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EC number: 814-155-4 | CAS number: 13188-82-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 21.12.2018 to 17.07.2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 310 (Ready Biodegradability - CO2 in Sealed Vessels (Headspace Test)
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic (adaptation not specified)
- Details on inoculum:
- SOURCE
Activated sludge from the aeration tank from the waste water sewage plant Mannheim, Germany
CULTIVATION
A suitable aliquot of the suspension was sieved by a finely woven mesh with a mesh size of about 1 mm. Subsequently, this sludge suspension was aerated for about 4 days at 20 ± 2° C with carbon dioxide free air to reduce the inorganic carbon content in the blank controls.
PREPARATION FOR EXPOSURE
At the day of exposure, the aeration was stopped, the sludge suspension was washed for one time using drinking water and was left to settle. The supernatant was discarded, and the residue of the sludge suspension was filled up again with drinking water and the sludge of the suspension was adjusted to a final concentration 800 mg/L of dry weight. Aliquots of 0.5 mL from this suspension were added to the test vessels to obtain a sludge concentration of 4 mg/L dry substance in the test assays.
- Duration of test (contact time):
- 28 d
- Initial conc.:
- 34 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- TOC
- Parameter followed for biodegradation estimation:
- inorg. C analysis
- Details on study design:
- TEST CONDITIONS
- Composition of medium: see table 1
- Test temperature: 20 +- 2°C
- pH: 7.4
- pH adjusted: adjusted to 7.4 with 1 molar sulphuric acid
- Other: samples were shaken on an orbital shaker at a speed of about 150 rpm
TEST SYSTEM
- Method used to create aerobic conditions: bottles were sealed with butyl rubber septa and aluminum caps
- Measuring equipment:
- Details of trap for CO2 and volatile organics if used:
- Other:
SAMPLING
On each sampling days (Day 3, 5, 7, 12, 14, 19, 24, 26) three bottles of each test assay were sacrificed. On the last day (Day 28) of exposure, five bottles were sacrificed for the test substance assay. During sampling, one mL of 7 molar NaOH solution was injected with a syringe through the septum of each bottle. The bottles were shaken for about one hour afterwards. Carbon dioxide present in the headspace that was evolved during biological processes is dissolved in the alkaline solution during this process. Afterwards, the bottles were opened and the total inorganic carbon (TIC) content trapped in the sodium hydroxide solution during the alkalization of the test assays was determined with a carbon analyzer
- Sample storage before analysis: the samples were analyzed on the same day
TEST/ CONTROL SYSTEM
29 blank control assays
31 test substance assays
29 inhibition control test assays
17 reference substance assays
5 assays for abiotic control: (mercuric chloride was added to inhibit the possible microbial growth)
10 test assays for additional pH and oxygen analysis
CALCULATION/ STATISTICAL METHODS:
Single values of these test assays were used for mean value calculation of the biodegradation degree with a limit of confidence limit of 95%. The measured TIC-values were compared with the added amount of TOC and expressed as biodegradation degree and plotted as biodegradation curve. - Reference substance:
- aniline
- Preliminary study:
- Determination of total organic carbon by combustion
- Parameter:
- % degradation (inorg. C analysis)
- Value:
- 33
- St. dev.:
- 5
- Sampling time:
- 28 d
- Details on results:
- Duration of the adaptation phase: approx.8 days
Degree of biodegradation of the test substance at the end of
the ten-days window, mean value: approx. 22% TIC/TOC
Degree of biodegradation of the test substance at the end of
exposure (mean value ±SD of five test assays, limit of
confidence 95%): 33 ±5% TIC/TOC
Degree of elimination of the test substance at physical
chemical control at the end of exposure, mean value: <10% TIC/TOC - Results with reference substance:
- - Reference substance: aniline
- Degree of biodegradation of the reference substance after 14 days, mean value: 83% TIC/TOC
- Degree of biodegradation in the inhibition control after 14 days, mean value: 50% TIC/TOC
- Test temperature: 20 +- 2 °C - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- The test item was not readily biodegradable under the conditions of this test over the 28-day exposure to activated sludge according to OECD guideline 310. The required pass level for ready biodegradability i.e., at least 60% of the theoretical TIC/TOC production in a 10- day window exceeding 10% degradation within the 28-day period of the test was not reached.
- Executive summary:
The biodegradability of the test item was assessed according to the OECD guideline 310 (headspace test). The test item (34 mg/L) was exposed to 4 mg/l (dry weight) activated sewage sludge for 28 d under aerobe conditions in test bottles closed with butyl rubber septa. The inoculum was collected from the aeration tank of a municipal sewage treatment plant. As reference substance aniline was used. After day 3, 5, 7, 12, 14, 19, 24 and 26 three test bottle were analyzed, and five bottles were analyzed on day 28. After 28 days 33 ± 5 % were degraded the 10 day window criteria was not met and the substance cannot be considered readily biodegradable according to OECD criteria.
Reference
Description of key information
The test item can not be considered readily biodegradable according to OECD criteria.
Key value for chemical safety assessment
- Biodegradation in water:
- inherently biodegradable, not fulfilling specific criteria
- Type of water:
- freshwater
Additional information
The biodegradability of the test item was assessed according to the OECD guideline 310 (headspace test). The test item (34 mg/L) was exposed to 4 mg/l (dry weight) activated sewage sludge for 28 d under aerobe conditions in test bottles closed with butyl rubber septa. The inoculum was collected from the aeration tank of a municipal sewage treatment plant. As reference substance aniline was used. After day 3, 5, 7, 12, 14, 19, 24 and 26 three test bottle were analyzed, and five bottles were analyzed on day 28. After 28 days 33 ± 5 % were degraded the 10 day window criteria was not met and the substance cannot be considered readily biodegradable according to OECD criteria.
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