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EC number: 200-606-7 | CAS number: 65-30-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Description of key information
Acute toxicity: Oral
Acute oral toxicity study of the test chemical was conducted in rats at the dose concentration of 50 mg/kg bw. 50% mortality was observed. Hence, LD50 value was considered to be 50 mg/kg bw, when rats were treated with the test chemical via oral route.
Key value for chemical safety assessment
Acute toxicity: via oral route
Link to relevant study records
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Justification for type of information:
- Data is from handbook.
- Qualifier:
- according to guideline
- Guideline:
- other: As mentioned below
- Principles of method if other than guideline:
- Acute oral toxicity of the test chemical in rat.
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
- Species:
- rat
- Strain:
- not specified
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- not specified
- Route of administration:
- oral: unspecified
- Vehicle:
- not specified
- Details on oral exposure:
- not specified
- Doses:
- 50 mg/kg bw
- No. of animals per sex per dose:
- not specified
- Control animals:
- not specified
- Details on study design:
- not specified
- Statistics:
- not specified
- Preliminary study:
- not specified
- Sex:
- not specified
- Dose descriptor:
- LD50
- Effect level:
- 50 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: 50% mortality was observed
- Mortality:
- 50% mortality was observed at 50 mg/kgbw
- Clinical signs:
- other: not specified
- Gross pathology:
- not specified
- Other findings:
- not specified
- Interpretation of results:
- Category 3 based on GHS criteria
- Conclusions:
- The acute oral LD50 value was considered to be 50 mg/kg bw, when rats were treated with the test chemical via oral route.
- Executive summary:
Acute oral toxicity study of the test chemical was conducted in rats at the dose concentration of 50 mg/kg bw. 50% mortality was observed. Hence, LD50 value was considered to be 50 mg/kg bw, whenrats were treated with the test chemical via oral route.
Reference
Endpoint conclusion
- Endpoint conclusion:
- adverse effect observed
- Dose descriptor:
- LD50
- Value:
- 50 mg/kg bw
- Quality of whole database:
- Klimisch rating 2
Additional information
Acute toxicity: Oral
Various studies have been reviewed to determine the acute oral toxicity in living organisms. Mostly the studies have been performed on rodents i.e rats, mice. The results are mentioned below:
Acute oral toxicity study of the test chemical was conducted in rats at the dose concentration of 50 mg/kg bw. 50% mortality was observed. Hence, LD50 value was considered to be 50 mg/kg bw, when rats were treated with the test chemical via oral route.
This is supported by another acute oral toxicity study conducted in rats for the test chemical. The test chemical at the dose concentration of 55 mg/kg bw was orally dosed to rats and observed for signs of toxicity. 50% mortality was observed. Hence, LD50 value was considered to be 55 mg/kg bw, when rats were treated with the test chemical via oral route.
These results are lent support by an acute oral toxicity study conducted in male Sprague-Dawley rats to determine the most non-toxic dose of the test chemical. The rats were orally exposed to concentration of 75 (44-127) mg/kg bw of the test chemical. Single oral dose toxicity was usually determined by the method of Smyth et al. (1962) in which the LD50 and its 95% confidence limits are estimated by the moving average technique (Thompson, 1947; Weil, 1952). 50% mortality was observed. Hence,LD50 value was considered to be 75 mg/kg bw, with 95% confidence limits of 44-127 mg/kg bw, when male Sprague-Dawley rats were treated with the test chemical via oral route.
These results are further supported by an acute oral toxicity study conducted on 80 female Sherman strain rats to determine the toxicity of the test chemical. A total of 80 rats were used for the study and the animals were individually caged during the study and fed Purina Laboratory Chow. None of them was fasted prior to dosage. The rats were individually caged in free-standing cages of stainless steel wire mesh measuring 15 inches long, 9 inches wide, and 9 inches high. The test chemical were usually formulated so that the different dosage levels of poison could be given by stomach tube by giving the formulation at a constant rate of 0.005 ml/g of body weight. The test chemical was given as a suspension in 95% ethyl alcohol orally by means of a stomach tube. The survivors were held for daily observation until they appeared to have recovered completely or for a minimum of 14 days. The poisoned rats were observed at least once each hour during the first day after dosage, and twice a day thereafter, for symptoms of poisoning and time of death. The LD50 values were determined by the method of Litchfield and Wilcoson (1949). The minimum survival time of the dosed rats was 1 day and maximum survival time was 4 days. The lowest dose to kill a female rat was 70 mg/kg. The acute oral LD50 value was considered to be 83 mg/kg bw, with 95% confidence limit of 75-91 mg/kg bw, when female Sherman strain rats were treated with the test chemical via oral gavage route.
To substantiate the claims of the above results another acute oral toxicity study was conducted in rats to determine the most non-toxic dose of the test chemical. The test chemical at the dose concentration of 90 mg/kg bw was orally dosed to rats and observed for signs of toxicity. 50% mortality was observed. Hence, LD50 value was considered to be 90 mg/kg bw, when rats were treated with the test chemical via oral route.
The results from the preceding studies are also supported by an acute oral toxicity study conducted in mice to determine the toxicity of the test chemical. The test chemical at the dose concentration of 16 (12-21) mg/kg bw was orally dosed to mice and observed for signs of mortality. 50% mortality was observed. Hence, LD50 value was considered to be16 mg/kg bw, with 95% confidence limit of 12-21 mg/kg bw, when mice were treated with the test chemical via oral route.
All of the above results are ably supported by an acute oral toxicity study performed on mice to observe the toxicity of the test chemical. 8.55 mg/kg of the test chemical was orally dosed to mice and observed for signs of mortality and clinical signs.50% mortality was observed. Clinical signs such as dyspnea, behavioral changes, muscle contraction or spasticity, tremor were observed in treated mice. Hence, LD50 value was considered to be 8.55 mg/kg bw, when mice were treated with the test chemical via oral route.
Based on the available results and applying the weight of evidence approach, the test chemical can be considered to be toxic when exposed to living organisms via oral route of exposure. The acute oral LD50 value for the test chemical lies between 50 -200 mg/kg. Comparing the above annotations with the criteria of CLP Regulation, the test chemical can be classified under the category "Category 3 for oral route”.
Justification for classification or non-classification
Based on the available results and applying the weight of evidence approach, the test chemical can be considered to be toxic when exposed to living organisms via oral route of exposure. The acute oral LD50 value for the test chemical lies between 50 -200 mg/kg. Comparing the above annotations with the criteria of CLP Regulation, the test chemical can be classified under the category "Category 3" for oral route.
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