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Diss Factsheets
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EC number: 419-800-1 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- yes
- Remarks:
- No analytical monitoring of test concentrations conducted
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- yes
- Remarks:
- No analytical monitoring of test concentrations conducted
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- A 100 mg/L stock solution of the substance in dilution water was prepared by direct additions of the test substance to dilution water. This solution was used to prepare serial dilutions containing 0.1, 1 and 10 mg/L. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna
- Strain/clone: Straus
- Justification for species other than prescribed by test guideline: NA
- Age at study initiation (mean and range, SD): Less than 24 hours
- Source: Culture derived from Shell Research, Sittingbourne UK
- Age of parental stock (mean and range, SD): Third brood onwards from adults aged between 14 and 42 days.
- Feeding during test: No
ACCLIMATION
- Acclimation period: Cultures are maintained for 42 days
- Acclimation conditions (same as test or not): Same as test
- Type and amount of food: The cultures were fed daily with a cocentrated suspension of Chlorella vulgaris algae at approximately 5x10^5 cells/mL. This is obtained from 5L cultures grown under semi-axenic conditions.
- Feeding frequency: Daily
- Health during acclimation (any mortality observed): Juveniles for testing are not taken from cultures which contain adults bearing ephippia.
METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES, INCLUDING CULTURING CONDITIONS: Twenty four hours before a test is setup, juveniles present in the cultures are removed and discarded. Juveniles for use in the test are then removed from the culture and transferred to fresh culture medium. The juveniles are then left for at least one hour before selecting actively swimming individuals for use. All juveniles used in the test are less than 24 hours old. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Not applicable
- Post exposure observation period:
- Not applicable
- Hardness:
- 0 hours: 207 mgCaCO3/L
48 hours: 210 mgCaCO3/L - Test temperature:
- 0 hours: 19.7 to 19.9°C
48 hours: 19.7°C - pH:
- 0 hours: 7.5 to 7.9
48 hours: 8.2 to 8.3 - Dissolved oxygen:
- 0 hours: 90 to 91 % air saturation
48 hours: 96 to 99 % air saturation - Salinity:
- NA
- Conductivity:
- Not specified
- Nominal and measured concentrations:
- Nominal: 0.1, 1, 10 and 100 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Crystalline dishes
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 150 mL containing 100 mL of test medium
- Volume of solution: 100 mL
- Aeration: No
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): Four replicate dishes at 100 mg/L and and two replicate dishes at the remaining concentrations.
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): NA
- Biomass loading rate: 5 Daphnids per 100 mL.
TEST MEDIUM / WATER PARAMETERS
- The water used for culturing and testing the D.magna is reconstituted freshwater prepared by mixing treated mains water and a proprietary mineral water in the rato 1:1. The treated mains dilution water is the laboratory mains supply. The water is pumped to the laboratory via a Purite AC9 activated carbon filter and a Purite ROPF 20 particulate filter.
OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: Not specified
- Light intensity: Not specified
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Immobility at 24 and 48 hours. D. Magna were considered to be immobile if, when the contents of the dish were briefly swirled, they did not swim during a 15 second period of observation.
VEHICLE CONTROL PERFORMED: No
RANGE-FINDING STUDY
- Test concentrations: NA
- Results used to determine the conditions for the definitive study: Combined range-finder and limit test conducted. - Reference substance (positive control):
- no
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities:
- Observations on body length and weight: Not specified
- Other biological observations: Not specified
- Mortality of control: None
- Other adverse effects control: None noted
- Immobilisation of control: None
- Abnormal responses: None noted
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: All stock solutions appeared colourless and it was assumed that all the test substance was in solution.
- Effect concentrations exceeding solubility of substance in test medium: No - Results with reference substance (positive control):
- Reference substance not included
- Reported statistics and error estimates:
- No statistical analysis was performed as no immobility at test concentrations were observed.
- Validity criteria fulfilled:
- yes
- Conclusions:
- At the highest tested concentration and usual limit concentration for these tests (100 mg/L), no immobility of the D.magna was observed. Therefore, the 48 hour EC50 values is > 100 mg/L (the highest concentration tested).
Reference
Immobilisation of D.magna exposed to the substance.
Substance (mg/L) |
Number of D.magna |
Number immobilised |
|
24 h |
48 h |
||
Control |
20 |
0 |
0 |
0.1 |
10 |
0 |
0 |
1 |
10 |
0 |
0 |
10 |
10 |
0 |
0 |
100 |
20 |
0 |
0 |
Description of key information
An EC/OECD method study is available assessing the potential toxicity of the substance to Daphnia magna. No effects were observed at the limit concentration in this study which provided a 48 hour EC50 value of > 100 mg/L.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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