Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 219-529-5 | CAS number: 2455-24-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2006-12-19 to 2007-01-25
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Basic data given: comparable to guideline/standards
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guideline
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- no
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Tetrahydrofurfuryl methacrylate
- EC Number:
- 219-529-5
- EC Name:
- Tetrahydrofurfuryl methacrylate
- Cas Number:
- 2455-24-5
- Molecular formula:
- C9H14O3
- IUPAC Name:
- (oxolan-2-yl)methyl 2-methylprop-2-enoate
Constituent 1
Method
- Target gene:
- his (Salmonella strains), trp (E. coli strain)
Species / strain
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 mix
- Test concentrations with justification for top dose:
- Experiment 1: 0, 4.88, 19.5, 78.1, 313, 1250, 5000 µg/plate
Experiment 2: 0, 156, 313, 625, 1250, 2500, 5000 µg/plate - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
Controlsopen allclose all
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- other: 2-Aminoanthracene
- Remarks:
- with metabolic activation
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2-(2-Furyl)-3-(5-nitro-2-furyl)acrylamide
- Remarks:
- without metabolic activation
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in agar (plate incorporation)
DURATION
- Exposure duration: 48 h
NUMBER OF REPLICATIONS: 2
DETERMINATION OF CYTOTOXICITY
- Method: reduced bacterial growth - Evaluation criteria:
- Although not given in the study report, according to OECD guideline 471 a test item is considered mutagenic if:
- a clear and dose-related increase in revertant number occurs
- a biologically relevant positive response for at least one dose group occurs: in TA 100 and E. coli uvrA number of revertants at least twice as high as in solvent control; TA 98, TA 1535, TA 1537 at least three times higher number of revertants as in solvent control
Results and discussion
Test results
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- ADDITIONAL INFORMATION ON CYTOTOXICITY:
cytotoxic effects were observed in strains TA98, TA100, TA1535 and TA1537 at 5000 µg/plate without metabolic activation and in strains TA100 and TA1535 at 5000 µg/plate with metabolic activation - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
S9 mix |
test item concentration [µg/plate] |
mean number revertants/plate |
||||
TA100 |
TA1535 |
E. coli WP2uvrA- |
TA98 |
TA1537 |
||
without |
0 |
117 |
12 |
28 |
10 |
5 |
4.88 |
92 |
8 |
23 |
17 |
9 |
|
19.5 |
99 |
10 |
23 |
13 |
7 |
|
78.1 |
107 |
11 |
26 |
15 |
7 |
|
313 |
91 |
12 |
23 |
16 |
4 |
|
1250 |
109 |
10 |
20 |
17 |
5 |
|
5000 |
80* |
8* |
28 |
11* |
5* |
|
positive control |
722 |
404 |
257 |
414 |
138 |
|
with |
0 |
112 |
9 |
32 |
26 |
10 |
4.88 |
105 |
12 |
33 |
23 |
15 |
|
19.5 |
92 |
7 |
33 |
29 |
12 |
|
78.1 |
111 |
9 |
30 |
26 |
7 |
|
313 |
110 |
10 |
38 |
30 |
8 |
|
1250 |
124 |
7 |
34 |
24 |
10 |
|
5000 |
87* |
11* |
30 |
22 |
9 |
|
positive control |
1341 |
256 |
1017 |
421 |
124 |
S9 mix |
test item concentration [µg/plate] |
mean number revertants/plate |
||||
TA100 |
TA1535 |
E. coli WP2uvrA- |
TA98 |
TA1537 |
||
without |
0 |
106 |
13 |
16 |
14 |
7 |
156 |
113 |
14 |
24 |
12 |
4 |
|
313 |
108 |
11 |
24 |
18 |
5 |
|
625 |
88 |
15 |
21 |
18 |
10 |
|
1250 |
110 |
13 |
20 |
12 |
7 |
|
2500 |
114 |
20 |
18 |
16 |
7 |
|
5000 |
65* |
4* |
18 |
10* |
9* |
|
positive control |
679 |
454 |
188 |
520 |
195 |
|
with |
0 |
112 |
10 |
26 |
26 |
12 |
156 |
109 |
5 |
28 |
21 |
11 |
|
313 |
99 |
8 |
28 |
29 |
10 |
|
625 |
104 |
7 |
30 |
26 |
11 |
|
1250 |
112 |
11 |
36 |
22 |
10 |
|
2500 |
110 |
12 |
33 |
21 |
12 |
|
5000 |
99* |
10* |
37 |
25 |
12 |
|
positive control |
1311 |
276 |
981 |
426 |
132 |
* cytotoxic, reduced bacterial growth
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
THFMA was not mutagenic in the bacterial reverse gene mutation assay when tested up to cytotoxic or limit concentrations (5000 µg/plate). - Executive summary:
In a reverse gene mutation assay in bacteria similar to OECD guideline 471, S. typhimurium TA 1535, TA 1537, TA 98, TA 100 and E. coli WP2 strains were exposed to THFMA in DMSO at concentrations of 0, 4.88, 19.5, 78.1, 313, 1250, 5000 µg/plate in the first experiment and 0, 156, 313, 625, 1250, 2500, 5000 µg/plate in the second experiment in the presence and absence of mammalian metabolic activation (S9 mix). THFMA was tested up to limit concentrations (5000 µg/plate).
Cytotoxic effects (reduced bacterial growth) were observed in strains TA98, TA100, TA1535 and TA1537 at 5000 µg/plate without metabolic activation and in strains TA100 and TA1535 at 5000 µg/plate with metabolic activation.
There was no evidence of induced mutant colonies over background.
THFMA was not mutagenic in this bacterial reverse gene mutation assay when tested up to cytotoxic or limit concentrations (5000 µg/plate).
NOTE: Any of data in this dataset are disseminated by the European Union on a right-to-know basis and this is not a publication in the same sense as a book or an article in a journal. The right of ownership in any part of this information is reserved by the data owner(s). The use of this information for any other, e.g. commercial purpose is strictly reserved to the data owners and those persons or legal entities having paid the respective access fee for the intended purpose.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.