Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 269-085-1 | CAS number: 68187-30-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study with acceptable restrictions (test substance concentration not analytically verified)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Direct addition by initial weight into the test flasks. The testsubstance was weighed in the required amounts for the test concentrations directly to the test vessels. - Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- - Laboratory culture: Activated sludge from the municipal wastewater treatment plant of Mannheim, Germany was collected on 05 Jun 2012 from the aeration tank of the plant.
- Preparation of inoculum for exposure: The activated sludge suspension was sieved with a fine woven mesh (mesh size about 1 mm). The suspension was pre-aerated over night at room temperature. At the next day the sludge suspension was washed once with drinking water. The suspension was adjusted to 3 g/L dry matter. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 20.8 - 21.3 °C
- pH:
- 7.1 - 7.4 (at the start)
7.7 - 8.0 (after incubation time) - Dissolved oxygen:
- > 2 mg/L (during aeration)
> 7 mg/L (immediately before measurement) - Nominal and measured concentrations:
- Nominal concentration: 118, 236, 472, 943 and 1887 mg/L (based on test substance)
Nominal concentration: 62.5, 125, 250, 500 and 1000 mg/L (based on active matter) - Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Material: glass: Size: 1 L; Fill volume: 500 mL
- No. of vessels per concentration: 2
- No. of vessels per control: 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: deionized water
- Culture medium different from test medium: 16 mL/test vessel of 100-fold concentrated OECD medium
- Intervals of water quality measurement: The pH was measured at the beginning and at the end of the test. Oxygen concentration was measured during aeration and immediately before measurement.
OTHER TEST CONDITIONS
- Adjustment of pH: yes
EFFECT PARAMETERS MEASURED: Oxygen consumption was measured for a period of 8 - 10 minutes after a incubation time of 3 h. - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- - Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: none
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 = 7 mg/L
- Other: The reference substance shows an EC50 in the range of 2 - 25 mg/L in 3 h. This indicates that the microorganisms are responding normally to toxicant stress. - Validity criteria fulfilled:
- yes
Reference
Description of key information
EC10 (3h) > 1000 mg/L (nominal, based on active matter) for activated sludge (OECD 209)
Key value for chemical safety assessment
Additional information
One study investigating the toxicity of l-Glutamic acid, N-coco acyl derivs., disodium salts (CAS No. 68187-30-4) to aquatic microorganisms is available. The study was performed according to GLP and OECD guideline 209 using non-adapted activated sludge as inoculum (BASF, 2012). Nominal concentrations between 118 and 1887 mg/L were tested, which correspond to test concentrations of 62.5 to 1000 mg/L based on active matter. No respiration inhibition compared to the control was observed in any treatment. Thus, a 3h-EC10 (based on active matter) is determined to be > 1000 mg/L.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.