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EC number: 201-029-3 | CAS number: 77-47-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Description of key information
The capacity of the registered substance to induce gene mutation in bacteria and mammalian cells in vitro was investigated using methods similar to the OECD Testing Guidelines 471 and 476 respectively. The registered substance did not induce gene mutation in bacteria and mammalian cells under the conditions of these tests.
In regard to the capacity of the registered substance to induce structural chromosomal aberration in mammalian cells in vitro, it was not relevant to perform a test as in vivo studies investigating this endpoint were available.
Link to relevant study records
- Endpoint:
- in vitro gene mutation study in bacteria
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1983
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- only tested on 4 out of 5 recommended bacteria strains
- GLP compliance:
- no
- Type of assay:
- bacterial reverse mutation assay
- Target gene:
- his
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Details on mammalian cell type (if applicable):
- CELLS USED
- Source of cells: Salmonella typhimurium strains obtained from Dr. Bruce Ames, University of California, Berkeley. - Metabolic activation:
- with and without
- Metabolic activation system:
- Liver S-9 mix from Aroclor 1254-induced male Sprague-Dawley rats and male Syrian hamsters
- Test concentrations with justification for top dose:
- 0.00, 0.03, 0.10, 0.30, 1.00, 3.30, 10.00, 33.30, 100.00 ug/plate
The highest test dose was 10 mg/plate if no toxicity was apparent in the preliminary toxicity determination, or the upper limit of solubility was used. If toxicity was observed, the doses were chosen so that the high dose exhibited some degree of toxicity. - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: distilled water; dimethyl sulfoxide (DMSO); ethanol; acetone
- Justification for choice of solvent/vehicle: The first choice of solvent was distilled water. Dimethyl sulfoxide (DMSO) was used if the chemical was insoluble or not sufficiently soluble in water, and ethanol (95%) or acetone was used if the chemical was not soluble or stable in DMSO. - Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- sodium azide
- other: 2-aminoanthracene (Tested on all strains in presence of S-9); 4-nitro-o-phenylenediamine (Tes ted on TA98 without S-9)
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation
- Cell density at seeding (if applicable): 0.05ml cells added to each culture tube
DURATION
- Preincubation period: The test mixture was vortexed and allowed to incubate for 20 min at 37 °C.
- Expression time (cells in growth medium): The plates were inverted and incubated at 37°C for 48h.
NUMBER OF REPLICATIONS: At least 5 doses of each test chemical were tested on each strain in the presence of S-9 mix or buffer. Three plates were used and the experiment was repeated no less than 1 week after completion of the initial test. - Rationale for test conditions:
- The preincubation procedure was selected because of evidences that it was no less sensitive than the plate test while being more effective for various chemicals.
- Evaluation criteria:
- A 'positive' response was indicated by a reproducible, dose-related increase in histidine-independent (revertant) colonies in any one strain/activation combination. A negative response is obtained when no increase in revertant colonies is observed. An 'equivocal' or 'questionable' response was applied to low-level responses that were not reproducible or dose-related, or to results that showed a definite trend but with which the investigator did not feel comfortable making a positive or negative decision.
- Statistics:
- The data were evaluated using an analysis based on the models presented by Margolin et al (1981).
- Species / strain:
- S. typhimurium TA 98
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1535
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1537
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Positive controls validity:
- valid
- Conclusions:
- Hexachlorocyclopentadiene was not considered to be mutagenic mutagenic to Salmonella typhinurium strains TA98, TA100, TA1535 or TA 1537 in this bacterial reverse mutation assay, both with and without metabolic activation.
- Executive summary:
The in vitro genotoxicity in bacteria of the test substance was determined using a preincubation procedure, which was a modification of the Salmonella/mammalian microsome test of Ames et al [1975] and similar to the OECD Testing Guideline 471 (non GLP) with deviations.
At least five doses of test chemical, in addition to the concurrent solvent and positive controls, were tested on each bacteria strain in the presence of S-9 mix or buffer. A positive response was indicated by a reproducible, dose-related increase. Controls were valid.
