Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 425-430-1 | CAS number: 6746-94-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12 March to 7 April 1997
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 997
- Report date:
- 1997
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Principles of method if other than guideline:
- The study design was in compliance with US EPA, EEC and OECD test guidelines for acute inhalation studies .
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- no
Test material
- Reference substance name:
- -
- EC Number:
- 425-430-1
- EC Name:
- -
- Cas Number:
- 6746-94-7
- Molecular formula:
- C5 H6
- IUPAC Name:
- ethynylcyclopropane
- Details on test material:
- - Name of test material (as cited in study report): EthynyI cyclopropane (SD 957)
- Physical state: Colourless liquid
- Analytical purity: 97-100 %
- Lot/batch No.: 3590-129-D4
- Expiration date of the lot/batch: January 1999
- Storage condition of test material: -20°C, under nitrogen and in the dark
- Intended use: Pharmaceutical intermediate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Sprague-Dawley
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Charles River UK Limited, Manston Road, Margate, Kent, England
- Age at study initiation: four to six weeks
- Weight at study initiation: 200 to 300 g
- Fasting period before study: none
- Housing: holding cages (size 35 cm x 53 cm x 25 cm height) were made of stainless steel sheet and wire mesh; housed by sex in groups of 5
- Diet: SDS (Special Services Diet Ltd) rat diet (RM1) ad libitum
- Water: drinking water ad libitum
- Acclimation period: for a minimum period of five days prior to the start of the study
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 ± 3
- Humidity (%): 55 ± 15
- Air changes (per hr): 12 to 15
- Photoperiod (hrs dark / hrs light): 12/12
The batch(s) of diet used for the study was analysed for certain nutrients, possible contaminants and microorganisms.
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Vehicle:
- other: unchanged (no vehicle)
- Details on inhalation exposure:
- EXPOSURE SYSTEM
Vapour Generator
The atmosphere generator was designed to produce and maintain an atmosphere containing vapour by evaporation of the test substance from the sintered glass disc with a countercurrent of air. All parts of the generator in contact with the test substance were made of glass. The test substance was delivered to the generator at a constant flow rate from a syringe driven by a syringe pump and the air supplied to the generator was dried, filtered and oil free.
Exposure chambers
The whole-body exposure chambers (ADG Developments ) used for the exposures were of square section (size 51cm x 51cm x 38cm height) and were made of acrylic polymer. The chambers were fitted with a pyramidal top section and had an enclosed volume of 120 liters. The rats were held for exposure in a stainless steel mesh exposure cage, subdivided to provide individual compartments for the rats. The test atmosphere entered the chamber through a port at the top centre of the chamber and was extracted at the base centre below the level of the rats. Each chamber was installed in a large fume
cupboard exhausting through an absolute filter.
PROCEDURE
A supply of clean dried air was connected to the vapour generator and the supply pressure was adjusted to give a flow rate of 25 liters per minute measured at the generator outlet tube. An in-line flow meter was used to monitor air flow throughout the exposure period. A syringe filled with the test substance was fitted to the syringe pump and connected to the generator with PTFE tubing. An initial flow rate of 0 .8 ml/min was selected for the first exposure. This flow rate was expected to give a nominal concentration of 20 mg/l in air. The flow rate of SD957 was adjusted as necessary to avoid an excessive amount of liquid on the sintered glass disc. The rats to be exposed were placed into separate compartments of the exposure chamber. The syringe pump and air supply was switched on and the exposure timed for 4 hours, following an 11 minute equilibration period. After 4 hours, the test substance was discontinued and the exposure chamber was ventilated with clean air for approximately 11 minutes to clear the test article from the chamber before the rats were removed for examination.
The procedure was repeated, for the exposure of Groups 3 and 4, using flow rates of SD957 of 0.11 and 0.018 ml/min respectively. Following exposure, the rats were returned to the holding cages and food and water supplies were restored. The test rats were kept in a ventilated cabinet overnight, separate from the control animals, and then returned to the holding room for the remainder of the observation period. The control group was treated similarly but exposed to air only for 4 hours. The control rats were returned to the holding room at the end of the exposure procedure. - Analytical verification of test atmosphere concentrations:
- yes
- Remarks:
- by gas chromatography
- Duration of exposure:
- 4 h
- Concentrations:
- 0; 0.5; 3.22; 23.3 mg/l air
- No. of animals per sex per dose:
- 5 males, 5 females
- Control animals:
- yes
- Details on study design:
- - Duration of observation period following administration: 14 days
- Other examinations performed: clinical signs, body weight, food and water consumption
Clinical signs
The rats were observed continuously for signs of reaction to the test substance during exposure and at least twice daily throughout the post-exposure period. The clinical signs were recorded at the end of the chamber equilibration period, at 0.25, 0.5 and 1.0 hours, then at hourly intervals during the exposure. During the observation period, a full clinical signs check was performed twice daily. The first check was within 1- 2 hours of the time of day of the commencement of the exposure, the second check was 6 hours later.
Bodyweight
All rats were weighed daily from the day of delivery to the Huntingdon Life Sciences until the end of the observation period.
Food and water consumption
The amount of food and water consumed by each cage of rats was measured daily from the day of arrival. The daily mean intakes of food and water for each rat were calculated from the total consumed per cage divided by the number of rats per cage giving a consumption in g/rat . Food consumption values were not adjusted for spillage.
TERMINAL STUDIES
At the end of the 14-day observation period, the rats were killed by intraperitoneal injection of phentobarbitone sodium and exsanguinated when clinically dead. All rats were subjected to a detailed macroscopic examination. The lungs were removed, with the trachea (cut below the larynx) attached, dissected clear of surrounding tissue and the trachea, bronchi and both lungs weighed in order to calculate the lung weight to bodyweight ratio. Following weighing, the lungs were infused with, and preserved in, buffered 10% formalin and ligated prior to preservation together with samples of the liver and kidneys for possible future microscopic examination.
Results and discussion
Effect levelsopen allclose all
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- > 22.3 mg/L air (analytical)
- Exp. duration:
- 4 h
- Sex:
- male/female
- Dose descriptor:
- other: NOEC
- Effect level:
- > 0.5 - < 3.22 mg/L air (analytical)
- Exp. duration:
- 4 h
- Mortality:
- There were no deaths following exposure to SD957.
- Clinical signs:
- other: During the exposure - During the exposure, signs seen in rats exposed to SD957 at 22.3 mg/l included unsteady movement, occasional involuntary muscular contractions, lack of response to stimuli, piloerection, partial closing of the eyes and shallow respir
- Body weight:
- There were no test article related effects on mean bodyweight gain following exposure to SD957.
- Gross pathology:
- Lung weight to bodyweight ratio
The lung weight to bodyweight ratio for test animals exposed to SD957 were similar to those of the controls.
Macroscopic pathology
One male and one female rat exposed at 22 .3 mg/l of SD957 had minimal congestion of all lobes of the lungs at post mortem. There were no other macroscopic abnormalities noted in test or control rats. - Other findings:
- Food consumption
There was a slight reduction in mean food consumption for one day following exposure to SD957 at 22.3 mg/l. There were no meaningful differences between mean food consumption of SD957-exposed and control rats during the study period.
Water consumption
There were no meaningful differences between mean water consumption of SD957-exposed and control rats during the study period .
Any other information on results incl. tables
CONCLUSION
The LC50 (4-hour) for SD957 is in excess of 22.3 mg/l of air . The no effect concentration (NOEC) is in excess of 0.50 mg/l but less than 3.22 mg/l of air.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.