Adenosine phosphate

Melting point / freezing point

Administrative data

Endpoint:

melting point/freezing point

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 March - 01 June 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2017

Report date:

2017

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method A.1 (Melting / Freezing Temperature)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 102 (Melting point / Melting Range)

Version / remarks:

from 27 July 1995

Deviations:

no

Test guideline 3

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7200 (Melting Point / Melting Range)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

differential scanning calorimetry

Test material

Test material information

Constituent 1

Reference substance name:

Adenosine phosphate

EC Number:

200-500-0

EC Name:

Adenosine phosphate

Cas Number:

61-19-8

Molecular formula:

C10H14N5O7P

IUPAC Name:

adenosine phosphate

impurity 1

Reference substance name:

Water

EC Number:

231-791-2

EC Name:

Water

Cas Number:

7732-18-5

Molecular formula:

H2O

IUPAC Name:

Oxidane

Test material form:

solid: crystalline

Remarks:

crystalline powder

Details on test material:

Batch No: 11679700
Storage: at room temperature, protected from light

Results and discussion

Melting / freezing point

Key result

Atm. press.:

>= 1 009.4 - <= 1 010.7 hPa

Decomposition:

yes

Decomp. temp.:

ca. 178 °C

Remarks on result:

other: Melting of the test item was not observed below the temperature of decomposition, therefore the test item has no melting temperature.

Any other information on results incl. tables

In the temperature range of 100 – 190 °C, an endothermic effect was observed, which cannot be clearly assigned to the melting of the test item. The endothermic effect was directly followed by an exothermic effect in the temperature range of 190 – 220 °C, with a maximum decomposition energy of -80 J/g. No further thermal effects were observed up to the test end temperature of 500 °C.

No.	Sample weight / mg	Onset of Effect / °C	Range of effect / °C	Weight loss / mg	Atmospheric pressure / hPa
PN13120	17.58	168.64 -	100 - 190 (endo)  190 - 220 (exo)	16.94	1010.7
PN13182	9.16	149.81 -	100 - 190 (endo) 190 - 220 (exo)	3.47	1009.4

To verify the results of the DSC measurement, two additional measurements with the capillary method was performed in the temperature range of 40 - 250 °C with a heating rate of 10 K/min. According to the observations during the test with the capillary method, the first endothermic effect in the temperature range of 100 – 190 °C in the DSC measurements cannot be assigned to the melting of the test item. The test item changed its colour from white to brown starting at a temperature of approx. 178 °C. At a temperature of 184 °C the test item decomposes and expands throughout the capillary. Due to this result, the test item has no melting point before its decomposition starting at a temperature of about 178 °C.

Applicant's summary and conclusion

Conclusions:

The test item Adenosine-5'-monophosphate (AMP) (CAS no. 61-19-8) has no melting point up to its decomposition starting at a temperature of approx. 178 °C at atmospheric pressure (1010 hPa) as determined by differential scanning calorimetry and capillary method according to Regulation EC No. 440/2008 Method A.1. and OECD Test Guideline 102 (1995).

Executive summary:

The test item Adenosine-5'-monophosphate (AMP) (CAS no. 61-19-8) has no melting point up to its decomposition starting at a temperature of approx. 178 °C at atmospheric pressure (1010 hPa) as determined by differential scanning calorimetry and capillary method according to Regulation EC No. 440/2008 Method A.1. and OECD Test Guideline 102 (1995).