62 (interval 7,66-7,81)
average value pH at the end of the test: 7,39 (interval 7,42-7,76) - Dissolved oxygen:
- average value O2 at the beginning of the test: 8,52 mg/L (interval 8,52-8,49 mg/L)
average value O2 at the end of the test: 8,41 mg/L (interval 8,39-8,3 - Nominal and measured concentrations:
- Nominal: 1 mg/L, 2,2 mg/L, 4,8 mg/L, 10 mg/L, 22 mg/L, 48 mg/L, 100 mg/L, 220 mg/L, 480 mg/L and 1000 mg/L
Measured: 1,01 mg/L, 2,22 mg/L, 4,83 mg/L, 10,07 mg/L, 22,15 mg/L, 48,35 mg/L, 100,7 mg/L, 221,5 mg/L, 483,4 mg/L and 1007 mg/L. - Details on test conditions:
- Photoperiod: darkness
Test vessels: Test tubes (40 mL) capped with parafilm.
Test volume used: 10 mL
Number of test vessels per concentration: 4
Number of individuals per concentration: 20, divided into 5 individuals per test vessel
Age of individuals: less than 24 hours of life
Composition of culture medium OECD 202:
- Solution 1: CaCl 2 2H 2 O 11.76 g / L H 2 O
- Solution 2: MgSO 4 7H 2 O 4.93 g / L H 2 O
- Solution 3: NaHCO 3 2.59 g / L H 2 O
- Solution 4: KCl 0.23 g / L H 2 O
Add to 1000 mL of deionized water:
Solution 1 25 mL
Solution 2 25 mL
Solution 3 25 mL
Solution 4 25 mL - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 200 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- The EC50-24h of potassium dichromate is between 0.6 mg / L and 1.7 mg / L
- Validity criteria fulfilled:
- yes
- Conclusions:
- The result obtained in this test indicates that the test substance is not toxic to Daphnia magna at the concentration of 200 mg / L of active material.
Reference
Description of key information
There is one study available to determine the short-term toxicity of the substance Direct Red 254 sodium salt (CAS 6300 -50 -1) to Daphnia according to OECD Guideline 202. At the maximum concentration tested (EC50(daphnia)>200 mg/L act. ingr.) and considering the existing information, the substance is not harmful to aquatic invertebrates.
There are two additional studies conducted on the structural analogue substance Direct Red 254 TEA salt (CAS 64683-40 -5). The EC50 (48h) values are 155 mg/L active ingr. and 175 mg/L active ingr. respectively, show that the tested substance is not harmful to aquatic invertebrates.
The tested substances have an identical structure in respect to the anionic constituents.
The results of all the studies support the effect of the substance to aquatic invertebrates and the read-across approach between both structures.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 200 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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