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EC number: 401-280-0 | CAS number: 91273-04-0 CM 23-376; REOMET 30
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Remarks:
- Degradation product
- Adequacy of study:
- key study
- Study period:
- 1989-05-17 to 1989-05-19
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Justification for type of information:
- 1. HYPOTHESIS FOR THE ANALOGUE APPROACH
As the substance hydrolyses immediately in contact with water, data from the major constituent of the hydrolysate (bis(2 -ethylhexyl)amine is used to assess this endpoint. This constituent is also the most hazardous constituent to the aquatic environment. For further read across justification see IUCLID chapter 6.1 and CSR chapter 7.1.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
Parent substance EC 401 -280 -0
The acute toxicity of the substance EC 401-280-0 to Daphnia was assessed according to OECD guideline no. 202 (1984). The respective EC50 24 h is 9.3 mg/L, based on nominal concentrations. As the exposure was only 24 hours, this data is only used as supportive information.
Furthermore, as the substance hydrolyses immediately in contact with water, data from the major constituent of the hydrolysate (bis(2 -ethylhexyl)amine is used to assess this endpoint. This constituent is also the most hazardous constituent to the aquatic environment. For further read across justification see IUCLID chapter 6.1 and CSR chapter 7.1.
Hydrolysis products
bis(2 -ethylhexyl)amine
The reported 48-h EC50 of bis(2-ethylhexyl)amine on Daphnia magna was 2.2 mg/L (nominal value) in a test using Tween 80 as a solvent. The EC50 was determined using non-neutralised samples (BASF AG 1/0500/2/89-0500/89, 1989).
Based on the results from a long-term reproduction study with Daphnia magna, the substance is classified as Chronic Category 1. Comparing the available aquatic toxicity data on the substance EC 401 -280 -0 with the data of bis(2 -ethylhexyl)amine shows that aquatic toxicity is in the same range. In most cases the derived effect concentrations of bis(ethyhexyl)amine are lower.Therefore, aquatic toxicity data of the major and the most hazardous component of the hydrolysate (bis(2-ethylhexyl)amine) is used as worst case to assess the acute toxicity to aquatic invertebrates of the substance EC 401-280-0 and its hydrolysate.
3. ANALOGUE APPROACH JUSTIFICATION
The aquatic toxicity data of the major and the most hazardous component of the hydrolysate (bis(2-ethylhexyl)amine) is used as worst case to assess the acute toxicity to aquatic invertebrates of the substance EC 401-280-0 and its hydrolysate. - Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- GLP compliance:
- no
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Tween 80
- Concentration of vehicle in test medium (stock solution): 100 mg/L - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: Inhouse, originally obtained from Institut National de Recherche Chimique Appliquee, France in 1978
- Age at study initiation (range): 2 - 24 h
ACCLIMATION
- Type of food: green algae
- Feeding frequency: daily - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Hardness:
- total: 2.7 ± 0.5 mM
- Test temperature:
- 292 - 294 K
- pH:
- 8.0 ± 0.5
- Nominal and measured concentrations:
- 0.039, 0.078, 0.156, 0.312, 0.624, 1.25, 2.5, 4.99, 10, 20 mg/L as well as a control and a solvent control
- Details on test conditions:
- TEST SYSTEM
- Test vessel: centrifuge tubes
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: artificial freshwater
- Ca/mg ratio: 4:1
- Conductivity: 600 - 700 µSiemens/cm
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0 and 48 h
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod: diffuse light
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
immobility after 0, 3, 6, 24 and 48 hours - Reference substance (positive control):
- no
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 2.2 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC0
- Effect conc.:
- 2.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC100
- Effect conc.:
- 20 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- 0.624 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 48-h EC50 of Daphnia magna exposed to bis(2-ethylhexyl)amine was determined to be 2.2 mg/L.
Reference
Immobile daphnids
Nominal conc.(mg/l) |
Immobile daphnids after |
||||
0 h |
3 h |
6 h |
24 h |
48 h |
|
0 |
0 |
0 |
0 |
0 |
0 |
Vehicle |
0 |
0 |
0 |
0 |
0 |
0.039 |
0 |
0 |
0 |
0 |
0 |
0.078 |
0 |
0 |
0 |
0 |
0 |
0.156 |
0 |
0 |
0 |
0 |
1 |
0.312 |
0 |
0 |
0 |
0 |
2 |
0.624 |
0 |
0 |
0 |
0 |
2 |
1.25 |
0 |
0 |
0 |
0 |
4 |
2.5 |
0 |
0 |
0 |
2 |
11 |
4.99 |
0 |
0 |
0 |
4 |
17 |
10 |
0 |
1 |
1 |
13 |
20 |
20 |
0 |
9 |
11 |
20 |
20 |
pH values
Nominal conc.(mg/l) |
pH values after |
|
0 h |
48 h |
|
0 |
8.05 |
8.08 |
Vehicle |
8.06 |
8.04 |
0.039 |
8.05 |
8.1 |
0.078 |
8.04 |
8.11 |
0.156 |
8.04 |
8.11 |
0.312 |
8.03 |
8.1 |
0.624 |
8.03 |
8.09 |
1.25 |
8.03 |
8.08 |
2.5 |
8.03 |
8.08 |
4.99 |
8.05 |
8.07 |
10 |
8.07 |
8.05 |
20 |
8.09 |
8.02 |
Description of key information
The 48-h EC50 of Daphnia magna exposed to bis(2-ethylhexyl)amine was determined to be 2.2 mg/L. This result is used to assess the acute toxicity of substance EC 401-280-0 and its hydrolysate to aquatic invertebrates.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 2.2 mg/L
Additional information
Parent substance EC 401 -280 -0
The acute toxicity of the substance EC 401-280-0 to Daphnia was assessed according to OECD guideline no. 202 (1984). The respective EC50 24 h is 9.3 mg/L, based on nominal concentrations. As the exposure was only 24 hours, this data is only used as supportive information.
Furthermore, as the substance hydrolyses immediately in contact with water, data from the major constituent of the hydrolysate (bis(2 -ethylhexyl)amine is used to assess this endpoint. This constituent is also the most hazardous constituent to the aquatic environment. For further read across justification see IUCLID chapter 6.1 and CSR chapter 7.1.
Hydrolysis products
bis(2 -ethylhexyl)amine
The reported 48-h EC50 of bis(2-ethylhexyl)amine on Daphnia magna was 2.2 mg/L (nominal value) in a test using Tween 80 as a solvent. The EC50 was determined using non-neutralised samples (BASF AG 1/0500/2/89-0500/89, 1989).
Based on the results from a long-term reproduction study with Daphnia magna, the substance is classified as Chronic Category 1. Comparing the available aquatic toxicity data on the substance EC 401 -280 -0 with the data of bis(2 -ethylhexyl)amine shows that aquatic toxicity is in the same range. In most cases the derived effect concentrations of bis(ethyhexyl)amine are lower.Therefore, aquatic toxicity data of the major and the most hazardous component of the hydrolysate (bis(2-ethylhexyl)amine) is used as worst case to assess the acute toxicity to aquatic invertebrates of the substance EC 401-280-0 and its hydrolysate.
formaldehyde
Acute toxicity of formaldehyde to invertebrates was determined in a study with Daphnia pulex and a LC50 of 5.8 mg/L was derived (Tisler, 1997).
Therefore, the test substance is considered as acutely toxic to aquatic invertebrates.
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