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EC number: 203-818-8 | CAS number: 110-95-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
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- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
The acute oral LD50 of N, N, N', N'-tetramethyltrimethylenediamine is 624 mg/kg in rats and the acute dermal LD50 is 1180 mg/kg in rat. The 4 hour-exposure LC50 is higher than 1000 ppm (5300 mg/m3) in rats.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (limited characterisation of test substance, reduced documentation of test animal welfare)
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 401 (Acute Oral Toxicity)
- Deviations:
- yes
- Remarks:
- (limited documentation on test substance and test animals)
- Principles of method if other than guideline:
- BASF-Test: The study was conducted according to an internal BASF method which in principle is comparable to the OECD Guideline 401.
Test groups consisting of 5 animals/sex was treated by single gavage application with an aqueous solution of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy. - GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Gassner
- Mean weight at study initiation: 189 g (males); 164 g (females) - Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 8 %
MAXIMUM DOSE VOLUME APPLIED: ca. 15 mL/kg bw - Doses:
- 400, 500, 640, 800, 1000, 1250 µL/kg bw (312, 390, 500, 624, 780, 975 mg/kg bw - based on the density d=0.78 g/cm³)
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observation: daily
- Frequency of weighing: days 0, 3, 7 and 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 0.8 mL/kg bw
- Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- 624 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 500 - < 780
- Remarks on result:
- other: based on the density d=0.78 g/cm³
- Mortality:
- 975 mg/kg: 9/10 (2 males and 4 females died within 6 h post application. 3 further males died within 24 h post application.)
780 mg/kg: 7/10 (1 male and 2 females died within 24 h post application. 3 further males and 1 further female died within 7 days post application.)
624 mg/kg: 4/10 (1 female died within 24 h. 2 further females and 1 male died within 7 days.)
500 mg/kg: 1/10 (1 male died within 48 h.)
390 mg/kg: 1/10 (1 female died within 14 days.)
312 mg/kg: 1/10 (1 female died within 48 h.) - Clinical signs:
- other: - 975 and 780 mg/kg: immediately after application: accelerated respiration; 1 h post application: abdominal position, mouth discharge, chewing reflex, in some animals dyspnoea; 3 h post application: red smeared snouts and ruffled fur; 2 days post applica
- Gross pathology:
- Animals that died during observation period: acute passive hyperemia; acute dilatation of the heart; glandular stomach reddened diffusely; isolated ulcerations; diarrhetic contents of the intestines; acinar pattern of the liver
Sacrificed animals: 975 mg/kg: 1x thickened forestomach-epithelium - Interpretation of results:
- Toxicity Category IV
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Executive summary:
The Acute oral toxicity of Tetramethylpropylendiamine (TMPDA) was evaluated in rats according an internal BASF method, which in principle is comparable to the OECD Guideline 401.
A test group consisting of 5 Sprague-Dawley rat/sex was treated by single gavage application with an aqueous solution of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy. The LD50 value was estimated on the basis of the observed mortalities.
At 975 mg/kg, 2 males and 4 females died within 6 h after treatment. 3 further males died within 24 after treatment.
At 780 mg/kg, 1 male and 2 females died within 24 h after treatment. 4 males and 3 females died within 7 days after treatment.
At 624 mg/kg, 1 female died within 24 h. 3 females and 1 male died within 7 days.
At 500 mg/kg, 1 male died within 48 h.
At 390 mg/kg, 1 female died within 14 days. 312 mg/kg: 1 female died within 48 h.
Under these experimental conditions, the oral LD50 of TMPDA is 624 mg/kg in rats.