Hexachlorocyclopentadiene was considered to be non-mutagenic under the conditions of the test, for both methods, with and without metabolic activation.
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Study period:
- 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 476 (In Vitro Mammalian Cell Gene Mutation Test)
- Deviations:
- yes
- Remarks:
- Low doses were used due to the substance being cytotoxic
- GLP compliance:
- no
- Type of assay:
- other: Mouse Lymphoma Forward Mutation Assay
- Target gene:
- thymidine kinase (tk)
- Species / strain / cell type:
- mouse lymphoma L5178Y cells
- Details on mammalian cell type (if applicable):
- CELLS USED
- Source of cells: Fischer mouse
MEDIA USED
- Type and identity of media including CO2 concentration if applicable: Fischer's medium for leukemic cells of mice with 10% horse serum and sodium pyruvate. - Additional strain / cell type characteristics:
- other: heterozygous for a specific autosomal mutation at the TK locus and sensitive to BUdR
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 liver
- Test concentrations with justification for top dose:
- Ranging from 2.5 pl/ml to 1.25 μl/ml. A level that showed at least 50% reduction in growth potential was used as the highest dose, with at least 3 lower doses including levels that were below the toxic range.
- Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Untreated negative controls:
- yes
- Remarks:
- growth medium without the addition of solvent
- Negative solvent / vehicle controls:
- yes
- Remarks:
- DMSO (1%)
- Positive controls:
- yes
- Positive control substance:
- N-dimethylnitrosamine
- ethylmethanesulphonate
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: 4 hours
- Expression time (cells in growth medium): 3 days
- Selection time (if incubation with a selection agent): 10 days
SELECTION AGENT (mutation assays): Bromodeoxyuridine (BUdR)
OTHER: Cloning medium consisted of Fischer's medium with 20% horse serum, sodium pyruvate, and 0.37% agar. Selection medium was made by the addition of 7.5 mg BUdR to 100 ml cloning medium. - Rationale for test conditions:
- Low doses were used due to the substance being cytotoxic.
- Evaluation criteria:
- A compound is considered mutagenic if:
- A dose-response relationship is observed over 3 of the 4 dose levels
- The minimum increase at the high level of the dose-response curve is at least 2.5 times greater than the solvent and/or negative control values
- The solvent and negative control data are within the normal range of the spontaneous background for the TK locus - Species / strain:
- mouse lymphoma L5178Y cells
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- other: cytoxicity was evaluated in order to select non cytotoxic concentrations for the assay
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not examined
- Positive controls validity:
- valid
- Conclusions:
- Hexachlorocyclopentadiene did not induce forward mutation in L5178Y cells. No dose related trends in either the absolute number of mutants or the mutant frequencies were observed and at no level did these parameters increase to 2.5 times the spontaneous level. Therefore, the compound was considered to be non-mutagenic under the conditions of this assay.
- Executive summary:
The capacity of Hexachlorocyclopentadiene to induce gene mutation in mammalian cells was determined according to the method similar to the OECD Testing Guideline 476 (non GLP) with deviations.
The test substance was evaluated in L5178Y cells with and without metabolic activation over the concentration range of 2.5 pl/ml to 1.25 µl/ml. These low concentrations were selected due to the cytotoxicity occurring at higher concentrations.
DMSO was used a the vehicle and negative control. Ethylmethanesulfonate and N-Nitrosodimethylamine were used as positive controls. Results were within the expected range and therefore considered as valid.
Hexachlorocyclopentadiene did not induce forward mutation in L5178Y cells. No dose related trends in either the absolute number of mutants or the mutant frequencies were observed and at no level did these parameters increase to 2.5 times the spontaneous level. Therefore, the compound was considered to be non-mutagenic under the conditions of this assay.
Referenceopen allclose all
See attached background material for full results table.
