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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation
Remarks:
in vitro
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Deviations:
no
GLP compliance:
yes (incl. certificate)

Test material

Reference
Name:
Unnamed
Type:
Constituent
Type:
Constituent

Test system

Vehicle:
water

Results and discussion

In vivo

Results
Irritation parameter:
overall irritation score
Basis:
mean
Time point:
other: 300s
Score:
7.5
Max. score:
7.9
Reversibility:
not specified

Any other information on results incl. tables

Test Group

Irritation Score

MeanIrritation Score

NegativeControl

0.00

0.00

NegativeControl

0.00

 

NegativeControl

0.00

 

PositiveControl 0.1NaOH

19.21

19.00

PositiveControl 0.1NaOH

18.98

 

PositiveControl 0.1NaOH

18.82

 

PositiveControl 1%SDS

10.53

10.07

PositiveControl 1%SDS

9.84

 

PositiveControl 1%SDS

9.85

 

BHT-2NH35,5'-Bis-1H-tetrazolediammoniumsalt

7.54

7.50

BHT-2NH3 5,5'-Bis-1H-tetrazolediammoniumsalt

7.22

 

BHT-2NH3 5,5'-Bis-1H-tetrazolediammoniumsalt

6.79

 

BHT-2NH3 5,5'-Bis-1H-tetrazole *diammoniumsalt

7.79

 

BHT-2NH3 5,5'-Bis-1H-tetrazolediammoniumsalt

8.64

 

BHT-2NH35,5'-Bis-1H-tetrazolediammoniumsalt

7.02

 

 

Applicant's summary and conclusion

Interpretation of results:
moderately irritating
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
This in vitro study was performed to assess the irritating potential of BHT-2NH3 5,5'-Bis-1H-tetrazole diammonium salt by detection of damages in blood vessels under the chorionallantoic membrane of incubated chicken eggs (nine days old). Observation time was five minutes at room temperature.
Physiological sodium Chloride Solution was used as negative control, sodium dodecyl Sul¬fate (1% Solution) and sodium hydroxide (0.1-n Solution) were used as positive controls.
The positive controls induced a severe irritation on the blood vessels. The negative control
showed no irritation, the irritation score for the positive controls lay within the demanded
ränge.
The test item was tested pure. A mean irritation score of 7.50 was calculated, correspond-
ing to a Classification as moderately irritant.
No observations were made which might cause doubts concerning the validity of the study
outcome. The test is considered valid.