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EC number: 608-630-3 | CAS number: 31528-46-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 16 January 2018 - 15 February 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- 17 July 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ISO International Standard 10634. "Water Quality - Guidance for the preparation and treatment of poorly water-soluble organic compounds for the subsequent evaluation of their biodegradability in an aqueous medium"
- Version / remarks:
- 1995
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- (1R,3aS,3bR,9aR,9bS,11aS)-1-ethynyl-1-hydroxy-11a-methyl-1H,2H,3H,3aH,3bH,7H,8H,9H,9aH,9bH,10H,11H,11aH-cyclopenta[a]phenanthren-7-one
- EC Number:
- 608-630-3
- Cas Number:
- 31528-46-8
- Molecular formula:
- C20H24O2
- IUPAC Name:
- (1R,3aS,3bR,9aR,9bS,11aS)-1-ethynyl-1-hydroxy-11a-methyl-1H,2H,3H,3aH,3bH,7H,8H,9H,9aH,9bH,10H,11H,11aH-cyclopenta[a]phenanthren-7-one
- Test material form:
- solid: particulate/powder
- Details on test material:
- Appearance: Off-white powder
Test item storage: At room temperature
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge: municipal sewage treatment plant: 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.
- Storage conditions: freshly obtained sludge was kept under continuous aeration until further treatment.
- Preparation of inoculum for exposure: before use, the sludge was coarsely sieved (1 mm) and washed with mineral medium.
- Pre-treatment: the day before the start of the test (day -1) mineral components, Milli-RO water (ca. 80% of final volume) and inoculum (1% of final volume) were added to each bottle. This mixture was aerated with synthetic air overnight to purge the system of CO2.
- Initial concentration of sludge (suspended solids): 3.0 g/L - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 12 mg/L
- Based on:
- TOC
- Initial conc.:
- 15 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: mineral medium according to OECD 301B
- Test temperature: 22 - 23°C
- pH:
at the start: 7.6
on day 14 (in the positive control and the toxicity control): 7.7
on day 28 (in the blank controls and the test solutions): 7.5-7.6
- pH adjusted: yes, at the start of the test the pH was adjusted to 7.6 using 1 M HCl
- Suspended solids concentration: the initial sludge (3.0 g/L) was used as inoculum at the amount of 5 mL/L of mineral medium, leading to a SS concentration of 15 mg/L.
- Continuous darkness: yes
- Other: The test solutions were continuously stirred during the test, to ensure optimal contact between the test item and the test organisms. Furthermore, the test medium was daily swirled around, since the test item tended to float on the water surface.
TEST SYSTEM
- Culturing apparatus: 2 litre brown coloured glass bottles
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: synthetic air (CO2 < 1 ppm) sparged through scrubbing solutions at a rate of approximately 1-2 bubbles per second (ca. 30-100 mL/min).
- Measuring method: produced CO2 reacted with barium hydroxide Ba(OH)2 in the gas scrubbing bottle and precipitated out as barium carbonate. The amount of CO2 produced was determined by titrating the remaining Ba(OH)2 with 0.05 M standardized HCl.
SAMPLING
- Sampling frequency: Titrations were made every second or third day during the first 10 days, and thereafter at least every fifth day until day 28, for the inoculum blank and test suspension. Titrations for the positive and toxicity control were made over a period of at least 14 days.
- Sampling method: Each time the CO2-absorber nearest to the test bottle was removed for titration; each of the remaining two absorbers was moved one position in the direction of the test bottle. A new CO2-absorber was placed at the far end of the series. Phenolphthalein (1% solution in ethanol, Merck) was used as pH-indicator.
On the penultimate day, the pH of respective test suspensions was measured and 1 mL of concentrated HCl (37%, Merck) was added to the bottles of the inoculum blank and test suspension. The bottles were aerated overnight to drive off CO2 present in the test suspension.
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, containing only inoculum (2 bottles)
- Abiotic sterile control: no
- Toxicity control: yes, containing test item, reference item and inoculum (1 bottle).
- Other: positive control: containing reference item and inoculum (1 bottle).
STATISTICAL METHODS: no statistics were used
TEST CONCENTRATIONS:
Test substance (added as weighed amounts to the bottles): bottle A: 15.1 mg/L test substance; bottle B: 14.95 mg/L test substance
Reference control: 40 mg/L reference substance
Toxicity control: 14.95 mg/L test substance and 40 mg/L reference substance
Reference substance
- Reference substance:
- acetic acid, sodium salt
- Remarks:
- Purity: 99.5%
Results and discussion
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 9
- Sampling time:
- 28 d
- Remarks on result:
- other: Bottle A
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 2
- Sampling time:
- 28 d
- Remarks on result:
- other: Bottle B
- Details on results:
- - The theoretical CO2 production of the test substance was calculated to be 2.97 mg CO2/mg and that of the reference substance was calculated to be 1.07 mg CO2/mg.
- The test item degraded for 9% and 2% in duplicate bottles tested
- The criterium of at least 60% biodegradation within a 10-day window was not met. Therefore, the substance can not be considered readily biodegradable.
- In the toxicity control, more than 25% biodegradation occurred within 14 days (40%, based on ThCO2). Therefore, the test item was assumed not to inhibit microbial activity.
- Functioning of the test system was checked by testing the reference item sodium acetate (90% biodegradation in 14 days)
BOD5 / COD results
- Results with reference substance:
- Sodium acetate showed a biodegradation of 90% after 14 days.
