Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 226-009-1 | CAS number: 5216-25-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- March 1980
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 980
- Report date:
- 1980
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes
- Test type:
- standard acute method
- Limit test:
- no
Test material
- Reference substance name:
- α,α,α,4-tetrachlorotoluene
- EC Number:
- 226-009-1
- EC Name:
- α,α,α,4-tetrachlorotoluene
- Cas Number:
- 5216-25-1
- Molecular formula:
- C7H4Cl4
- IUPAC Name:
- 1-chloro-4-(trichloromethyl)benzene
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: Sprague Dawley, albino, CD strain
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River UK, Kent
- Age at study initiation:6-7 weeks old on receipt
- Weight at study initiation: 176-196 g on receipt
- Housing: the rats were caged 5 males or 5 females to a cage.
- Diet: free access to a measured amount of food (Spratrt's Laboratory Diet 1)
- Water : free access to a measured amount of tap water
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 and 22 °C in the two holding rooms.
- Humidity (%): 33%, (standard deviation 5.4).
IN-LIFE DATES: From: March 3, 1980 To: March 11, 1980
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- whole body
- Vehicle:
- clean air
- Details on inhalation exposure:
- CHAMBER DESCRIPTION
The used chambers were of square section and fitted with pyramidal tops. The chambers were made of perspex and had an internal volume of approx. 0.13 m3. Each chamber was divided by wire mesh partitions to provide 10 separate animal compartments. the test atmosphere entered through a port at the base centre of the chamber and passed out through small holes in the lower edge of the square section. Each chamber was positioned inside a large glass walled cabinet equipped with an extract fan exhausting to atmosphere through a collection filter.
INHALATION EXPOSURE
Three groups of ras were exposed continously for 4 hours to test atmospheres containing the test article. A fourth group acting as a control received clean air only for 4 hours.
A supply of clean dry air was connected to the aerosol generator and the supply pressure was adjusted to give a flow rate of 30 litres per minute measured at the generator outlet tube. A 10 or 100 ml glass syringe, filled with 4-chlorobenzotrichloride was fitted to the syringe pump and connected to the generator via a three-way PTFE tap and a lenght of PTFE tubing. A flow rate of between 0.13 and 0.90 ml per minute was selected for the exposure. the rats (5 males and 5 females) were placed into separate compartments of the expsoure chamber. the syringe pump was switched on and the exposure timed from the appearence of an aerosol from the generator outlet. the syringe was refilled as required during the exposure through the three-way tap.
After 4 hours the suppluy of test article was discontinued and the exposure chamber was allowed to clear for 60 minutes before the rats were removed for examination. The exposure procedure was repeated for each test group at a difefrent setting of the syringe pump or with a different syringe to obtain other flow rates of the test substance,.
Following exposure, the rats were kept in a ventilated cabinet for 48 hours and then returned to the holding room for the remainder of the observation period.
CHAMBER ATMOSPHERE
- Particle size distribution:
Stage 1: particles freater than 5.5 µm mean aerodynamic diameter (A.d.)
Stage 2: particles between 5.5 and 2.0 µm mean aerodynamic diameter
Stage 3: particles less than 2.0 µm mean aerodynamic diameter
The temperature in the exposure chamber was measured with a mercury bulb thermometer and recorded at 30 minute intervals during the exposure. - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- samples drawn through a gas absorption trap containing 15 ml of acetonitrile at a rate of 2 litres per minute.
- Duration of exposure:
- 4 h
- Concentrations:
- 0.99, 1.31, 2.32 g/m3 of chamber air.
- No. of animals per sex per dose:
- 3 test groups, each of 5 male and 5 female rats.
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
Results and discussion
Effect levelsopen allclose all
- Sex:
- male
- Dose descriptor:
- LC50
- Effect level:
- 1.41 other: g/m3
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: Standard error 0.18 g/m3
- Sex:
- female
- Dose descriptor:
- LC50
- Effect level:
- 1.59 other: g/m3
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: Standard Error 0.25 g/m3
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- 1.48 other: g/m3
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Remarks on result:
- other: Standard Error 0.13 g/m3
- Mortality:
- Control Group : 0/5
Group 1 (mean concentration 1.31 g/m3) : 1/5 males and 3/5 females
Group 2 (mean concentration 2.32 g/m3): 5/5 males and 4/5 females
Group 3 (mean concentration 0.99 g/m3): 1/5 males and 0/5 females - Clinical signs:
- other: DURING EXPOSURE Normal appearance and behaviour for control group. Abnormal posture, lacrimation, salivation and peripheral vasodilation observed for groups exposed to 4-chlorobenzotrichloride. DURING OBSERVATION PERIOD Normal appearance and behaviour for
- Body weight:
- A loss of bodyweight over 3-4 days by all groups exposed to the test substance. Subsequent rate of bodyweight gain was irregular with a definite decrease in the rate of bodyweight gain towards the end of the observation period.
- Gross pathology:
- At the end of the 14 day observation period, the rats were anaesthetised by intraperitoneal injection of pentobarbitone sodium and killed by exsanguination. Findings for rats that died following exposure to the test substance were areas of hepatisation and bright red appearance of the lung and distended gas filled stomachs.
Findings for surviving rats were areas of hepatisation and numerous dark sub-pleural foci in the lung. - Other findings:
- Food and water consumption by each cage of rats was daily measured from the day following arrival.
Applicant's summary and conclusion
- Interpretation of results:
- other: Category 4 based on CLP criteria
- Conclusions:
- LC50 (4 hour) = 1.4 g/m3 of air
- Executive summary:
The acute inhalation toxicity of 4 -chlorobenzotrichloride was assessed by exposing 3 groups of rats for a period of 4 hours, to atmospheres containing a respirable aerosol of the test substance (whole body method). The study was performed with albino rats CD strain (SpragueDawley), females and males. One control group exposed to clean air was used too.
At the atmosphere concentartions of 0.99, 1.31 and 2.32 g/m3 of chamber air, the calculated LC50 resulted to be established at 1.48 g/m3 of air. Acute exposure to the aerosol, at the concentrations used for this study, produced irritation to the eyes and skin of the rat and had a marked injurious effect on the lungs and upper airways.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.