Reference
Mortality:
Dose: (mg/kg) |
Gender |
1 h |
24 h |
48 h |
day 7 |
day 14 |
975 |
male |
0/5 |
5/5 |
5/5 |
5/5 |
5/5 |
975 |
female |
0/5 |
4/5 |
4/5 |
4/5 |
4/5 |
780 |
male |
0/5 |
1/5 |
3/5 |
4/5 |
4/5 |
780 |
female |
0/5 |
2/5 |
2/5 |
3/5 |
3/5 |
624 |
male |
0/5 |
0/5 |
0/5 |
1/5 |
1/5 |
624 |
female |
0/5 |
1/5 |
1/5 |
3/5 |
3/5 |
500 |
male |
0/5 |
0/5 |
1/5 |
1/5 |
1/5 |
500 |
female |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
390 |
male |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
390 |
female |
0/5 |
0/5 |
0/5 |
0/5 |
1/5 |
312 |
male |
0/5 |
0/5 |
0/5 |
0/5 |
0/5 |
312 |
female |
0/5 |
0/5 |
1/5 |
1/5 |
1/5 |
Weight (g):
Dose (mg/kg) |
Gender |
day 0 |
day 3 |
day 7 |
day 13 |
975 |
male |
168 |
- |
- |
- |
780 |
male |
192 |
171 |
206 |
246 |
624 |
male |
186 |
164 |
184 |
211 |
500 |
male |
194 |
175 |
211 |
231 |
390 |
male |
180 |
174 |
207 |
210 |
312 |
male |
193 |
200 |
229 |
236 |
|
|
|
|
|
|
975 |
female |
163 |
143 |
158 |
159 |
780 |
female |
163 |
149 |
157 |
172 |
624 |
female |
165 |
154 |
149 |
231 |
500 |
female |
164 |
147 |
173 |
173 |
390 |
female |
165 |
146 |
150 |
170 |
312 |
female |
166 |
178 |
168 |
181 |
Harmful if swallowed. There is indication that the test substance causes local irritation to exposed tissue.
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 624 mg/kg bw
Acute toxicity: via inhalation route
Link to relevant study records
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
- Principles of method if other than guideline:
- Six rats are exposed during 4hours to 1000ppm.
- GLP compliance:
- no
- Test type:
- standard acute method
- Limit test:
- yes
- Species:
- rat
- Strain:
- other: Carworth-Wistar
- Sex:
- male
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: no data
- Age at study initiation: no data
- Weight at study initiation: no data
- Fasting period before study: no data
- Housing:no data
- Diet (e.g. ad libitum): no data
- Water (e.g. ad libitum):no data
- Acclimation period:no data
ENVIRONMENTAL CONDITIONS
- Temperature (°C): no data
- Humidity (%): no data
- Air changes (per hr): no data
- Photoperiod (hrs dark / hrs light): no data
IN-LIFE DATES: From: To:no data - Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- air
- Details on inhalation exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: no data
- Exposure chamber volume: no data
- Method of holding animals in test chamber: no data
- Source and rate of air: no data
- Method of conditioning air: no data
- System of generating particulates/aerosols:no data
- Method of particle size determination: no data
- Treatment of exhaust air:no data
- Temperature, humidity, pressure in air chamber: no data
TEST ATMOSPHERE
- Brief description of analytical method used: no
- Samples taken from breathing zone: no - Analytical verification of test atmosphere concentrations:
- no
- Duration of exposure:
- ca. 4 h
- Concentrations:
- 1000 ppm (5.32 mg/L)
- No. of animals per sex per dose:
- 6
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: yes
- Other examinations performed: no data - Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 1 000 ppm
- Exp. duration:
- 4 h
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- > 5.32 mg/L air
- Exp. duration:
- 4 h
- Mortality:
- 2/6
- Clinical signs:
- other: No data
- Body weight:
- No data
- Gross pathology:
- No data
- Interpretation of results:
- Toxicity Category III
- Remarks:
- Migrated information Criteria used for interpretation of results: EU
- Conclusions:
- Under these experimental conditions, the inhalation LC50 of TMPDA is higher than 1000 ppm (5.32 mg/l) in male.
- Executive summary:
The Acute inhalation toxicity of Tetramethylpropylenediamine (TMPDA) was evaluated in one group of 6 rats. The 4-hour exposure at 1000 ppm caused death in 2/6rats.
Under these experimental conditions, the inhalation LC50 of TMPDA is higher than 1000 ppm (5.32 mg/l) in male.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- discriminating conc.
- Value:
- 5 300 mg/m³ air
Acute toxicity: via dermal route
Link to relevant study records
- Endpoint:
- acute toxicity: dermal
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Comparable to guideline study with acceptable restrictions (occlusive treatment)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 402 (Acute Dermal Toxicity)
- Deviations:
- yes
- Remarks:
- (occlusive treatment)
- GLP compliance:
- not specified
- Test type:
- standard acute method
- Limit test:
- no
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Dr. K. Thomae GmbH, Biberach, Germany
- Age at study initiation: about 12 weeks
- Mean weight at study initiation: 212 g (males); 190 g (females)
- Housing: housing of individual animals during the exposure period (24 hours). lf there are no lesions afterward, the animals are kept in groups.