See attached background material for full results table.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Description of key information
The capacity of the registered substance to induce structural chromosomal aberration in vivo was investigated using methods similar to the OECD Testing Guidelines 474 and 478. The registered substance did not induce structural chromosomal aberration under the tests conditions.
Link to relevant study records
- Endpoint:
- in vivo mammalian germ cell study: cytogenicity / chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 478 (Genetic Toxicology: Rodent Dominant Lethal Test)
- Deviations:
- yes
- Remarks:
- Information on bodyweight and toxicity signs not recorded
- GLP compliance:
- no
- Type of assay:
- rodent dominant lethal assay
- Species:
- mouse
- Strain:
- CD-1
- Details on species / strain selection:
- Random bred male and female mice
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River Breeding Laboratories, Inc., Protage, Michigan
- Age at study initiation: at least 8 weeks
- Assigned to test groups randomly: yes
- Housing: Males housed individually and females housed in pairs (except when mating) in shoe box cages on AB-SORB-DRI bedding.
- Diet: Purina Lab Chow ad libitum
- Water: ad libitum
- Acclimation period: 2 weeks
ENVIRONMENTAL CONDITIONS
- Photoperiod: 12 hour light/dark cycle
- Route of administration:
- oral: gavage
- Vehicle:
- - Vehicle(s)/solvent(s) used: DMSO
- Duration of treatment / exposure:
- Male mice exposed to several dose levels of test compound over 5 days
- Frequency of treatment:
- Daily
- Post exposure period:
- Rested for 2 days following treatment, then mated weekly for 7 weeks.
- Dose / conc.:
- 1 mg/kg bw/day (nominal)
- Dose / conc.:
- 0.3 mg/kg bw/day (nominal)
- Dose / conc.:
- 0.1 mg/kg bw/day (nominal)
- No. of animals per sex per dose:
- 10 random bred mice assigned to 1 of 5 groups. Three groups received different dose levels of hexachlorocyclopentadiene; a fourth group received only the solvent; a fifth group received a known mutagen as the positive control. Following treatment, each male was mated with 2 virgin females which were replaced weekly for 7 weeks.
- Control animals:
- yes, concurrent vehicle
- yes, sham-exposed
- yes, historical
- Positive control(s):
- Triethylenemelamine (TEM)
- Justification for choice of positive control(s):
- Route of administration: single intraperitoneal injection - Details of tissue and slide preparation:
- CRITERIA FOR DOSE SELECTION: Range finding studies were conducted to calculate dose information. LD50, LD5 and LD1 concentrations were computer generated based on the preliminary study. The high dose level was selected from these data; 1/3rd and 1/10th of the high dose were used as the intermediate and low dose levels respectively.
TREATMENT AND SAMPLING TIMES: Male mice were exposed to the test compound for 5 days then mated over the entire period of spermatogenesis to unexposed virgin females. At mid-pregnancy the females were killed and scored for number of living and dead implants, as well as fertility level.
METHOD OF ANALYSIS: The number of dead and living fetuses, resorption sites, and total implantation sites were recorded. Data were analysed for statistical significance. Results were compared to data from control animals and used to determine the degree of induced dominant lethality. - Evaluation criteria:
- Dominant lethality is determined from:
a) a mutation index derived from dead to total implants; or
b) the number of dead implants per pregnant female - Statistics:
- See attached background material.
- Key result
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- yes
- Remarks:
- A preliminary test was performed in order to determine the toxicity of the substance and adapt the final doses selected
- Vehicle controls validity:
- valid
- Negative controls validity:
- valid
- Positive controls validity:
- valid
- Additional information on results:
- RESULTS OF RANGE-FINDING STUDY
- Dose range: 50 mg/kg to 5000 mg/kg (first trial). 7.6 mg/kg to 76 mg/kg (second trial).
- Clinical signs of toxicity in test animals: Mortality occurred at all the doses investigated. All animals died at 76 mg/kg and above. - Conclusions:
- Hexachlorocyclopentadiene was not active in this Mouse Dominant Lethal Assay.
