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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 2005-03-01 to 2005-03-08
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.6800 (Modified Activated Sludge, Respiration Inhibition Test for Sparingly Soluble Chemicals)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- - Substance type: Organic
- Physical state: off-white powder
- Composition of test material, percentage of components: component 1 ca 50%, component 2 ca 30%, component 3 ca 20%
- Lot/batch No.: 050210A
- Expiration date of the lot/batch: February 2007
- Storage condition of test material: room temperature in the dark - Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
See "any other information on materials and methods" - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- A sample of activated sludge was obtained the day before the start of the test from Worlingworth Sewage Treatment Works, which treats predominantly domestic waste. In the laboratory, the sample was maintained under aerobic conditions until required.
The concentration of suspended solids in a homogenised sample was determined on the day of collection and immediately before the start of the test.
On the day of collection, aliquots (25 mL) of the activated sludge were filtered through dried and preweighed Whatman GF/C filter papers which were then dried again at approximately 105°C for one hour, allowed to cool in a desiccator and reweighed. The mixed liquor suspended solids (MLSS) content of the activated sludge was then calculated. Synthetic sewage (50 mL/L) was added to the sample of activated sludge and the mixture aerated overnight.
On the day of the test, the MLSS content of the sludge was detennined and adjusted to 4 g/L by the addition of dechlorinated tap water. The pH of the sludge was also measured. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Post exposure observation period:
- None
- Hardness:
- Not reported
- Test temperature:
- See table 2 attached background information
- pH:
- 3,5-DCP stock solution 7.6
Activated sludge 7.3
See table 2 attached background information - Dissolved oxygen:
- See table 1 attached background material
- Nominal and measured concentrations:
- Nominal: 1, 10, 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): open / test beakers covered with loosely fitting aluminium foil
- Material, size, headspace, fill volume: one-litre size, fill volume approximately 600mL
- Aeration: yes for 3 hours
- No. of vessels per concentration (replicates): 1 at 1 and 10 mg/L; 3 at 100mg/L
- No. of vessels per control (replicates): 1 each for positive control, 2 for antifoam-treated control, 1 control without antifoaming agent
- Biomass loading rate: 4 g/L
TEST MEDIUM / WATER PARAMETERS
Synthetic sewage feed for activated sludge was prepared by dissolving the following in one litre of ultrapure water (nominal resistivity, >= 0: 18 MegOhm.cm):
peptone 16.0 g
meat extract 11.0 g
urea 3.0 g
sodium chloride 0.7 g
calcium chloride dihydrate 0.4 g
magnesium sulphate heptahydrate 0.2 g
di-potassium hydrogen phosphate 2.8 g
OTHER TEST CONDITIONS
- Adjustment of pH: none reported
- Photoperiod: none
- Light intensity: not applicable
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :
The rate of oxygen consumption was measured, over a period of approximately ten minutes or until the dissolved oxygen concentration fell below 2 mgO2/L, using a Yellow Springs Instruments (YSI) dissolved oxygen meter, with temperature probe and self-stirring bottle probe, connected to a chart recorder. The pH and temperature of the samples were measured at the start and end of the test.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The test item was considered to have had no biologically significant inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. Sludge respiration was decreased by 1% of the mean control rate in one mixtures containing test item at 100 mg/L. The EC20, EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/L, the highest level tested.
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 5.5 mg/L
- Other:
Specific respiration rates were progressively reduced in the presence of increasing concentrations of 3,5-DCP. The three-hour 50% effect concentration (EC50) for 3,5-DCP was to be 5.5 mg/L (95% confidence limits 3.6 - 8.2 mg/L). - Validity criteria fulfilled:
- yes
- Conclusions:
- The test item was considered to have had no inhibitory effect on the respiration rate of activated sludge at any of the concentrations employed in the test. The EC20 , EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/L, the highest level tested.
- Executive summary:
The effect of test item on the respiration rate of activated sludge was assessed by the methods detailed in EC Directive 88/302 and OECD Test Guideline 209. Samples of activated sludge (suspended solids 1.6 g/litre), fed with synthetic sewage, were exposed to the test substance at nominal concentrations of 1, 10 and 100 mg/litre for three hours. Single mixtures were prepared at 1 and 10 mg/litre and the highest level was prepared in triplicate. Their rates of oxygen consumption were determined and compared with those of controls, containing activated sludge and synthetic sewage alone, which were established at the beginning and end of the culture series. The reference inhibitor 3,5-dichlorophenol (3,5-DCP) was employed at 3, 10 and 32 mg/litre, as a positive control. An antifoaming agent (silicone based, oil in water emulsion) was added to selected sludge mixtures to assist dispersal of the test substance and suppress foam development which may have caused the test substance to deposit above the surface of the liquors. The specific respiration rate of the antifoam-treated control cultures, established at the beginning and end of the test series (25.4 mgO2/g/h), and the control without antifoaming agent (25.4 mgO2/g/h) confirmed that the test precision was acceptable and that the antifoam had had no effect on the activity of the sludge inoculum. The three-hour 50% effect concentration (ECso) for 3,5-DCP was calculated to be 5.5 mg/L (95% confidence limits 3.6 - 8.2 mg/L). These results show that the test was valid and that the sample of activated sludge employed was sensitive to inhibition. The test item was considered to have had no biologically significant inhibitory effect on the respiration rate of activated sludge in the test. The specific respiration rate was decreased by 1% of the mean control value in one mixture containing the test substance at 100 mg/litre. The EC20, EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/L, the highest level tested.
Reference
Description of key information
The effect of test item on the respiration rate of activated sludge was assessed by the methods detailed in EC Directive 88/302 and OECD Test Guideline 209. The test item was considered to have had no biologically significant inhibitory effect on the respiration rate of activated sludge in the test. The specific respiration rate was decreased by 1% of the mean control value in one mixture containing the test substance at 100 mg/litre. The EC20, EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/L, the highest level tested.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 100 mg/L
Additional information
The EC20, EC50 and EC80 of the test substance could not, therefore, be calculated but these must be greater than 100 mg/L, the highest level tested.
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