- Diet: Ssniff R; Ssniff Versuchstierdiaeten, Soest, Germany; ad libitum
- Water: fulIy demineralized water ad libitum each workday; tap water ad libitum on public holidays
- Acclimation period: at least 1 week.
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20-26 °C
- Humidity (%): 45-75 %
- Photoperiod (hrs dark / hrs light): 12/12 - Type of coverage:
- occlusive
- Vehicle:
- water
- Details on dermal exposure:
- TEST SITE
- Area of exposure: 50 cm²
REMOVAL OF TEST SUBSTANCE
- Washing (if done): warm water
- Time after start of exposure: 24 h
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 10 ml
- Concentration (if solution): 50%, 10% or 4.64% - Duration of exposure:
- 24 h
- Doses:
- 2500, 1000 or 464 mg/kg bw
- No. of animals per sex per dose:
- 5
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations: Recording of signs and symptoms <15min, 15min, 30min, 1h, 2h, 4h and 5h after test substance administration and then once each workday. Check for moribund and dead animals twice each workday and once daily at weekends and on public holidays.
- Frequency of weighing: day 0, 2, 7 and 13
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight - Sex:
- male/female
- Dose descriptor:
- LD50
- Effect level:
- ca. 1 180 mg/kg bw
- Based on:
- test mat.
- 95% CL:
- > 1 000 - < 2 500
- Remarks on result:
- other: 3/10 animals died within 7 days in the 1000 mg/kg bw dose group; LD50 determined by interpolation
- Mortality:
- - 2500 mg/kg bw: all animals died within 24 h.
- 1000 mg/kg bw: 1 male and 1 female died within 48 h and 1 further female died within 7 days.
- 464 mg/kg bw: no animal died within the observation period. - Clinical signs:
- other: Symptoms: dyspnoea, apathy, excitation, spastic gait, poor general state (see details in table in remarks on results) Local findings: erythema, necrosis, ruptured necrosis, edema, scaling, anaemia, scar formation (see details in table in remarks on result
- Gross pathology:
- Animals that died: application site: severe hyperemia of muscles and subcutis; peritoneum blunt; general passive hyperemia.
Sacrificed animals: several animals with scabbed, extensive necroses at the margin of the application area; striated necroses in isolated animals. - Executive summary:
The acute dermal toxicity of N,N,N',N'-tetramethylpropane-1,3-diamine (TMPDA) was evaluated in rats according to OECD N° 402 guideline. TMPDA was applied to the skin of groups of 5 male and 5 female Wistar rats at doses of 464, 1000 and 2500 mg/kg under an occlusive dressing for 24 hours. The application area was then washed with warm water. Following treatment, rats were observed daily and weighted weekly. A gross necropsy examination was performed at the time of scheduled euthanasia (Day 14). At 2500 mg/kg, all animals died within 24 h. At 1000 mg/kg, 1 male and 1 female died within 48 h and 1 further female died within 7 days. Systemic clinical signs observed were: dyspnea, apathy, excitation, spastic gait, poor general state. Local effects observed were: erythema, necrosis, ruptured necrosis, edema, scaling, anemic, scar formation. Animals found dead showed severe hyperemia of muscles and subcutis, peritoneum blunt and general passive hyperemia at the application site. Sacrificed animals showed scabbed, extensive necroses at the margin of the application area, striated necroses in isolated animals. Under these experimental conditions, the dermal LD50 of TMPDA is 1180 mg/kg in male and female Wistar rats.