- Executive summary:
A mouse dominant lethal assay was performed to determine the potential of the assay to induce structural and numerical chromosome aberrations according to a method similar to the OECD Tetsing Guideline 478 (non-GLP) with deviations.
Hexachlorocyclopentadiene was tested for toxicity over a range of 50 mg/kg to 5,000 mg/kg. Based on this toxicity and acute LD50 level of 76 mg/kg was calculated. The high dose was selected as the LD50 and concentrations of 25.3 mg/kg and 7.6 mg/kg were employed as the two lower doses levels. These concentrations proved to be too toxic for a five-day exposure since all ten animals died before five days. A second LD50 was calculated and identified 1 mg/kg as the high dose and 1/3 and 1/10 of the LD50 levels for the study.
The test material was examined for its ability to induce dominant lethality in mice treated with 0.1, 0.3 and 1.0 mg/kg bw/d for five days.
There was no evidence for significant dominant lethal activity by Hexachlorocyclopentadiene in mice. All data were within the concurrent and historical control levels. The positive control compound produced the expected dominant lethal effects in the first three mating weeks. Hexachlorocyclopentadiene was not active in the Mouse Dominant Lethal Assay conducted as part of this evaluation.
- Endpoint:
- in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 474 (Mammalian Erythrocyte Micronucleus Test)
- Deviations:
- yes
- Remarks:
- No data on animal toxicity.
- GLP compliance:
- no
- Type of assay:
- other: mammalian erythrocyte micronucleus test
- Species:
- mouse
- Strain:
- B6C3F1
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Frederick Cancer Research Facility
- Females (if applicable) nulliparous and non-pregnant: yes
- Age at study initiation: 6 weeks
- Weight at study initiation: Females: 101 to 108g / Males: 118 to 127g
- Housing: individually in stainless steel cages (Hazleton Systems)
- Diet (e.g. ad libitum): NIH-07 pelleted rodent diet ad libitum
- Water (e.g. ad libitum): Tap water ad libitum
- Acclimation period: 2 weeks
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20°C-21°C
- Humidity (%): 35%-65%
- Air changes (per hr): 20 changes/h
- Photoperiod (hrs dark / hrs light): 12h/12h - Route of administration:
- inhalation: vapour
- Vehicle:
- - Vehicle(s)/solvent(s) used: air
- Details on exposure:
- GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: Stainless steel whole-body inhalation chamber (Hazleton Systems)
- Source and rate of air: fresh air
- System of generating particulates/aerosols: Vaporizer. Gardner Type CN condensation nuclei detector used to ensure the generation of vapour and not of aerosol.
TEST ATMOSPHERE
- Brief description of analytical method used: On-line gas chromatograph with electron capture detector.
- Samples taken from breathing zone: yes - Duration of treatment / exposure:
- 13 weeks
- Frequency of treatment:
- 6 hours per day, 5 days per week
- Dose / conc.:
- 0 ppm (nominal)
- Dose / conc.:
- 0.01 ppm (nominal)
- Remarks:
- Equivalent to 0.08 mg/m3.
- Dose / conc.:
- 0.05 ppm (nominal)
- Remarks:
- Equivalent to 0.39 mg/m3.
- Dose / conc.:
- 0.2 mg/kg bw/day (nominal)
- Remarks:
- Equivalent to 1.56 mg/m3.
- No. of animals per sex per dose:
- 10
- Control animals:
- yes, concurrent vehicle
- Positive control(s):
- no data
- Tissues and cell types examined:
- Micronuclei in polychromatic erythrocytes (PCEs) and normochromatic erythrocytes (NCEs).
- Details of tissue and slide preparation:
- DETAILS OF SLIDE PREPARATION: Peripheral blood samples were obtained from mice after 13-week inhalation toxicity study. Smears were immediately prepared and fixed in absolute methanol, and later stained with a chromatin-specific fluorescent dye mixture of Hoechst 33258/pyronin Y.
