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EC number: 200-662-2 | CAS number: 67-64-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Endpoint summary
Administrative data
Key value for chemical safety assessment
Genetic toxicity in vitro
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Genetic toxicity in vivo
Endpoint conclusion
- Endpoint conclusion:
- no adverse effect observed (negative)
Additional information
Genotoxicity in vitro:
No genotoxicity activity of acetone was evident in vitro in the following three key studies:
The genotoxic potential of acetone was investigated in Salmonella typhimurium strains TA 1535, TA 1537, TA 97, TA 98 and TA 100 with and without metabolic activation with a test protocol comparable to OECD guideline 471. Test results were negative except for a questionable result with the strain TA 97 occurring only after metabolic activation with 30 % rat liver S-9 but not in the presence hamster liver S-9 or 10 % rat liver S-9. In summary, there was no evidence of a genotoxic potential of acetone in the Salmonella typhimurium reverse mutation assay with and without metabolic activation up to a concentration of 10 mg/plate (NTP, 1986; Zeiger et al., 1992). In an in vitro chromosome aberration test in CHO cells with and without metabolic activation according to a test protocol similar to OECD Guideline no increased incidence of structural aberrations was found up to concentrations of 5 mg acetone/mL (Loveday et al., 1990; NTP, 1986). The absence of a mutagenic potential of acetone was indicated in the mouse lymphoma mutagenicity assay performed only in the absence of a metabolic activation system with a test protocol similar to OECD Guideline 476 (Amacher et al., 1980).
Weight of evidence further confirms the absence of genotoxic activity from further genotoxicity assays (For this compilation the reliability of the individual studies was not checked or the reliability may be 3 or 4). There were no indications of a genotoxic potential in the following test systems:
- reverse mutation assay in S. typhimurium
- SOS chromotests in S. typhimurium and E. coli
- assays on prophage induction or DNA binding in E. coli
- recombination assay in B. subtilis
- chromosomal malsegregation, point mutations and mitotic recombination in S. cerevisiae
- forward mutation in S. pombe
- mutagenicity assay in mouse lymphoma cells and Chinese hamster lung fibroblasts
- chromosomal aberration in CHO cells and human lymphocytes
- sister chromatid exchange in CHO cells and human lymphocytes
- unscheduled DNA synthesis in human skin cells and bovine lymphocytes
- DNA strandbreaks in rat hepatocytes
Genotoxicity in vivo:
No genotoxic activity of acetone was evident in vivo based on a weight of evidence approach:
Groups of 10 B6C3F1 mice, that had continuously been exposed to 0, 0.5, 1 or 2 % acetone in drinking water for 13 weeks, were scored for induction of micronuclei in normochromatic erythrocytes in peripheral blood. Based on actual water consumption these concentrations correspond to dosages of 1,569, 3,023, and 5,481 mg/kg bw/d in male mice and dosages of 2,007, 4,156, and 5,945 mg/kg bw/d in female mice (values reported in NTP, 1991). No indication of a clastogenic activity of acetone was found (NTP, 1993).
A dose of 865 mg acetone/kg bw (2/3 of the LD50) was intraperitoneally administered to Chinese hamsters (5 males and 5 females per timepoint) and the percentage of polychromatic bone marrow erythrocytes with micronuclei was investigated at sampling timepoints of 12, 24, 48, and 72 hours. There was no increase of the rate of micronuclei at any timepoint (Basler, 1986).
Short description of key information:
Bacterial mutagenicity assay: Salmonella typhimurium strains TA
1535, TA 1537, TA 97, TA 98 and TA 100 with and without metabolic
activation (OECD Guideline 471): negative (Key study: NTP, 1986)
Mammalian cell mutagenicity assay in vitro: mouse lymphoma cells without
metabolic activation (OECD Guideline 476): negative (Key study: Amacher
et al., 1980)
Mammalian cell clastogenicity assay in vitro: chromosome aberration test
in CHO cells with and without metabolic activation (OECD Guideline 473):
negative (Key study: NTP, 1986)
In vivo micronucleus test in peripheral blood erythrocytes: mouse, 0,
0.5, 1 or 2 % acetone in drinking water for 13 weeks: negative (Weight
of evidence: NTP, 1993)
In vivo micronucleus test in bone marrow erythrocytes: Chinese hamster,
865 mg acetone/kg bw i.p.: negative (Weight of evidence: Basler, 1986)
Endpoint Conclusion: No adverse effect observed (negative)
Justification for classification or non-classification
There is no classification for genetic toxicity based on negative test results in in vitro and in vivo test systems.
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