Reference
Mortality:
Dose (mg/kg) |
Gender |
Conc. (%) |
1 h |
24 h |
48 h |
day 7 |
day 14 |
2500 |
male |
50 |
0 |
5 |
5 |
5 |
5 |
2500 |
female |
50 |
0 |
5 |
5 |
5 |
5 |
1000 |
male |
10 |
0 |
0 |
1 |
1 |
1 |
1000 |
female |
10 |
0 |
0 |
1 |
2 |
2 |
464 |
male |
4.64 |
0 |
0 |
0 |
0 |
0 |
464 |
female |
4.64 |
0 |
0 |
0 |
0 |
0 |
Weight: (g)
Dose (mg/kg) |
Gender |
day 0 |
day 2 |
day 7 |
day 13 |
2500 |
male |
207 |
- |
- |
- |
1000 |
male |
215 |
197 |
223 |
271 |
464 |
male |
214 |
203 |
241 |
277 |
2500 |
female |
186 |
- |
- |
- |
1000 |
female |
192 |
181 |
191 |
217 |
464 |
female |
194 |
186 |
204 |
216 |
Signs and symptoms:
Symptoms: |
2500 mg/kg bw |
1000 mg/kg bw |
464 mg/kg bw |
|||
|
male |
female |
male |
female |
male |
female |
Dyspnoea |
15 min - 4 h |
15 min - 4 h |
30 min - 6 d |
30 min - 6 d |
30 min - 5 d |
30 min - 5 d |
Apathy |
15 min - 4 h |
15 min - 4 h |
30 min - 6 d |
30 min - 6 d |
30 min - 5 d |
30 min - 5 d |
Excitation |
< 15 min |
< 15 min |
- 15 min |
- 15 min |
- 15 min |
- 15 min |
Spastic gait |
15 min - 4 h |
15 min - 4 h |
2 d |
2 d- 5 d |
2 d |
2 d |
Aggressiveness |
- |
- |
- |
2 d |
- |
- |
Poor general state |
< 15 min - 4 h |
< 15 min - 4 h |
30 min - 6 d |
15 min - 6 d |
30 min - 5 d |
30 min - 5 d |
min: minutes; h: hours; d: day
Local findings:
Dose (mg/kg) |
2500 |
1000 |
464 |
Erythema |
- |
7 d -13 d |
7 d - 13 d |
Necrosis |
- |
1 d - 13 d |
1 d - 13 d |
Ruptured necrosis |
- |
13 d |
- |
Edema |
- |
1 d - 13 d |
1 d - 7 d |
Scaling |
- |
7 d - 13 d |
7 d |
Anaemia |
- |
1 d |
1 d |
Scar formation |
- |
- |
13 d |
h: hours; d: day
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 1 180 mg/kg bw
Additional information
Acute oral
The Acute oral toxicity of N, N, N', N'-tetramethyltrimethylenediamine (TMPDA) was evaluated in rats according an internal BASF method, which in principle is comparable to the OECD Guideline 401 (BASF, 1974). A test group consisting of 5 Sprague-Dawley rat/sex was treated by single gavage application with an aqueous solution of the test substance. The animals were observed for mortality and for clinical symptoms of toxicity. At the end of the observation period of 14 days, the surviving animals were sacrificed for the purpose of necropsy; animals that died during the observations period also were subjected to necropsy. The LD50 value was estimated on the basis of the observed mortalities.
At 975 mg/kg, 2 males and 4 females died within 6 h after treatment. 3 further males died within 24 after treatment.
At 780 mg/kg, 1 male and 2 females died within 24 h after treatment. 4 males and 3 females died within 7 days after treatment.
At 624 mg/kg, 1 female died within 24 h. 3 females and 1 male died within 7 days.
At 500 mg/kg, 1 male died within 48 h.
At 390 mg/kg, 1 female died within 14 days. 312 mg/kg: 1 female died within 48 h.
Under these experimental conditions, the oral LD50 of TMPDA is 624 mg/kg in rats.
According to Smyth (1969), the oral LD50 of TMPDA is 0.41 ml/kg (0.31 -0.53) or 320 mg/kg (242-414mg/kg) in male Carworth-Wistar rats.
Acute inhalation
The Acute inhalation toxicity of N, N, N', N'-tetramethyltrimethylenediamine (TMPDA) was evaluated in one group of 6 rats (Smyth 1969). The 4 hour-exposure at 1000 ppm caused death in 2/6 rats and allowed to determine an inhalation LC50 higher than 1000 ppm (5300 mg/m3).