Any other information on results incl. tables
Table 1 CO2 Production and Percentage Biodegradation of the Reference Item
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation* (%) |
||
Blank (mean) |
Positive control |
||||||
2 |
47.33 |
27.85 |
19.48 |
21.4 |
21.4 |
25 |
|
5 |
44.65 |
20.48 |
24.17 |
26.6 |
48.0 |
56 |
|
8 |
44.70 |
31.58 |
13.12 |
14.4 |
62.4 |
73 |
|
12 |
45.60 |
39.16 |
6.44 |
7.1 |
69.5 |
81 |
|
15#) |
46.34 |
39.58 |
6.76 |
7.4 |
77.0 |
90 |
|
*): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of sodium acetate: 85.6 mg CO2/2L. |
|
Table 2 CO2 Production and Percentage Biodegradation of the Test Item (Bottle A)
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation* (%) |
||
Blank (mean) |
Bottle A |
||||||
2 |
47.33 |
46.83 |
0.50 |
0.5 |
0.5 |
1 |
|
5 |
44.65 |
43.28 |
1.36 |
1.5 |
2.0 |
2 |
|
8 |
44.70 |
43.07 |
1.63 |
1.8 |
3.8 |
4 |
|
12 |
45.60 |
44.35 |
1.25 |
1.4 |
5.2 |
6 |
|
15 |
46.34 |
45.17 |
1.17 |
1.3 |
6.5 |
7 |
|
19 |
46.44 |
45.92 |
0.52 |
0.6 |
7.1 |
8 |
|
23 |
46.99 |
46.48 |
0.51 |
0.6 |
7.6 |
8 |
|
29# |
44.83 |
44.85 |
0.00 |
0.0 |
7.6 |
8 |
|
29# |
47.42 |
46.77 |
0.65 |
0.7 |
8.3 |
9 |
|
29# |
49.23 |
49.33 |
0.00 |
0.0 |
8.3 |
9 |
|
*): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test item: 89.7 mg CO2/2L. #): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl. |
|
Table 3 CO2 Production and Percentage Biodegradation of the Test Item (Bottle B)
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation* (%) |
||
Blank (mean) |
Bottle B |
||||||
2 |
47.33 |
47.30 |
0.03 |
0.0 |
0.0 |
0 |
|
5 |
44.65 |
43.49 |
1.15 |
1.3 |
1.3 |
1 |
|
8 |
44.70 |
44.53 |
0.17 |
0.2 |
1.5 |
2 |
|
12 |
45.60 |
45.88 |
0.00 |
0.0 |
1.5 |
2 |
|
15 |
46.34 |
47.16 |
0.00 |
0.0 |
1.5 |
2 |
|
19 |
46.44 |
46.77 |
0.00 |
0.0 |
1.5 |
2 |
|
23 |
46.99 |
47.36 |
0.00 |
0.0 |
1.5 |
2 |
|
29# |
44.83 |
45.97 |
0.00 |
0.0 |
1.5 |
2 |
|
29# |
47.42 |
48.14 |
0.00 |
0.0 |
1.5 |
2 |
|
29# |
49.23 |
49.76 |
0.00 |
0.0 |
1.5 |
2 |
|
*): Calculated as the ratio between CO2produced (cumulative) and the ThCO2of the test item: 88.8 mg CO2/2L. #): CO2measured on day 29 is actually part of CO2production of day 28, since microbial activity was ended on day 28 by addition of HCl. |
|
Table 4 CO2 Production and Percentage Biodegradation of the Toxicity Control
Day |
HCl (0.05 N) titrated (mL) |
Produced CO2 (mL HCl) |
Produced CO2 (mg) |
Cumulative CO2 (mg) |
Biodegradation* (%) |
|
Blank (mean) |
Toxicity control |
|||||
2 |
47.33 |
30.06 |
17.27 |
19.0 |
19.0 |
11 |
5 |
44.65 |
23.59 |
21.06 |
23.2 |
42.2 |
24 |
8 |
44.70 |
31.58 |
13.12 |
14.4 |
56.6 |
32 |
12 |
45.60 |
39.05 |
6.55 |
7.2 |
63.8 |
37 |
15# |
46.34 |
40.18 |
6.16 |
6.8 |
70.6 |
40 |
*): Calculated as the ratio between CO2produced (cumulative) and the sum of the ThCO2of the test item and positive control: 174.4 mg CO2/2L (ThCO2test item: 88.8 mg CO2/2L + ThCO2sodium acetate: 85.6 mg CO2/2L). #): CO2measured on day 15 is actually part of CO2production of day 14, since microbial activity was ended on day 14 by addition of HCl. |
Table 5 Comparison of Biodegradation of the Test Item in Bottles A and B
Day |
Biodegradation (%) |
|||
Bottle A |
Bottle B |
Mean A and B |
∆ A-B* |
|
2 |
1 |
0 |
1 |
1 |
5 |
2 |
1 |
2 |
1 |
8 |
4 |
2 |
3 |
2 |
12 |
6 |
2 |
4 |
4 |
15 |
7 |
2 |
5 |
5 |
19 |
8 |
2 |
5 |
6 |
23 |
8 |
2 |
5 |
6 |
29# |
8 |
2 |
5 |
6 |
29# |
9 |
2 |
6 |
7 |
29# |
9 |
2 |
6 |
7 |
*): Absolute difference in biodegradation between bottles A and B |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- see 'overall remarks'
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- A ready biodegradation test (Modified Sturm test), performed according to OECD 301B and GLP principles, showed that D6-Noreth was biodegraded for 9% and 2% in the duplicate bottles in 28 days. Since the pass level of 60% biodegradation within a 10-day window was not met, D6-Noreth is determined to be not readily biodegradable under the conditions of this test.
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