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Diss Factsheets
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EC number: 201-108-2 | CAS number: 78-35-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
![](https://echa.europa.eu/o/diss-blank-theme/images/factsheets/A-REACH/factsheet/print_ecotoxicological-information.png)
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study, to GLP.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 015
- Report date:
- 2015
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,5-dimethyl-1-vinylhex-4-enyl isobutyrate
- EC Number:
- 201-108-2
- EC Name:
- 1,5-dimethyl-1-vinylhex-4-enyl isobutyrate
- Cas Number:
- 78-35-3
- Molecular formula:
- C14H24O2
- IUPAC Name:
- 1,5-dimethyl-1-vinylhex-4-enyl isobutyrate
- Reference substance name:
- Unknown impurities
- Molecular formula:
- not applicable
- IUPAC Name:
- Unknown impurities
- Test material form:
- liquid
- Details on test material:
- Identification: Linalyl isobutyrate
Chemical name: 3,7-dimethyl-1,6-octadien-3-yl 2-methylpropanoate
Empirical Formula: C14H24O2
Molecular Weight: 224.35 g/mol
EC No.: 201-108-2
CAS No.: 78-35-3
Physical state/Appearance: Clear colorless liquid
Constituent 1
impurity 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- At 0 and 24 hours for fresh media and at 24 and 48 hours for old media.
Test solutions
- Details on test solutions:
- In the range-finding test Daphnia magna were exposed to a series of nominal test concentrations of 0.10, 1.0, 10 and 100% v/v saturated solution.
Based on the results of the range-finding test the following test concentrations were assigned to the definitive test: 10, 18, 32, 56 and 100% v/v saturated solution.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- The test was carried out using 1st instar Daphnia magna derived from in-house laboratory cultures.
Adult daphnia were maintained in 150 mL glass beakers containing Elendt M7 medium (see Appendix 2) in a temperature controlled room at approximately 20 °C. The lighting cycle was controlled to give a 16 hours light and 8 hours darkness cycle with 20 minute dawn and dusk transition periods. Each culture was fed daily with a mixture of algal suspension (Desmodesmus subspicatus) and Tetramin® flake food suspension. Culture conditions ensured that reproduction was by parthenogenesis. Gravid adults were isolated the day before initiation of the test, such that the young daphnids produced overnight were less than 24 hours old. These young were removed from the cultures and used for testing. The diet and diluent water are considered not to contain any contaminant that would affect the integrity or outcome of the study.
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- none
Test conditions
- Test temperature:
- Temperature was maintained at 19 to 20 °C throughout the test. The water temperature was recorded daily throughout the test using a Hanna Instruments HI 93510 digital thermometer.
- pH:
- The pH of the prepared media was 7.9 ± 0.3. The pH was measured daily using a Hach Flexi handheld meter
- Dissolved oxygen:
- The dissolved oxygen concentration at the end of the test is ≥3 mg/L in the control and test vessels. The dissolved oxygen concentration was measured daily using a Hach Flexi handheld meter
- Salinity:
- No (freshwater)
- Nominal and measured concentrations:
- Range finding test: Nominal test concentrations of 0.10, 1.0, 10 and 100% v/v saturated solution
Definitive test: Nominal test concentrations of 10, 18, 32, 56 and 100% v/v saturated solution; 0-Hour measured test concentrations: 0.35, 0.66, 1.3, 2.2, 4.3 mg/L
- - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 1.4 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% CL: 1.2 - 1.7
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.66 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- LOEC
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- In accordance with the tiered approach for selecting an appropriate exposure regime for unstable and volatile substances (OECD 2000), a closed semi-static system was used with no headspace. Under such conditions it is considered appropriate to base the effect results on the mean measured concentrations of fresh solutions. This gave a 48-hour EC50 of 1.4mg/L for linalyl isobutyrate. In order to give a “worse case” analysis of the data, the results were also calculated using the time-weighted mean measured test concentrations, which gave a slightly lower 48-hour EC50 value of 1.1 mg/L. Both results are within the same environmental classification band of > 1 and <= 10 mg/L.
- Results with reference substance (positive control):
- A positive control used potassium dichromate as the reference item at concentrations of 0.32, 0.56, 1.0, 1.8 and 3.2 mg/L.
Exposure conditions for the positive control were similar to those in the definitive test.
Analysis of the immobilization data by probit analysis using linear maximum likelihood regression at 24 and 48 hours. All analysis was carried out using the ToxRat Professional computer software package (ToxRat):
The 24 h EC50 was 1.1 mg/L .
The 48 h EC50 was 0.75 with 95 % confidence limits of 0.69 - 0.82 mg/L.
The NOEC was 0.56 mg/L and the LOEC was 1.0 mg/L.
The No Observed Effect Concentration is based upon less than 10% immobilization at this concentration.
The results from the positive control with potassium dichromate were within the normal range for this reference item.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In a guideline study, conducted according to GLP, Linalyl Isobutyrate was found to have an EC50 (48 hr) (average initial measured test concentrations) of 1.4 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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