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EC number: 224-923-5 | CAS number: 4553-62-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Repeated dose toxicity by inhalation route: NOAEC is greater than 200 mg/m3.
Decrease of the Body weight gain was observed at 200 mg/m3 compared to control groups. The Food consumption was not examined.
Moreover, the body weight gain was normal again and similar to the control group during the recovery period. Therefore, this decrease of body weight gain can not be considered as an adverse effect.
A increased of reticulocytes was also observed, however this reticulocyte increase was not relevant in the face of normal RBC mass and thus the changes in reticulocyte values were not considered to be adverse.
Key value for chemical safety assessment
Repeated dose toxicity: inhalation - systemic effects
Endpoint conclusion
- Dose descriptor:
- NOAEC
- 200 mg/m³
- Study duration:
- subacute
- Species:
- rat
Additional information
Repeated dose toxicity by oral route:
No data available.
Repeated dose toxicity by dermal route:
No data available
Repeated dose toxicity by inhalation route:
One repeated dose toxicity study by inhalation route was available and was considered as a key study (Kelly, 2001).
In a subchronic inhalation toxicity study performed according to OECD 412 guideline, 2 -methylglutaronitrile (99.6%) was administered to 20 male Crl:CD (SD) IGS BR rats/concentration by nose only exposure at concentrations of 0, 5, 25, and 200 mg/m3(0, 0.005, 0.025 and 0.2 mg/L) for 6 hours per day, 5 days/week for a total of 20 days.
No effect was observed for all parameters examined except a slight/transient effect on body weight gain and a statistically significant
increased of reticulocytes. Indeed, at the highest concentration tested (200 mg/m3), the body weight gain was significantly lower in this group of rats (for a period between Day 15 and Day 26) than in the control group. The body weight gain was normal again and similar to the control group during the recovery period. As there is no information on the food consumption, no relevant link can be established between this depressed body weight gain and the Substance tested. Furthermore, this depressed body weight gain can not be considered as an adverse effect, because the rats exposed to the highest concentration did not present a true loss of weight. They continued to gain weight but in a lower extent in comparison to the control group. Moreover, reticulocyte increase was not relevant in the face of normal RBC mass and thus the changes in reticulocyte values were not considered to be adverse.
Therefore, based on these results, the NOAEC is greater than 200 mg/m3(0.2 mg/L) and the NOEC is 25 mg/m3(0.025 mg/L) for body weight gain reduction and increased of reticulocytes.
Justification for classification or non-classification
2 -methylglutaronitrile is not classified in the Annex VI of the CLP regulation (1272/2008).
Repeated dose toxicity by inhalation route/self classification:
Based on the key study, the NOAEC is greater than 200 mg/m3(0.2 mg/L) and the NOEC is 25 mg/m3(0.025 mg/L) for a decrease on the Body weight gain at 200 mg/m3 compared to control groups and for a increased of reticulocytes.
Based on these results, no classification is required according to the CLP regulation (1272/2008) and to the Directive 67/548/CEE.
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