The toxicity of an atmosphere saturated with vapour of TMPDA was evaluated at the temperature 20 °C (BASF, 1974). Groups of 6 male and female rats were exposed to the vapour, generated by bubbling 200 L/h air through a substance column of about 5 cm above a fritted glass disc in a glass cylinder for 10 min or 30 min. The documentation of mortality and clinical signs was performed over a period of 8 days. One male died 1 hour after a 30-min exposure to 39 mg/L. During exposure at this concentration, escape attempts, gasping, clear partially bloody nose discharge were observed, immediately after exposure: animals showed imbalance, accelerated respiration and apathy, after 24 h, slight dyspnoea and apathy, bloody nose crusts were noted. All symptoms were reversible within 4 days. No animals died within the observation period after a 10 min exposure to 40.3 mg/L. During exposure at this concentration, escape attempts, clear partially bloody nose and eye discharge, and gasping were observed. All symptoms were reversible within 1 day. All animals gained weight. General venous passive hyperemia, slight pulmonary edema, and acute dilatation of the heart bilaterally were observed in animal that died. No abnormalities were observed in the sacrificed animals.
Acute dermal
The acute dermal toxicity of N, N, N', N'-tetramethyltrimethylenediamine (TMPDA) was evaluated in rats according to OECD N° 402 guideline (BASF, 1982). TMPDA was applied to the skin of groups of 5 male and 5 female Wistar rats at doses of 464, 1000 and 2500 mg/kg under an occlusive dressing for 24 hours. The application area was then washed with warm water. Following treatment, rats were observed daily and weighted weekly. A gross necropsy examination was performed at the time of scheduled euthanasia (Day 14). At 2500 mg/kg, all animals died within 24 h. At 1000 mg/kg, 1 male and 1 female died within 48 h and 1 further female died within 7 days. Systemic clinical signs observed were: dyspnea, apathy, excitation, spastic gait, poor general state. Local effects observed were: erythema, necrosis, ruptured necrosis, edema, scaling, anemic, scar formation. Animals found dead showed severe hyperemia of muscles and subcutis, peritoneum blunt and general passive hyperemia at the application site. Sacrificed animals showed scabbed, extensive necroses at the margin of the application area, striated necroses in isolated animals. Under these experimental conditions, the dermal LD50 of TMPDA is 1180 mg/kg in male and female Wistar rats.
The acute dermal toxicity of N, N, N', N'-tetramethyltrimethylenediamine (TMPDA) was evaluated in 4 male rabbits. Animals were immobilized during the 24-hour contact and then caged during the 14 day-observation period (Smyth et al., 1969). Under these experimental conditions, the dermal LD50 of TMPDA is 234 mg/kg (164 -328 mg/kg) in rabbits.
Justification for selection of acute toxicity – oral endpoint
Key study
Justification for selection of acute toxicity – inhalation endpoint
Key study
Justification for selection of acute toxicity – dermal endpoint
Key study
Justification for classification or non-classification
Acute oral toxicity:
The oral LD50of N, N, N', N'-tetramethyltrimethylenediamine was 624 mg/kg in rats. In accordance with Regulation (EC) No 1272/2008, TMPDA shall be classified as Acute Tox. 4 (Hazard statement: H302; Harmful if swallowed) and in accordance with Annex VI of Commission Directive 2001/59/EC, TMPDA shall be classified as being harmful if swallowed (R22).
Acute inhalation toxicity:
The LC50of N, N, N', N'-tetramethyltrimethylenediamine was higher than 5.3 mg/L in rats (but probably lower than 10 mg/L). On the basis of this study and in accordance with Regulation (EC) No 1272/2008 TMPDA shall be classified as Acute Tox. 3 (Hazard statement: H331; Toxic if inhaled) and in accordance with Annex VI of Commission Directive 2001/59/EC, N, N, N', N'-tetramethyltrimethylenediamine shall be classified as being harmful by inhalation (R20).
Acute dermal toxicity:
The percutaneous LD50 of N, N, N', N'-tetramethyltrimethylenediamine was 1180 mg/kg in rats. On the basis of this study and in accordance with Regulation (EC) No 1272/2008 TMPDA shall be classified as Acute Tox. 4 (Hazard statement: H312; Harmful by skin contact) and in accordance with Annex VI of Commission Directive 2001/59/EC, N, N, N', N'-tetramethyltrimethylenediamine shall be classified as being harmful by skin contact (R21).
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