METHOD OF ANALYSIS: Slides scanned to determine the frequency of micronuclei in 2,000 polychromatic erythrocytes (PCEs) and 10,000 normochromatic erythrocytes (NCEs). Percentage of PCEs amoung the total erythrocyte population was also determined. - Evaluation criteria:
- The criteria of Schmid (1976) were used to define micronuclei. It was also required that the micronuclei exhibit the characteristic fluorescent emissions of DNA; the minimum size limit was approximately one-twentieth the diameter of the NCE cell.
- Statistics:
- Before statistical analysis, log transformation of the NCE data, testing for normality by Shapiro-Wilk test, and heterogeneity of variance by Cochran's test were performed. Frequency of micronucleated cells amoung NCEs was analysed by analysis of variance using the SAS GLM procedure. The Student's t-test was used to compare the NCE data for each dose group with the concurrent solvent control. The Cochran-Armitage trend test was used to analyse the frequency of micronucleated cells amoug PCEs, and individual dose groups were compared to the concurrent solvent control by Kastenbaum-Bowman's bionomial test. The percentage of PCEs among total erythrocytes was analysed by an analysis of variance on ranks (classed by sex), and individual dose groups were compared with the concurrent solvent control using a t-test on ranks.
- Sex:
- male/female
- Genotoxicity:
- negative
- Toxicity:
- not examined
- Remarks:
- Doses were selected based on previous toxicological testing
- Vehicle controls validity:
- valid
- Positive controls validity:
- not examined
- Additional information on results:
- RESULTS OF DEFINITIVE STUDY
- Induction of micronuclei (for Micronucleus assay): No increase in the frequency of micronucleated erythrocytes was observed in peripheral blood samples obtained from male and female B6C3Fl mice exposed to hexachlorocyclopentadiene by inhalation for 13 weeks
- Ratio of PCE/NCE (for Micronucleus assay): See attached background information.
- Statistical evaluation: Exposed groups do not differ from the control (by Student's t-test, Kastenbaum-Bowman's binomial test, or t-test on ranks). - Conclusions:
- No increase in the frequency of micronucleated erythrocytes was observed in peripheral blood samples obtained from male and female B6C3F1 mice exposed to hexachlorocyclopentadiene by inhalation for 13 weeks. It was concluded that the substance did not induce structural chromosomal aberrations under the conditions of the study.
- Executive summary:
The potential of Hexachlorocyclopentadiene to induce structural chromosomal aberration in mice was determined by the US NTP, using a Mouse Peripheral Blood Micronucleus Test protocol. The study is not GLP compliant however the procedure is comparable to the OECD Testing Guideline 474. Male and female B6C3F1 mice were exposed to hexachlorocyclopentadiene by inhalation for 13 weeks, at doses of 0.00, 0.01, 0.05, and 0.20 ppm. After treatment peripheral blood samples were taken. Smears were prepared and fixed in absolute methanol; later they were stained with a chromatin-specific fluorescent dye. Slides were scanned to determine micronuclei frequency in 2,000 polychromatic erythrocytes (PCEs) and 10,000 normochromatic erythrocytes (NCEs) in 10 animals per dose group. Percentage of PCEs among the total erythrocyte population was determined. The results were analysed using a variety of statistical tests, and there was found to be no increase in the frequency of micronucleated erythrocytes observed in the peripheral blood samples. It was concluded that the substance did not induce structural chromosomal aberrations under the conditions of the study.
Referenceopen allclose all
See attached background material for full results table.
See attached background material.
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Justification for classification or non-classification
The genotoxicity studies examined were non GLP compliant however they followed procedures similar to the OECD Guidelines for Testing of Chemicals, with acceptable restrictions. Results of these studies performed on Hexachlorocyclopentadiene were negative. Therefore the substance did not meet the criteria for classification as mutagenic according to Regulation (EC) N° 1272/2008.
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