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EC number: 608-477-2 | CAS number: 3041-16-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2017-12-18 to 2018-01-19
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- ISO 10707 Water quality - Evaluation in an aqueous medium of the "ultimate" aerobic biodegradability of organic compounds - Method by analysis of biochemical oxygen demand (closed bottle test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 1,4-dioxan-2-one
- EC Number:
- 608-477-2
- Cas Number:
- 3041-16-5
- Molecular formula:
- C4H6O3
- IUPAC Name:
- 1,4-dioxan-2-one
- Test material form:
- solid
- Details on test material:
- - Physical state: powder/solid
- Appearance: white powder
Constituent 1
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Analytical purity: 99.86%
- Source and lot/batch No.of test material: 397451
- Expiration date of the lot/batch: 2018-02-07 (retest date)
- Purity test date: 2017-09-05 (certificate of analysis release date)
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: In refrigerator (2-8°C)
- Solubility in water: 20 g/L
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source: Municipal sewage treatment plant receiving predominantly domestic sewage, 'Waterschap Aa en Maas', 's-Hertogenbosch, The Netherlands.
- Storage conditions: Sludge was kept under continuous aeration until further treatment
- Preparation of inoculum for exposure: The secondary effluent was filtered through a coarse filter paper and the first 200 mL were discarded. The filtrate was be kept aerated until inoculation.
- Pretreatment: no
- Concentration of sludge: 4 mL filtrate of secondary effluent per litre of final volume.
- Water filtered: Tap-water purified by reverse osmosis (Millipore Corp., Bedford, Mass., USA). - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 2 mg/L
- Based on:
- test mat.
- Initial conc.:
- 5 mg/L
- Based on:
- test mat.
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Tap-water purified by reverse osmosis (Millipore Corp., Bedford, Mass., USA).
* mineral stock solution A: 8.5 g KH2PO4, 21.75 g K2HPO4, 67.20 g Na2HPO4.12H2O, 0.5 gNH4Cl dissolved in 1 L Milli-Q water, pH 7.4 ± 0.2
* mineral stock solution B: 22.50 g MgSO4.7H2O dissolved in 1 L Milli-Q water
* mineral stock solution C: 36.4 g CaCl2.2H2O dissolved in 1 L Milli-Q water
* mineral stock solution D: 0.25 g FeCl3.6H2O dissolved in 1 L Milli-Q water
* Final test medium: 1 mL of solutions A to D per L of test medium
- Additional substrate: no
- Test temperature: 19-21°C
- pH: 7.1-7.2, At the start of the test
- pH adjusted: no
- Aeration of dilution water: The test solutions were continuously aerated and stirred during the test.
- Continuous darkness: yes
TEST SYSTEM
- Culturing apparatus: 250-300 mL BOD bottles with glass stoppers.
- Number of culture flasks/concentration: 2
* test substance and inoculum: 2 replicates
* inoculum blank: 2 replicates
* positive control: 2 replicate
* toxicity control: 2 replicate
- Method used to create aerobic conditions: The mineral medium was left at test temperature to obtain an oxygen-saturated solution at the start of the test. Care was taken to ensure the solutions were not supersaturated with oxygen.
- Measuring equipment: WTW oxygen meter supplied with a WTW CellOx 325 oxygen electrode, electrolyte type ELY/G.
SAMPLING
- Sampling frequency: In duplicate; immediately at the start of the experiment (day 0), and on day 7, 14, 21 and 28.
- Sampling method: no data
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes, with only inoculum
- Toxicity control: yes, with test item, reference substance (lowest concentration), and inoculum
- Procedure control: yes, with reference item and inoculum
Reference substance
- Reference substance:
- acetic acid, sodium salt
Results and discussion
- Test performance:
- - In the toxicity control more than 25 % degradation occurred within 14 days (51% based on ThCO2). Therefore, the test substance was assumed to be not inhibitory on microbial activity.
- Oxygen depletion in the inoculum blank was less than 1.5 mg O2/L after 28 days (0.65 mg O2/L).
- The residual concentration of oxygen in the test bottles was > 0.5 mg/L at any time.
- Differences between duplicate biodegradation values (expressed as mg O2/L) were less than 20% (7-8%).
- In the procedure control, sodium acetate was degraded by at least 60% (79%) within 14 days.
% Degradationopen allclose all
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 94
- Sampling time:
- 28 d
- Remarks on result:
- other: initial concentration of 2.0 mg/L
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 60
- Sampling time:
- 28 d
- Remarks on result:
- other: initial concentration of 5.0 mg/L
- Details on results:
- The relative biodegradation values calculated from the O2 measurements performed during the test period of 28 days revealed 94% and 60% biodegradation of 1,4-Dioxan-2-one (PDO Monomer) (based on ThOD) at the concentration of 2.0 mg/L and 5.0 mg/L, respectively. In addition, at a concentration of 2.0 mg/L biodegradation of at least 60% was reached within 14 days of biodegradation exceeding 10%. No explanation can be given for the decreasing biodegradation pattern at 5.0 mg/L as of day 21 of the test.
BOD5 / COD results
- Results with reference substance:
- The positive control item was biodegraded by at least 60% (79%) within 14 days, confirming suitability of the activated sludge.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- A 28-d ready biodegradability test (OECD 301D, modified Sturm test) using unadapted activated sludge from a predominantly domestic waste water treatment plant indicated that 1,4-dioxan-2-one was readily biodegradable under the conditions of the test (initial concentration of 2 and 5 mg/L). The test substance showed 94% and 60% biodegradation (at the concentration of 2.0 mg/L and 5.0 mg/L, respectively). The test substance did not inhibit microbial activity at the concentration used in the test. The results of the test can be considered reliable without restriction.
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